##gff-version 3
##sequence-region BBa_J01059 1 1888
BBa_J01059	.	terminator	784	863	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_J01059	.	stem_loop	795	838	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_J01059	.	engineered_region	784	863	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_J01059	.	CDS	1	750	.	+	0	ID=BBa_C0051;Name=BBa_C0051
BBa_J01059	.	tag	712	744	.	+	0	ID=annotation23335;Name=LVA;Parent=BBa_C0051
BBa_J01059	.	engineered_tag	751	775	.	+	0	ID=annotation2213991;Name=Help:Barcodes;Parent=BBa_C0051
BBa_J01059	.	CDS	4	711	.	+	0	ID=annotation23334;Name=cI lambda;Parent=BBa_C0051
BBa_J01059	.	terminator	872	912	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_J01059	.	engineered_region	872	912	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_J01059	.	stop_codon	905	905	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_J01059	.	polyA_site	899	912	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_J01059	.	stem_loop	879	898	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_J01059	.	terminator	1760	1839	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_J01059	.	stem_loop	1771	1814	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_J01059	.	engineered_region	1760	1839	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_J01059	.	CDS	1032	1751	.	+	0	ID=BBa_E0040;Name=BBa_E0040
BBa_J01059	.	CDS	1032	1751	.	+	0	ID=annotation1934520;Name=GFP protein;Parent=BBa_E0040
BBa_J01059	.	promoter	921	1002	.	+	0	ID=BBa_I12007;Name=BBa_I12007
BBa_J01059	.	promoter	991	996	.	+	0	ID=annotation937705;Name=-10;Parent=BBa_I12007
BBa_J01059	.	sequence_alteration	979	985	.	+	0	ID=annotation937704;Name=mutate to obliterate OR3;Parent=BBa_I12007
BBa_J01059	.	non_covalent_binding_site	953	969	.	+	0	ID=annotation937702;Name=OR2;Parent=BBa_I12007
BBa_J01059	.	promoter	968	973	.	+	0	ID=annotation937703;Name=-35;Parent=BBa_I12007
BBa_J01059	.	non_covalent_binding_site	929	945	.	+	0	ID=annotation937701;Name=OR1;Parent=BBa_I12007
BBa_J01059	.	terminator	1848	1888	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_J01059	.	engineered_region	1848	1888	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_J01059	.	stop_codon	1881	1881	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_J01059	.	polyA_site	1875	1888	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_J01059	.	stem_loop	1855	1874	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_J01059	.	ribosome_entry_site	1011	1025	.	+	0	ID=BBa_B0030;Name=BBa_B0030
BBa_J01059	.	engineered_region	1011	1025	.	+	0	ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030
BBa_J01059	.	ribosome_entry_site	1011	1025	.	+	0	ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030
BBa_J01059	.	ribosome_entry_site	1018	1022	.	+	0	ID=annotation1702;Name=RBS;Parent=BBa_B0030
>BBa_J01059
atgagcacaaaaaagaaaccattaacacaagagcagcttgaggacgcacgtcgccttaaagcaatttatgaaaaaaaga
aaaatgaacttggcttatcccaggaatctgtcgcagacaagatggggatggggcagtcaggcgttggtgctttatttaa
tggcatcaatgcattaaatgcttataacgccgcattgcttgcaaaaattctcaaagttagcgttgaagaatttagccct
tcaatcgccagagaaatctacgagatgtatgaagcggttagtatgcagccgtcacttagaagtgagtatgagtaccctg
ttttttctcatgttcaggcagggatgttctcacctgagcttagaacctttaccaaaggtgatgcggagagatgggtaag
cacaaccaaaaaagccagtgattctgcattctggcttgaggttgaaggtaattccatgaccgcaccaacaggctccaag
ccaagctttcctgacggaatgttaattctcgttgaccctgagcaggctgttgagccaggtgatttctgcatagccagac
ttgggggtgatgagtttaccttcaagaaactgatcagggatagcggtcaggtgtttttacaaccactaaacccacagta
cccaatgatcccatgcaatgagagttgttccgttgtggggaaagttatcgctagtcagtggcctgaagagacgtttggc
gctgcaaacgacgaaaactacgctttagtagcttaataacgctgatagtgctagtgtagatcgctactagagccaggca
tcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactag
agtcacactggctcaccttcgggtgggcctttctgcgtttatatactagaggcaaccattatcaccgccagaggtaaaa
tagtcaacacgcacggtgttagatatttataaatagtggtgatagatttaacgttactagagattaaagaggagaaata
ctagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcac
aaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaa
aactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatat
gaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacggg
aactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgatttta
aagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaaca
aaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaa
caaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaag
atcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaact
atacaaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctg
ttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata