LOCUS       BBa_J01061              1996 bp    DNA     linear       14-May-2024
DEFINITION  [OnLock1][cI][DblTerminator][pRM GFP]
ACCESSION   BBa_J01061
VERSION     BBa_J01061.1  
FEATURES             Location/Qualifiers
     misc_feature    1..19
                     /label=TetR 1
     misc_feature    1..54
                     /label=BBa_R0040
     promoter        20..25
                     /label=-35
     misc_feature    26..44
                     /label=TetR 2
     promoter        43..48
                     /label=-10
     promoter        1..54
                     /label=BBa_R0040
     misc_feature    63..102
                     /label=BBa_J01010
     CDS             112..819
                     /label=cI lambda
     misc_feature    820..852
                     /label=LVA
     misc_feature    859..883
                     /label=Help:Barcodes
     CDS             109..858
                     /label=BBa_C0051
     stem_loop       903..946
                     /label=stem_loop
     misc_feature    892..971
                     /label=BBa_B0010
     terminator      892..971
                     /label=BBa_B0010
     stem_loop       987..1006
                     /label=T7 TE
     misc_feature    1013..1013
                     /label=stop
     polyA_site      1007..1020
                     /label=polya
     misc_feature    980..1020
                     /label=BBa_B0012
     terminator      980..1020
                     /label=BBa_B0012
     misc_feature    1037..1053
                     /label=OR1
     misc_feature    1061..1077
                     /label=OR2
     promoter        1076..1081
                     /label=-35
     misc_feature    1087..1093
                     /label=mutate to obliterate OR3
     promoter        1099..1104
                     /label=-10
     promoter        1029..1110
                     /label=BBa_I12007
     RBS             1119..1133
                     /label=RBS-1\Strong
     RBS             1126..1130
                     /label=RBS
     misc_feature    1119..1133
                     /label=BBa_B0030
     RBS             1119..1133
                     /label=BBa_B0030
     CDS             1140..1859
                     /label=GFP protein
     CDS             1140..1859
                     /label=BBa_E0040
     stem_loop       1879..1922
                     /label=stem_loop
     misc_feature    1868..1947
                     /label=BBa_B0010
     terminator      1868..1947
                     /label=BBa_B0010
     stem_loop       1963..1982
                     /label=T7 TE
     misc_feature    1989..1989
                     /label=stop
     polyA_site      1983..1996
                     /label=polya
     misc_feature    1956..1996
                     /label=BBa_B0012
     terminator      1956..1996
                     /label=BBa_B0012
ORIGIN
        1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag
       61 agaactagaa tcacctcttg gatttgggta ttaaagagga gatactagat gagcacaaaa
      121 aagaaaccat taacacaaga gcagcttgag gacgcacgtc gccttaaagc aatttatgaa
      181 aaaaagaaaa atgaacttgg cttatcccag gaatctgtcg cagacaagat ggggatgggg
      241 cagtcaggcg ttggtgcttt atttaatggc atcaatgcat taaatgctta taacgccgca
      301 ttgcttgcaa aaattctcaa agttagcgtt gaagaattta gcccttcaat cgccagagaa
      361 atctacgaga tgtatgaagc ggttagtatg cagccgtcac ttagaagtga gtatgagtac
      421 cctgtttttt ctcatgttca ggcagggatg ttctcacctg agcttagaac ctttaccaaa
      481 ggtgatgcgg agagatgggt aagcacaacc aaaaaagcca gtgattctgc attctggctt
      541 gaggttgaag gtaattccat gaccgcacca acaggctcca agccaagctt tcctgacgga
      601 atgttaattc tcgttgaccc tgagcaggct gttgagccag gtgatttctg catagccaga
      661 cttgggggtg atgagtttac cttcaagaaa ctgatcaggg atagcggtca ggtgttttta
      721 caaccactaa acccacagta cccaatgatc ccatgcaatg agagttgttc cgttgtgggg
      781 aaagttatcg ctagtcagtg gcctgaagag acgtttggcg ctgcaaacga cgaaaactac
      841 gctttagtag cttaataacg ctgatagtgc tagtgtagat cgctactaga gccaggcatc
      901 aaataaaacg aaaggctcag tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg
      961 tgaacgctct ctactagagt cacactggct caccttcggg tgggcctttc tgcgtttata
     1021 tactagaggc aaccattatc accgccagag gtaaaatagt caacacgcac ggtgttagat
     1081 atttataaat agtggtgata gatttaacgt tactagagat taaagaggag aaatactaga
     1141 tgcgtaaagg agaagaactt ttcactggag ttgtcccaat tcttgttgaa ttagatggtg
     1201 atgttaatgg gcacaaattt tctgtcagtg gagagggtga aggtgatgca acatacggaa
     1261 aacttaccct taaatttatt tgcactactg gaaaactacc tgttccatgg ccaacacttg
     1321 tcactacttt cggttatggt gttcaatgct ttgcgagata cccagatcat atgaaacagc
     1381 atgacttttt caagagtgcc atgcccgaag gttatgtaca ggaaagaact atatttttca
     1441 aagatgacgg gaactacaag acacgtgctg aagtcaagtt tgaaggtgat acccttgtta
     1501 atagaatcga gttaaaaggt attgatttta aagaagatgg aaacattctt ggacacaaat
     1561 tggaatacaa ctataactca cacaatgtat acatcatggc agacaaacaa aagaatggaa
     1621 tcaaagttaa cttcaaaatt agacacaaca ttgaagatgg aagcgttcaa ctagcagacc
     1681 attatcaaca aaatactcca attggcgatg gccctgtcct tttaccagac aaccattacc
     1741 tgtccacaca atctgccctt tcgaaagatc ccaacgaaaa gagagaccac atggtccttc
     1801 ttgagtttgt aacagctgct gggattacac atggcatgga tgaactatac aaataataat
     1861 actagagcca ggcatcaaat aaaacgaaag gctcagtcga aagactgggc ctttcgtttt
     1921 atctgttgtt tgtcggtgaa cgctctctac tagagtcaca ctggctcacc ttcgggtggg
     1981 cctttctgcg tttata
//