##gff-version 3
##sequence-region BBa_J01061 1 1996
BBa_J01061	.	CDS	1140	1859	.	+	0	ID=BBa_E0040;Name=BBa_E0040
BBa_J01061	.	CDS	1140	1859	.	+	0	ID=annotation1934520;Name=GFP protein;Parent=BBa_E0040
BBa_J01061	.	terminator	980	1020	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_J01061	.	stop_codon	1013	1013	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_J01061	.	engineered_region	980	1020	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_J01061	.	stem_loop	987	1006	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_J01061	.	polyA_site	1007	1020	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_J01061	.	CDS	109	858	.	+	0	ID=BBa_C0051;Name=BBa_C0051
BBa_J01061	.	tag	820	852	.	+	0	ID=annotation23335;Name=LVA;Parent=BBa_C0051
BBa_J01061	.	engineered_tag	859	883	.	+	0	ID=annotation2213991;Name=Help:Barcodes;Parent=BBa_C0051
BBa_J01061	.	CDS	112	819	.	+	0	ID=annotation23334;Name=cI lambda;Parent=BBa_C0051
BBa_J01061	.	terminator	1868	1947	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_J01061	.	stem_loop	1879	1922	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_J01061	.	engineered_region	1868	1947	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_J01061	.	terminator	892	971	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_J01061	.	stem_loop	903	946	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_J01061	.	engineered_region	892	971	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_J01061	.	terminator	1956	1996	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_J01061	.	stop_codon	1989	1989	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_J01061	.	engineered_region	1956	1996	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_J01061	.	stem_loop	1963	1982	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_J01061	.	polyA_site	1983	1996	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_J01061	.	promoter	1029	1110	.	+	0	ID=BBa_I12007;Name=BBa_I12007
BBa_J01061	.	non_covalent_binding_site	1061	1077	.	+	0	ID=annotation937702;Name=OR2;Parent=BBa_I12007
BBa_J01061	.	sequence_alteration	1087	1093	.	+	0	ID=annotation937704;Name=mutate to obliterate OR3;Parent=BBa_I12007
BBa_J01061	.	promoter	1099	1104	.	+	0	ID=annotation937705;Name=-10;Parent=BBa_I12007
BBa_J01061	.	promoter	1076	1081	.	+	0	ID=annotation937703;Name=-35;Parent=BBa_I12007
BBa_J01061	.	non_covalent_binding_site	1037	1053	.	+	0	ID=annotation937701;Name=OR1;Parent=BBa_I12007
BBa_J01061	.	promoter	1	54	.	+	0	ID=BBa_R0040;Name=BBa_R0040
BBa_J01061	.	promoter	20	25	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_J01061	.	non_covalent_binding_site	26	44	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_J01061	.	engineered_region	1	54	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_J01061	.	non_covalent_binding_site	1	19	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_J01061	.	promoter	43	48	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_J01061	.	ribosome_entry_site	1119	1133	.	+	0	ID=BBa_B0030;Name=BBa_B0030
BBa_J01061	.	ribosome_entry_site	1119	1133	.	+	0	ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030
BBa_J01061	.	ribosome_entry_site	1126	1130	.	+	0	ID=annotation1702;Name=RBS;Parent=BBa_B0030
BBa_J01061	.	engineered_region	1119	1133	.	+	0	ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030
BBa_J01061	.	mature_transcript_region	63	102	.	+	0	ID=BBa_J01010;Name=BBa_J01010
>BBa_J01061
tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagaactagaatcacctctt
ggatttgggtattaaagaggagatactagatgagcacaaaaaagaaaccattaacacaagagcagcttgaggacgcacg
tcgccttaaagcaatttatgaaaaaaagaaaaatgaacttggcttatcccaggaatctgtcgcagacaagatggggatg
gggcagtcaggcgttggtgctttatttaatggcatcaatgcattaaatgcttataacgccgcattgcttgcaaaaattc
tcaaagttagcgttgaagaatttagcccttcaatcgccagagaaatctacgagatgtatgaagcggttagtatgcagcc
gtcacttagaagtgagtatgagtaccctgttttttctcatgttcaggcagggatgttctcacctgagcttagaaccttt
accaaaggtgatgcggagagatgggtaagcacaaccaaaaaagccagtgattctgcattctggcttgaggttgaaggta
attccatgaccgcaccaacaggctccaagccaagctttcctgacggaatgttaattctcgttgaccctgagcaggctgt
tgagccaggtgatttctgcatagccagacttgggggtgatgagtttaccttcaagaaactgatcagggatagcggtcag
gtgtttttacaaccactaaacccacagtacccaatgatcccatgcaatgagagttgttccgttgtggggaaagttatcg
ctagtcagtggcctgaagagacgtttggcgctgcaaacgacgaaaactacgctttagtagcttaataacgctgatagtg
ctagtgtagatcgctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatct
gttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactaga
ggcaaccattatcaccgccagaggtaaaatagtcaacacgcacggtgttagatatttataaatagtggtgatagattta
acgttactagagattaaagaggagaaatactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttg
ttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaact
tacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgtt
caatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacagg
aaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaa
tagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactca
cacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatg
gaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccatta
cctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagct
gctgggattacacatggcatggatgaactatacaaataataatactagagccaggcatcaaataaaacgaaaggctcag
tcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgg
gtgggcctttctgcgtttata