LOCUS       BBa_J01062              2369 bp    DNA     linear       15-May-2024
DEFINITION  Mobilized OriTF with Lock1ed cI --> pRM --> GFP = 
            [OriTF][OnLock1][cI][DblTerminator][pRM GFP]
ACCESSION   BBa_J01062
VERSION     BBa_J01062.1  
FEATURES             Location/Qualifiers
     oriT            1..365
                     /label=BBa_J01002
     misc_feature    374..392
                     /label=TetR 1
     misc_feature    374..427
                     /label=BBa_R0040
     promoter        393..398
                     /label=-35
     misc_feature    399..417
                     /label=TetR 2
     promoter        416..421
                     /label=-10
     promoter        374..427
                     /label=BBa_R0040
     misc_feature    436..475
                     /label=BBa_J01010
     CDS             485..1192
                     /label=cI lambda
     misc_feature    1193..1225
                     /label=LVA
     misc_feature    1232..1256
                     /label=Help:Barcodes
     CDS             482..1231
                     /label=BBa_C0051
     stem_loop       1276..1319
                     /label=stem_loop
     misc_feature    1265..1344
                     /label=BBa_B0010
     terminator      1265..1344
                     /label=BBa_B0010
     stem_loop       1360..1379
                     /label=T7 TE
     misc_feature    1386..1386
                     /label=stop
     polyA_site      1380..1393
                     /label=polya
     misc_feature    1353..1393
                     /label=BBa_B0012
     terminator      1353..1393
                     /label=BBa_B0012
     misc_feature    1410..1426
                     /label=OR1
     misc_feature    1434..1450
                     /label=OR2
     promoter        1449..1454
                     /label=-35
     misc_feature    1460..1466
                     /label=mutate to obliterate OR3
     promoter        1472..1477
                     /label=-10
     promoter        1402..1483
                     /label=BBa_I12007
     RBS             1492..1506
                     /label=RBS-1\Strong
     RBS             1499..1503
                     /label=RBS
     misc_feature    1492..1506
                     /label=BBa_B0030
     RBS             1492..1506
                     /label=BBa_B0030
     CDS             1513..2232
                     /label=GFP protein
     CDS             1513..2232
                     /label=BBa_E0040
     stem_loop       2252..2295
                     /label=stem_loop
     misc_feature    2241..2320
                     /label=BBa_B0010
     terminator      2241..2320
                     /label=BBa_B0010
     stem_loop       2336..2355
                     /label=T7 TE
     misc_feature    2362..2362
                     /label=stop
     polyA_site      2356..2369
                     /label=polya
     misc_feature    2329..2369
                     /label=BBa_B0012
     terminator      2329..2369
                     /label=BBa_B0012
     misc_feature    374..2369
                     /label=BBa_J01061
ORIGIN
        1 aaggctcaac aggttggtgg ttctcaccac caaaagcacc acaccccacg caaaaacaag
       61 tttttgctga tttttcttta taaatagagt gttatgaaaa attagtttct cttactctct
      121 ttatgatatt taaaaaagcg gtgtcggcgc ggctacaaca acgcgccgac accgttttgt
      181 aggggtggta ctgactattt ttataaaaaa cattatttta tattaggggt gctgctagcg
      241 gcgcggtgtg tttttttata ggataccgct aggggcgctg ctagcggtgc gtccctgttt
      301 gcattatgaa ttttagtgtt tcgaaattaa ctttatttta tgttcaaaaa aggtaatctc
      361 taatgtacta gagtccctat cagtgataga gattgacatc cctatcagtg atagagatac
      421 tgagcactac tagagaacta gaatcacctc ttggatttgg gtattaaaga ggagatacta
      481 gatgagcaca aaaaagaaac cattaacaca agagcagctt gaggacgcac gtcgccttaa
      541 agcaatttat gaaaaaaaga aaaatgaact tggcttatcc caggaatctg tcgcagacaa
      601 gatggggatg gggcagtcag gcgttggtgc tttatttaat ggcatcaatg cattaaatgc
      661 ttataacgcc gcattgcttg caaaaattct caaagttagc gttgaagaat ttagcccttc
      721 aatcgccaga gaaatctacg agatgtatga agcggttagt atgcagccgt cacttagaag
      781 tgagtatgag taccctgttt tttctcatgt tcaggcaggg atgttctcac ctgagcttag
      841 aacctttacc aaaggtgatg cggagagatg ggtaagcaca accaaaaaag ccagtgattc
      901 tgcattctgg cttgaggttg aaggtaattc catgaccgca ccaacaggct ccaagccaag
      961 ctttcctgac ggaatgttaa ttctcgttga ccctgagcag gctgttgagc caggtgattt
     1021 ctgcatagcc agacttgggg gtgatgagtt taccttcaag aaactgatca gggatagcgg
     1081 tcaggtgttt ttacaaccac taaacccaca gtacccaatg atcccatgca atgagagttg
     1141 ttccgttgtg gggaaagtta tcgctagtca gtggcctgaa gagacgtttg gcgctgcaaa
     1201 cgacgaaaac tacgctttag tagcttaata acgctgatag tgctagtgta gatcgctact
     1261 agagccaggc atcaaataaa acgaaaggct cagtcgaaag actgggcctt tcgttttatc
     1321 tgttgtttgt cggtgaacgc tctctactag agtcacactg gctcaccttc gggtgggcct
     1381 ttctgcgttt atatactaga ggcaaccatt atcaccgcca gaggtaaaat agtcaacacg
     1441 cacggtgtta gatatttata aatagtggtg atagatttaa cgttactaga gattaaagag
     1501 gagaaatact agatgcgtaa aggagaagaa cttttcactg gagttgtccc aattcttgtt
     1561 gaattagatg gtgatgttaa tgggcacaaa ttttctgtca gtggagaggg tgaaggtgat
     1621 gcaacatacg gaaaacttac ccttaaattt atttgcacta ctggaaaact acctgttcca
     1681 tggccaacac ttgtcactac tttcggttat ggtgttcaat gctttgcgag atacccagat
     1741 catatgaaac agcatgactt tttcaagagt gccatgcccg aaggttatgt acaggaaaga
     1801 actatatttt tcaaagatga cgggaactac aagacacgtg ctgaagtcaa gtttgaaggt
     1861 gatacccttg ttaatagaat cgagttaaaa ggtattgatt ttaaagaaga tggaaacatt
     1921 cttggacaca aattggaata caactataac tcacacaatg tatacatcat ggcagacaaa
     1981 caaaagaatg gaatcaaagt taacttcaaa attagacaca acattgaaga tggaagcgtt
     2041 caactagcag accattatca acaaaatact ccaattggcg atggccctgt ccttttacca
     2101 gacaaccatt acctgtccac acaatctgcc ctttcgaaag atcccaacga aaagagagac
     2161 cacatggtcc ttcttgagtt tgtaacagct gctgggatta cacatggcat ggatgaacta
     2221 tacaaataat aatactagag ccaggcatca aataaaacga aaggctcagt cgaaagactg
     2281 ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc tactagagtc acactggctc
     2341 accttcgggt gggcctttct gcgtttata
//