##gff-version 3
##sequence-region BBa_J01062 1 2369
BBa_J01062	.	oriT	1	365	.	+	0	ID=BBa_J01002;Name=BBa_J01002
BBa_J01062	.	engineered_region	374	2369	.	+	0	ID=BBa_J01061;Name=BBa_J01061
BBa_J01062	.	promoter	374	427	.	+	0	ID=BBa_R0040;Name=BBa_R0040;Parent=BBa_J01061
BBa_J01062	.	promoter	393	398	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_J01062	.	non_covalent_binding_site	399	417	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_J01062	.	engineered_region	374	427	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_J01062	.	non_covalent_binding_site	374	392	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_J01062	.	promoter	416	421	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_J01062	.	promoter	1402	1483	.	+	0	ID=BBa_I12007;Name=BBa_I12007;Parent=BBa_J01061
BBa_J01062	.	sequence_alteration	1460	1466	.	+	0	ID=annotation937704;Name=mutate to obliterate OR3;Parent=BBa_I12007
BBa_J01062	.	promoter	1472	1477	.	+	0	ID=annotation937705;Name=-10;Parent=BBa_I12007
BBa_J01062	.	promoter	1449	1454	.	+	0	ID=annotation937703;Name=-35;Parent=BBa_I12007
BBa_J01062	.	non_covalent_binding_site	1434	1450	.	+	0	ID=annotation937702;Name=OR2;Parent=BBa_I12007
BBa_J01062	.	non_covalent_binding_site	1410	1426	.	+	0	ID=annotation937701;Name=OR1;Parent=BBa_I12007
BBa_J01062	.	terminator	1353	1393	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_J01061
BBa_J01062	.	engineered_region	1353	1393	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_J01062	.	stop_codon	1386	1386	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_J01062	.	stem_loop	1360	1379	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_J01062	.	polyA_site	1380	1393	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_J01062	.	terminator	1265	1344	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_J01061
BBa_J01062	.	stem_loop	1276	1319	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_J01062	.	engineered_region	1265	1344	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_J01062	.	mature_transcript_region	436	475	.	+	0	ID=BBa_J01010;Name=BBa_J01010;Parent=BBa_J01061
BBa_J01062	.	CDS	482	1231	.	+	0	ID=BBa_C0051;Name=BBa_C0051;Parent=BBa_J01061
BBa_J01062	.	tag	1193	1225	.	+	0	ID=annotation23335;Name=LVA;Parent=BBa_C0051
BBa_J01062	.	CDS	485	1192	.	+	0	ID=annotation23334;Name=cI lambda;Parent=BBa_C0051
BBa_J01062	.	engineered_tag	1232	1256	.	+	0	ID=annotation2213991;Name=Help:Barcodes;Parent=BBa_C0051
BBa_J01062	.	terminator	2329	2369	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_J01061
BBa_J01062	.	engineered_region	2329	2369	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_J01062	.	stop_codon	2362	2362	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_J01062	.	stem_loop	2336	2355	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_J01062	.	polyA_site	2356	2369	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_J01062	.	terminator	2241	2320	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_J01061
BBa_J01062	.	stem_loop	2252	2295	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_J01062	.	engineered_region	2241	2320	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_J01062	.	CDS	1513	2232	.	+	0	ID=BBa_E0040;Name=BBa_E0040;Parent=BBa_J01061
BBa_J01062	.	CDS	1513	2232	.	+	0	ID=annotation1934520;Name=GFP protein;Parent=BBa_E0040
BBa_J01062	.	ribosome_entry_site	1492	1506	.	+	0	ID=BBa_B0030;Name=BBa_B0030;Parent=BBa_J01061
BBa_J01062	.	engineered_region	1492	1506	.	+	0	ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030
BBa_J01062	.	ribosome_entry_site	1499	1503	.	+	0	ID=annotation1702;Name=RBS;Parent=BBa_B0030
BBa_J01062	.	ribosome_entry_site	1492	1506	.	+	0	ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030
>BBa_J01062
aaggctcaacaggttggtggttctcaccaccaaaagcaccacaccccacgcaaaaacaagtttttgctgatttttcttt
ataaatagagtgttatgaaaaattagtttctcttactctctttatgatatttaaaaaagcggtgtcggcgcggctacaa
caacgcgccgacaccgttttgtaggggtggtactgactatttttataaaaaacattattttatattaggggtgctgcta
gcggcgcggtgtgtttttttataggataccgctaggggcgctgctagcggtgcgtccctgtttgcattatgaattttag
tgtttcgaaattaactttattttatgttcaaaaaaggtaatctctaatgtactagagtccctatcagtgatagagattg
acatccctatcagtgatagagatactgagcactactagagaactagaatcacctcttggatttgggtattaaagaggag
atactagatgagcacaaaaaagaaaccattaacacaagagcagcttgaggacgcacgtcgccttaaagcaatttatgaa
aaaaagaaaaatgaacttggcttatcccaggaatctgtcgcagacaagatggggatggggcagtcaggcgttggtgctt
tatttaatggcatcaatgcattaaatgcttataacgccgcattgcttgcaaaaattctcaaagttagcgttgaagaatt
tagcccttcaatcgccagagaaatctacgagatgtatgaagcggttagtatgcagccgtcacttagaagtgagtatgag
taccctgttttttctcatgttcaggcagggatgttctcacctgagcttagaacctttaccaaaggtgatgcggagagat
gggtaagcacaaccaaaaaagccagtgattctgcattctggcttgaggttgaaggtaattccatgaccgcaccaacagg
ctccaagccaagctttcctgacggaatgttaattctcgttgaccctgagcaggctgttgagccaggtgatttctgcata
gccagacttgggggtgatgagtttaccttcaagaaactgatcagggatagcggtcaggtgtttttacaaccactaaacc
cacagtacccaatgatcccatgcaatgagagttgttccgttgtggggaaagttatcgctagtcagtggcctgaagagac
gtttggcgctgcaaacgacgaaaactacgctttagtagcttaataacgctgatagtgctagtgtagatcgctactagag
ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctct
ctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagaggcaaccattatcaccgccaga
ggtaaaatagtcaacacgcacggtgttagatatttataaatagtggtgatagatttaacgttactagagattaaagagg
agaaatactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaa
tgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcact
actggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccag
atcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaaga
tgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtatt
gattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcag
acaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagacca
ttatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctt
tcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatgg
atgaactatacaaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgtt
ttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata