LOCUS       BBa_J01064              1296 bp    DNA     linear       14-May-2024
DEFINITION  TEST F mobilizable plasmid with RFP reporter = [OriTF][OnRFP]
ACCESSION   BBa_J01064
VERSION     BBa_J01064.1  
FEATURES             Location/Qualifiers
     oriT            1..365
                     /label=BBa_J01002
     misc_feature    374..392
                     /label=TetR 1
     misc_feature    374..427
                     /label=BBa_R0040
     promoter        393..398
                     /label=-35
     misc_feature    399..417
                     /label=TetR 2
     promoter        416..421
                     /label=-10
     promoter        374..427
                     /label=BBa_R0040
     misc_feature    440..443
                     /label=conserved
     RBS             436..447
                     /label=BBa_B0034
     CDS             454..1128
                     /label=mrfp1
     misc_feature    1135..1159
                     /label=Help:Barcodes
     CDS             454..1134
                     /label=BBa_E1010
     stem_loop       1179..1222
                     /label=stem_loop
     misc_feature    1168..1247
                     /label=BBa_B0010
     terminator      1168..1247
                     /label=BBa_B0010
     stem_loop       1263..1282
                     /label=T7 TE
     misc_feature    1289..1289
                     /label=stop
     polyA_site      1283..1296
                     /label=polya
     misc_feature    1256..1296
                     /label=BBa_B0012
     terminator      1256..1296
                     /label=BBa_B0012
     misc_feature    374..1296
                     /label=BBa_I13521
ORIGIN
        1 aaggctcaac aggttggtgg ttctcaccac caaaagcacc acaccccacg caaaaacaag
       61 tttttgctga tttttcttta taaatagagt gttatgaaaa attagtttct cttactctct
      121 ttatgatatt taaaaaagcg gtgtcggcgc ggctacaaca acgcgccgac accgttttgt
      181 aggggtggta ctgactattt ttataaaaaa cattatttta tattaggggt gctgctagcg
      241 gcgcggtgtg tttttttata ggataccgct aggggcgctg ctagcggtgc gtccctgttt
      301 gcattatgaa ttttagtgtt tcgaaattaa ctttatttta tgttcaaaaa aggtaatctc
      361 taatgtacta gagtccctat cagtgataga gattgacatc cctatcagtg atagagatac
      421 tgagcactac tagagaaaga ggagaaatac tagatggctt cctccgaaga cgttatcaaa
      481 gagttcatgc gtttcaaagt tcgtatggaa ggttccgtta acggtcacga gttcgaaatc
      541 gaaggtgaag gtgaaggtcg tccgtacgaa ggtacccaga ccgctaaact gaaagttacc
      601 aaaggtggtc cgctgccgtt cgcttgggac atcctgtccc cgcagttcca gtacggttcc
      661 aaagcttacg ttaaacaccc ggctgacatc ccggactacc tgaaactgtc cttcccggaa
      721 ggtttcaaat gggaacgtgt tatgaacttc gaagacggtg gtgttgttac cgttacccag
      781 gactcctccc tgcaagacgg tgagttcatc tacaaagtta aactgcgtgg taccaacttc
      841 ccgtccgacg gtccggttat gcagaaaaaa accatgggtt gggaagcttc caccgaacgt
      901 atgtacccgg aagacggtgc tctgaaaggt gaaatcaaaa tgcgtctgaa actgaaagac
      961 ggtggtcact acgacgctga agttaaaacc acctacatgg ctaaaaaacc ggttcagctg
     1021 ccgggtgctt acaaaaccga catcaaactg gacatcacct cccacaacga agactacacc
     1081 atcgttgaac agtacgaacg tgctgaaggt cgtcactcca ccggtgctta ataacgctga
     1141 tagtgctagt gtagatcgct actagagcca ggcatcaaat aaaacgaaag gctcagtcga
     1201 aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa cgctctctac tagagtcaca
     1261 ctggctcacc ttcgggtggg cctttctgcg tttata
//