##gff-version 3
##sequence-region BBa_J01064 1 1296
BBa_J01064	.	oriT	1	365	.	+	0	ID=BBa_J01002;Name=BBa_J01002
BBa_J01064	.	engineered_region	374	1296	.	+	0	ID=BBa_I13521;Name=BBa_I13521
BBa_J01064	.	promoter	374	427	.	+	0	ID=BBa_R0040;Name=BBa_R0040;Parent=BBa_I13521
BBa_J01064	.	promoter	416	421	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_J01064	.	promoter	393	398	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_J01064	.	engineered_region	374	427	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_J01064	.	non_covalent_binding_site	399	417	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_J01064	.	non_covalent_binding_site	374	392	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_J01064	.	terminator	1168	1247	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_I13521
BBa_J01064	.	engineered_region	1168	1247	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_J01064	.	stem_loop	1179	1222	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_J01064	.	ribosome_entry_site	436	447	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_I13521
BBa_J01064	.	conserved_region	440	443	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_J01064	.	terminator	1256	1296	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_I13521
BBa_J01064	.	engineered_region	1256	1296	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_J01064	.	stem_loop	1263	1282	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_J01064	.	stop_codon	1289	1289	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_J01064	.	polyA_site	1283	1296	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_J01064	.	CDS	454	1134	.	+	0	ID=BBa_E1010;Name=BBa_E1010;Parent=BBa_I13521
BBa_J01064	.	CDS	454	1128	.	+	0	ID=annotation1014044;Name=mrfp1;Parent=BBa_E1010
BBa_J01064	.	engineered_tag	1135	1159	.	+	0	ID=annotation2214014;Name=Help:Barcodes;Parent=BBa_E1010
>BBa_J01064
aaggctcaacaggttggtggttctcaccaccaaaagcaccacaccccacgcaaaaacaagtttttgctgatttttcttt
ataaatagagtgttatgaaaaattagtttctcttactctctttatgatatttaaaaaagcggtgtcggcgcggctacaa
caacgcgccgacaccgttttgtaggggtggtactgactatttttataaaaaacattattttatattaggggtgctgcta
gcggcgcggtgtgtttttttataggataccgctaggggcgctgctagcggtgcgtccctgtttgcattatgaattttag
tgtttcgaaattaactttattttatgttcaaaaaaggtaatctctaatgtactagagtccctatcagtgatagagattg
acatccctatcagtgatagagatactgagcactactagagaaagaggagaaatactagatggcttcctccgaagacgtt
atcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtg
aaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacat
cctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtcc
ttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccc
tgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaa
aaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctg
aaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtg
cttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctga
aggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgctactagagccaggcatcaaataaaac
gaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactgg
ctcaccttcgggtgggcctttctgcgtttata