LOCUS       BBa_J01071              2111 bp    DNA     linear       13-May-2024
DEFINITION  TEST [pBad/araC][Key2][pTet][Lock1][RFP]
ACCESSION   BBa_J01071
VERSION     BBa_J01071.1  
FEATURES             Location/Qualifiers
     promoter        1080..1210
                     /label=Pbad
     promoter        1043..1114
                     /label=PC
     misc_feature    1..1079
                     /label=AraC
     promoter        1..1210
                     /label=BBa_I0500
     misc_feature    1219..1289
                     /label=BBa_J01009
     misc_feature    1298..1316
                     /label=TetR 1
     misc_feature    1298..1351
                     /label=BBa_R0040
     promoter        1317..1322
                     /label=-35
     misc_feature    1323..1341
                     /label=TetR 2
     promoter        1340..1345
                     /label=-10
     promoter        1298..1351
                     /label=BBa_R0040
     misc_feature    1360..1399
                     /label=BBa_J01010
     CDS             1406..2080
                     /label=mrfp1
     misc_feature    2087..2111
                     /label=Help:Barcodes
     CDS             1406..2086
                     /label=BBa_E1010
ORIGIN
        1 ttatgacaac ttgacggcta catcattcac tttttcttca caaccggcac ggaactcgct
       61 cgggctggcc ccggtgcatt ttttaaatac ccgcgagaaa tagagttgat cgtcaaaacc
      121 aacattgcga ccgacggtgg cgataggcat ccgggtggtg ctcaaaagca gcttcgcctg
      181 gctgatacgt tggtcctcgc gccagcttaa gacgctaatc cctaactgct ggcggaaaag
      241 atgtgacaga cgcgacggcg acaagcaaac atgctgtgcg acgctggcga tatcaaaatt
      301 gctgtctgcc aggtgatcgc tgatgtactg acaagcctcg cgtacccgat tatccatcgg
      361 tggatggagc gactcgttaa tcgcttccat gcgccgcagt aacaattgct caagcagatt
      421 tatcgccagc agctccgaat agcgcccttc cccttgcccg gcgttaatga tttgcccaaa
      481 caggtcgctg aaatgcggct ggtgcgcttc atccgggcga aagaaccccg tattggcaaa
      541 tattgacggc cagttaagcc attcatgcca gtaggcgcgc ggacgaaagt aaacccactg
      601 gtgataccat tcgcgagcct ccggatgacg accgtagtga tgaatctctc ctggcgggaa
      661 cagcaaaata tcacccggtc ggcaaacaaa ttctcgtccc tgatttttca ccaccccctg
      721 accgcgaatg gtgagattga gaatataacc tttcattccc agcggtcggt cgataaaaaa
      781 atcgagataa ccgttggcct caatcggcgt taaacccgcc accagatggg cattaaacga
      841 gtatcccggc agcaggggat cattttgcgc ttcagccata cttttcatac tcccgccatt
      901 cagagaagaa accaattgtc catattgcat cagacattgc cgtcactgcg tcttttactg
      961 gctcttctcg ctaaccaaac cggtaacccc gcttattaaa agcattctgt aacaaagcgg
     1021 gaccaaagcc atgacaaaaa cgcgtaacaa aagtgtctat aatcacggca gaaaagtcca
     1081 cattgattat ttgcacggcg tcacactttg ctatgccata gcatttttat ccataagatt
     1141 agcggatcct acctgacgct ttttatcgca actctctact gtttctccat acccgttttt
     1201 ttgggctagc tactagagac ccaatcactg gaggtgaatc tagtaggtgg ttaatgaaaa
     1261 ttaacttact tactagaaat atctcttgat actagagtcc ctatcagtga tagagattga
     1321 catccctatc agtgatagag atactgagca ctactagaga actagaatca cctcttggat
     1381 ttgggtatta aagaggagat actagatggc ttcctccgaa gacgttatca aagagttcat
     1441 gcgtttcaaa gttcgtatgg aaggttccgt taacggtcac gagttcgaaa tcgaaggtga
     1501 aggtgaaggt cgtccgtacg aaggtaccca gaccgctaaa ctgaaagtta ccaaaggtgg
     1561 tccgctgccg ttcgcttggg acatcctgtc cccgcagttc cagtacggtt ccaaagctta
     1621 cgttaaacac ccggctgaca tcccggacta cctgaaactg tccttcccgg aaggtttcaa
     1681 atgggaacgt gttatgaact tcgaagacgg tggtgttgtt accgttaccc aggactcctc
     1741 cctgcaagac ggtgagttca tctacaaagt taaactgcgt ggtaccaact tcccgtccga
     1801 cggtccggtt atgcagaaaa aaaccatggg ttgggaagct tccaccgaac gtatgtaccc
     1861 ggaagacggt gctctgaaag gtgaaatcaa aatgcgtctg aaactgaaag acggtggtca
     1921 ctacgacgct gaagttaaaa ccacctacat ggctaaaaaa ccggttcagc tgccgggtgc
     1981 ttacaaaacc gacatcaaac tggacatcac ctcccacaac gaagactaca ccatcgttga
     2041 acagtacgaa cgtgctgaag gtcgtcactc caccggtgct taataacgct gatagtgcta
     2101 gtgtagatcg c
//