##gff-version 3
##sequence-region BBa_J01074 1 1297
BBa_J01074	.	oriT	1	371	.	+	0	ID=BBa_J01003;Name=BBa_J01003
BBa_J01074	.	ribosome_entry_site	437	448	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_J01074	.	conserved_region	441	444	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_J01074	.	CDS	455	1135	.	+	0	ID=BBa_E1010;Name=BBa_E1010
BBa_J01074	.	CDS	455	1129	.	+	0	ID=annotation1014044;Name=mrfp1;Parent=BBa_E1010
BBa_J01074	.	engineered_tag	1136	1160	.	+	0	ID=annotation2214014;Name=Help:Barcodes;Parent=BBa_E1010
BBa_J01074	.	terminator	1257	1297	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_J01074	.	stem_loop	1264	1283	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_J01074	.	polyA_site	1284	1297	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_J01074	.	stop_codon	1290	1290	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_J01074	.	engineered_region	1257	1297	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_J01074	.	promoter	380	428	.	+	0	ID=BBa_R0051;Name=BBa_R0051
BBa_J01074	.	engineered_region	380	428	.	+	0	ID=annotation7067;Name=BBa_R0051;Parent=BBa_R0051
BBa_J01074	.	promoter	417	422	.	+	0	ID=annotation2022;Name=-10;Parent=BBa_R0051
BBa_J01074	.	non_covalent_binding_site	404	420	.	+	0	ID=annotation2024;Name=OR1;Parent=BBa_R0051
BBa_J01074	.	promoter	394	399	.	+	0	ID=annotation2023;Name=-35;Parent=BBa_R0051
BBa_J01074	.	non_covalent_binding_site	380	396	.	+	0	ID=annotation2025;Name=OR2;Parent=BBa_R0051
BBa_J01074	.	terminator	1169	1248	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_J01074	.	stem_loop	1180	1223	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_J01074	.	engineered_region	1169	1248	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
>BBa_J01074
gccgccttttcctcaatcgctcttcgttcgtctggaaggcagtacaccttgataggtgggctgcccttcctggttggct
tggtttcatcagccatccgcttgccctcatctgttacgccggcggtagccggccagcctcgcagagcaggattcccgtt
gagcaccgccaggtgcgaataagggacagtgaagaaggaacacccgctcgcgggtgggcctacttcacctatcctgccc
ggctgacgccgttggatacaccaaggaaagtctacacgaaccctttggcaaaatcctgtatatcgtgcgaaaaaggatg
gatataccgaaaaaatcgctataatgaccccgaagcagggttatgcagcggaaaatactagagtaacaccgtgcgtgtt
gactattttacctctggcggtgataatggttgctactagagaaagaggagaaatactagatggcttcctccgaagacgt
tatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggt
gaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggaca
tcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtc
cttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctcc
ctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaa
aaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtct
gaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggt
gcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctg
aaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgctactagagccaggcatcaaataaaa
cgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactg
gctcaccttcgggtgggcctttctgcgtttata