LOCUS       BBa_J01075               972 bp    DNA     linear       26-Dec-2024
DEFINITION  TEST [pTet][cI][DblTerminator]
ACCESSION   BBa_J01075
VERSION     BBa_J01075.1  
FEATURES             Location/Qualifiers
     misc_feature    1..19
                     /label=TetR 1
     misc_feature    1..54
                     /label=BBa_R0040
     promoter        20..25
                     /label=-35
     misc_feature    26..44
                     /label=TetR 2
     promoter        43..48
                     /label=-10
     promoter        1..54
                     /label=BBa_R0040
     CDS             64..771
                     /label=cI lambda
     misc_feature    772..804
                     /label=LVA
     misc_feature    811..835
                     /label=Help:Barcodes
     CDS             61..810
                     /label=BBa_C0051
     stem_loop       855..898
                     /label=stem_loop
     misc_feature    844..923
                     /label=BBa_B0010
     terminator      844..923
                     /label=BBa_B0010
     stem_loop       939..958
                     /label=T7 TE
     misc_feature    965..965
                     /label=stop
     polyA_site      959..972
                     /label=polya
     misc_feature    932..972
                     /label=BBa_B0012
     terminator      932..972
                     /label=BBa_B0012
ORIGIN
        1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag
       61 atgagcacaa aaaagaaacc attaacacaa gagcagcttg aggacgcacg tcgccttaaa
      121 gcaatttatg aaaaaaagaa aaatgaactt ggcttatccc aggaatctgt cgcagacaag
      181 atggggatgg ggcagtcagg cgttggtgct ttatttaatg gcatcaatgc attaaatgct
      241 tataacgccg cattgcttgc aaaaattctc aaagttagcg ttgaagaatt tagcccttca
      301 atcgccagag aaatctacga gatgtatgaa gcggttagta tgcagccgtc acttagaagt
      361 gagtatgagt accctgtttt ttctcatgtt caggcaggga tgttctcacc tgagcttaga
      421 acctttacca aaggtgatgc ggagagatgg gtaagcacaa ccaaaaaagc cagtgattct
      481 gcattctggc ttgaggttga aggtaattcc atgaccgcac caacaggctc caagccaagc
      541 tttcctgacg gaatgttaat tctcgttgac cctgagcagg ctgttgagcc aggtgatttc
      601 tgcatagcca gacttggggg tgatgagttt accttcaaga aactgatcag ggatagcggt
      661 caggtgtttt tacaaccact aaacccacag tacccaatga tcccatgcaa tgagagttgt
      721 tccgttgtgg ggaaagttat cgctagtcag tggcctgaag agacgtttgg cgctgcaaac
      781 gacgaaaact acgctttagt agcttaataa cgctgatagt gctagtgtag atcgctacta
      841 gagccaggca tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt cgttttatct
      901 gttgtttgtc ggtgaacgct ctctactaga gtcacactgg ctcaccttcg ggtgggcctt
      961 tctgcgttta ta
//