LOCUS       BBa_J01079              1948 bp    DNA     linear       29-Nov-2024
DEFINITION  TEST [pTet][cI][DblTerminator][pRM GFP]
ACCESSION   BBa_J01079
VERSION     BBa_J01079.1  
FEATURES             Location/Qualifiers
     misc_feature    1..19
                     /label=TetR 1
     misc_feature    1..54
                     /label=BBa_R0040
     promoter        20..25
                     /label=-35
     misc_feature    26..44
                     /label=TetR 2
     promoter        43..48
                     /label=-10
     promoter        1..54
                     /label=BBa_R0040
     CDS             64..771
                     /label=cI lambda
     misc_feature    772..804
                     /label=LVA
     misc_feature    811..835
                     /label=Help:Barcodes
     CDS             61..810
                     /label=BBa_C0051
     stem_loop       855..898
                     /label=stem_loop
     misc_feature    844..923
                     /label=BBa_B0010
     terminator      844..923
                     /label=BBa_B0010
     stem_loop       939..958
                     /label=T7 TE
     misc_feature    965..965
                     /label=stop
     polyA_site      959..972
                     /label=polya
     misc_feature    932..972
                     /label=BBa_B0012
     terminator      932..972
                     /label=BBa_B0012
     misc_feature    989..1005
                     /label=OR1
     misc_feature    1013..1029
                     /label=OR2
     promoter        1028..1033
                     /label=-35
     misc_feature    1039..1045
                     /label=mutate to obliterate OR3
     promoter        1051..1056
                     /label=-10
     promoter        981..1062
                     /label=BBa_I12007
     RBS             1071..1085
                     /label=RBS-1\Strong
     RBS             1078..1082
                     /label=RBS
     misc_feature    1071..1085
                     /label=BBa_B0030
     RBS             1071..1085
                     /label=BBa_B0030
     CDS             1092..1811
                     /label=GFP protein
     CDS             1092..1811
                     /label=BBa_E0040
     stem_loop       1831..1874
                     /label=stem_loop
     misc_feature    1820..1899
                     /label=BBa_B0010
     terminator      1820..1899
                     /label=BBa_B0010
     stem_loop       1915..1934
                     /label=T7 TE
     misc_feature    1941..1941
                     /label=stop
     polyA_site      1935..1948
                     /label=polya
     misc_feature    1908..1948
                     /label=BBa_B0012
     terminator      1908..1948
                     /label=BBa_B0012
ORIGIN
        1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag
       61 atgagcacaa aaaagaaacc attaacacaa gagcagcttg aggacgcacg tcgccttaaa
      121 gcaatttatg aaaaaaagaa aaatgaactt ggcttatccc aggaatctgt cgcagacaag
      181 atggggatgg ggcagtcagg cgttggtgct ttatttaatg gcatcaatgc attaaatgct
      241 tataacgccg cattgcttgc aaaaattctc aaagttagcg ttgaagaatt tagcccttca
      301 atcgccagag aaatctacga gatgtatgaa gcggttagta tgcagccgtc acttagaagt
      361 gagtatgagt accctgtttt ttctcatgtt caggcaggga tgttctcacc tgagcttaga
      421 acctttacca aaggtgatgc ggagagatgg gtaagcacaa ccaaaaaagc cagtgattct
      481 gcattctggc ttgaggttga aggtaattcc atgaccgcac caacaggctc caagccaagc
      541 tttcctgacg gaatgttaat tctcgttgac cctgagcagg ctgttgagcc aggtgatttc
      601 tgcatagcca gacttggggg tgatgagttt accttcaaga aactgatcag ggatagcggt
      661 caggtgtttt tacaaccact aaacccacag tacccaatga tcccatgcaa tgagagttgt
      721 tccgttgtgg ggaaagttat cgctagtcag tggcctgaag agacgtttgg cgctgcaaac
      781 gacgaaaact acgctttagt agcttaataa cgctgatagt gctagtgtag atcgctacta
      841 gagccaggca tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt cgttttatct
      901 gttgtttgtc ggtgaacgct ctctactaga gtcacactgg ctcaccttcg ggtgggcctt
      961 tctgcgttta tatactagag gcaaccatta tcaccgccag aggtaaaata gtcaacacgc
     1021 acggtgttag atatttataa atagtggtga tagatttaac gttactagag attaaagagg
     1081 agaaatacta gatgcgtaaa ggagaagaac ttttcactgg agttgtccca attcttgttg
     1141 aattagatgg tgatgttaat gggcacaaat tttctgtcag tggagagggt gaaggtgatg
     1201 caacatacgg aaaacttacc cttaaattta tttgcactac tggaaaacta cctgttccat
     1261 ggccaacact tgtcactact ttcggttatg gtgttcaatg ctttgcgaga tacccagatc
     1321 atatgaaaca gcatgacttt ttcaagagtg ccatgcccga aggttatgta caggaaagaa
     1381 ctatattttt caaagatgac gggaactaca agacacgtgc tgaagtcaag tttgaaggtg
     1441 atacccttgt taatagaatc gagttaaaag gtattgattt taaagaagat ggaaacattc
     1501 ttggacacaa attggaatac aactataact cacacaatgt atacatcatg gcagacaaac
     1561 aaaagaatgg aatcaaagtt aacttcaaaa ttagacacaa cattgaagat ggaagcgttc
     1621 aactagcaga ccattatcaa caaaatactc caattggcga tggccctgtc cttttaccag
     1681 acaaccatta cctgtccaca caatctgccc tttcgaaaga tcccaacgaa aagagagacc
     1741 acatggtcct tcttgagttt gtaacagctg ctgggattac acatggcatg gatgaactat
     1801 acaaataata atactagagc caggcatcaa ataaaacgaa aggctcagtc gaaagactgg
     1861 gcctttcgtt ttatctgttg tttgtcggtg aacgctctct actagagtca cactggctca
     1921 ccttcgggtg ggcctttctg cgtttata
//