LOCUS       BBa_J01097              1308 bp    DNA     linear       22-Dec-2024
DEFINITION  TEST [OriTF][pLam cI -][str RBS][GFP][dbl Terminator]
ACCESSION   BBa_J01097
VERSION     BBa_J01097.1  
FEATURES             Location/Qualifiers
     oriT            1..365
                     /label=BBa_J01002
     promoter        411..416
                     /label=-10
     promoter        388..393
                     /label=-35
     misc_feature    398..414
                     /label=OR1
     misc_feature    374..390
                     /label=OR2
     misc_feature    374..422
                     /label=BBa_R0051
     promoter        374..422
                     /label=BBa_R0051
     RBS             431..445
                     /label=RBS-1\Strong
     RBS             438..442
                     /label=RBS
     misc_feature    431..445
                     /label=BBa_B0030
     RBS             431..445
                     /label=BBa_B0030
     CDS             452..1171
                     /label=GFP protein
     CDS             452..1171
                     /label=BBa_E0040
     stem_loop       1191..1234
                     /label=stem_loop
     misc_feature    1180..1259
                     /label=BBa_B0010
     terminator      1180..1259
                     /label=BBa_B0010
     stem_loop       1275..1294
                     /label=T7 TE
     misc_feature    1301..1301
                     /label=stop
     polyA_site      1295..1308
                     /label=polya
     misc_feature    1268..1308
                     /label=BBa_B0012
     terminator      1268..1308
                     /label=BBa_B0012
     misc_feature    374..1308
                     /label=BBa_J01094
ORIGIN
        1 aaggctcaac aggttggtgg ttctcaccac caaaagcacc acaccccacg caaaaacaag
       61 tttttgctga tttttcttta taaatagagt gttatgaaaa attagtttct cttactctct
      121 ttatgatatt taaaaaagcg gtgtcggcgc ggctacaaca acgcgccgac accgttttgt
      181 aggggtggta ctgactattt ttataaaaaa cattatttta tattaggggt gctgctagcg
      241 gcgcggtgtg tttttttata ggataccgct aggggcgctg ctagcggtgc gtccctgttt
      301 gcattatgaa ttttagtgtt tcgaaattaa ctttatttta tgttcaaaaa aggtaatctc
      361 taatgtacta gagtaacacc gtgcgtgttg actattttac ctctggcggt gataatggtt
      421 gctactagag attaaagagg agaaatacta gatgcgtaaa ggagaagaac ttttcactgg
      481 agttgtccca attcttgttg aattagatgg tgatgttaat gggcacaaat tttctgtcag
      541 tggagagggt gaaggtgatg caacatacgg aaaacttacc cttaaattta tttgcactac
      601 tggaaaacta cctgttccat ggccaacact tgtcactact ttcggttatg gtgttcaatg
      661 ctttgcgaga tacccagatc atatgaaaca gcatgacttt ttcaagagtg ccatgcccga
      721 aggttatgta caggaaagaa ctatattttt caaagatgac gggaactaca agacacgtgc
      781 tgaagtcaag tttgaaggtg atacccttgt taatagaatc gagttaaaag gtattgattt
      841 taaagaagat ggaaacattc ttggacacaa attggaatac aactataact cacacaatgt
      901 atacatcatg gcagacaaac aaaagaatgg aatcaaagtt aacttcaaaa ttagacacaa
      961 cattgaagat ggaagcgttc aactagcaga ccattatcaa caaaatactc caattggcga
     1021 tggccctgtc cttttaccag acaaccatta cctgtccaca caatctgccc tttcgaaaga
     1081 tcccaacgaa aagagagacc acatggtcct tcttgagttt gtaacagctg ctgggattac
     1141 acatggcatg gatgaactat acaaataata atactagagc caggcatcaa ataaaacgaa
     1201 aggctcagtc gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctct
     1261 actagagtca cactggctca ccttcgggtg ggcctttctg cgtttata
//