LOCUS       BBa_J01099              3043 bp    DNA     linear       27-Mar-2025
DEFINITION  TEST [pBAD/araC][TraJF][DblTerm][pTet][cI][DblTerm]
ACCESSION   BBa_J01099
VERSION     BBa_J01099.1  
FEATURES             Location/Qualifiers
     promoter        1080..1210
                     /label=Pbad
     promoter        1043..1114
                     /label=PC
     misc_feature    1..1079
                     /label=AraC
     promoter        1..1210
                     /label=BBa_I0500
     misc_feature    1223..1226
                     /label=conserved
     RBS             1219..1230
                     /label=BBa_B0034
     CDS             1237..1926
                     /label=BBa_J01000
     stem_loop       1946..1989
                     /label=stem_loop
     misc_feature    1935..2014
                     /label=BBa_B0010
     terminator      1935..2014
                     /label=BBa_B0010
     stem_loop       2030..2049
                     /label=T7 TE
     misc_feature    2056..2056
                     /label=stop
     polyA_site      2050..2063
                     /label=polya
     misc_feature    2023..2063
                     /label=BBa_B0012
     terminator      2023..2063
                     /label=BBa_B0012
     misc_feature    2072..2090
                     /label=TetR 1
     misc_feature    2072..2125
                     /label=BBa_R0040
     promoter        2091..2096
                     /label=-35
     misc_feature    2097..2115
                     /label=TetR 2
     promoter        2114..2119
                     /label=-10
     promoter        2072..2125
                     /label=BBa_R0040
     CDS             2135..2842
                     /label=cI lambda
     misc_feature    2843..2875
                     /label=LVA
     misc_feature    2882..2906
                     /label=Help:Barcodes
     CDS             2132..2881
                     /label=BBa_C0051
     stem_loop       2926..2969
                     /label=stem_loop
     misc_feature    2915..2994
                     /label=BBa_B0010
     terminator      2915..2994
                     /label=BBa_B0010
     stem_loop       3010..3029
                     /label=T7 TE
     misc_feature    3036..3036
                     /label=stop
     polyA_site      3030..3043
                     /label=polya
     misc_feature    3003..3043
                     /label=BBa_B0012
     terminator      3003..3043
                     /label=BBa_B0012
ORIGIN
        1 ttatgacaac ttgacggcta catcattcac tttttcttca caaccggcac ggaactcgct
       61 cgggctggcc ccggtgcatt ttttaaatac ccgcgagaaa tagagttgat cgtcaaaacc
      121 aacattgcga ccgacggtgg cgataggcat ccgggtggtg ctcaaaagca gcttcgcctg
      181 gctgatacgt tggtcctcgc gccagcttaa gacgctaatc cctaactgct ggcggaaaag
      241 atgtgacaga cgcgacggcg acaagcaaac atgctgtgcg acgctggcga tatcaaaatt
      301 gctgtctgcc aggtgatcgc tgatgtactg acaagcctcg cgtacccgat tatccatcgg
      361 tggatggagc gactcgttaa tcgcttccat gcgccgcagt aacaattgct caagcagatt
      421 tatcgccagc agctccgaat agcgcccttc cccttgcccg gcgttaatga tttgcccaaa
      481 caggtcgctg aaatgcggct ggtgcgcttc atccgggcga aagaaccccg tattggcaaa
      541 tattgacggc cagttaagcc attcatgcca gtaggcgcgc ggacgaaagt aaacccactg
      601 gtgataccat tcgcgagcct ccggatgacg accgtagtga tgaatctctc ctggcgggaa
      661 cagcaaaata tcacccggtc ggcaaacaaa ttctcgtccc tgatttttca ccaccccctg
      721 accgcgaatg gtgagattga gaatataacc tttcattccc agcggtcggt cgataaaaaa
      781 atcgagataa ccgttggcct caatcggcgt taaacccgcc accagatggg cattaaacga
      841 gtatcccggc agcaggggat cattttgcgc ttcagccata cttttcatac tcccgccatt
      901 cagagaagaa accaattgtc catattgcat cagacattgc cgtcactgcg tcttttactg
      961 gctcttctcg ctaaccaaac cggtaacccc gcttattaaa agcattctgt aacaaagcgg
     1021 gaccaaagcc atgacaaaaa cgcgtaacaa aagtgtctat aatcacggca gaaaagtcca
     1081 cattgattat ttgcacggcg tcacactttg ctatgccata gcatttttat ccataagatt
     1141 agcggatcct acctgacgct ttttatcgca actctctact gtttctccat acccgttttt
     1201 ttgggctagc tactagagaa agaggagaaa tactagatgt atccgatgga tcgtattcaa
     1261 caaaaacatg ctcgtcaaat agatctgctg gaaaatctga cggcagttat tcaggattat
     1321 ccaaatccag cctgtatcag ggacgaaact ggaaaattta ttttttgcaa tacgctgttt
     1381 catgagtcat ttcttacaca agatcaaagt gctgaaaaat ggcttctgtc gcagagagat
     1441 ttttgtgaat tgatctctgt cacagagatg gaagcatata ggaatgagca tacgcatctt
     1501 aatcttgtag aggatgtttt tattcagaat agattctgga caatatctgt ccagtcattt
     1561 cttaatggac acagaaatat tattctgtgg caattttatg atgctgctca tgttcgtcat
     1621 aaagacagtt ataatcaaaa aacgattgtc agtgatgata tcagaaatat aatcagaaga
     1681 atgagtgatg attcttctgt atcatcatat gtaaatgatg tcttttactt atatagcacc
     1741 ggaatcagtc ataatgctat agcaagaata ttaaatatat ccatctccac atcaaagaaa
     1801 cacgcatctc tgatatgcga ctacttctct gtttctaata aagatgagtt aattatctta
     1861 ctctacaata aaaagtttat ttattattta tacgagaagg ctatgtgtat cataaatacg
     1921 cgttaatact agagccaggc atcaaataaa acgaaaggct cagtcgaaag actgggcctt
     1981 tcgttttatc tgttgtttgt cggtgaacgc tctctactag agtcacactg gctcaccttc
     2041 gggtgggcct ttctgcgttt atatactaga gtccctatca gtgatagaga ttgacatccc
     2101 tatcagtgat agagatactg agcactacta gatgagcaca aaaaagaaac cattaacaca
     2161 agagcagctt gaggacgcac gtcgccttaa agcaatttat gaaaaaaaga aaaatgaact
     2221 tggcttatcc caggaatctg tcgcagacaa gatggggatg gggcagtcag gcgttggtgc
     2281 tttatttaat ggcatcaatg cattaaatgc ttataacgcc gcattgcttg caaaaattct
     2341 caaagttagc gttgaagaat ttagcccttc aatcgccaga gaaatctacg agatgtatga
     2401 agcggttagt atgcagccgt cacttagaag tgagtatgag taccctgttt tttctcatgt
     2461 tcaggcaggg atgttctcac ctgagcttag aacctttacc aaaggtgatg cggagagatg
     2521 ggtaagcaca accaaaaaag ccagtgattc tgcattctgg cttgaggttg aaggtaattc
     2581 catgaccgca ccaacaggct ccaagccaag ctttcctgac ggaatgttaa ttctcgttga
     2641 ccctgagcag gctgttgagc caggtgattt ctgcatagcc agacttgggg gtgatgagtt
     2701 taccttcaag aaactgatca gggatagcgg tcaggtgttt ttacaaccac taaacccaca
     2761 gtacccaatg atcccatgca atgagagttg ttccgttgtg gggaaagtta tcgctagtca
     2821 gtggcctgaa gagacgtttg gcgctgcaaa cgacgaaaac tacgctttag tagcttaata
     2881 acgctgatag tgctagtgta gatcgctact agagccaggc atcaaataaa acgaaaggct
     2941 cagtcgaaag actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctctactag
     3001 agtcacactg gctcaccttc gggtgggcct ttctgcgttt ata
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