LOCUS       BBa_J01105              1712 bp    DNA     linear       20-Nov-2024
DEFINITION  TEST [OriTF][pLam cI -][str RBS][TraJR][str RBS][GFP][Dbl Term]
ACCESSION   BBa_J01105
VERSION     BBa_J01105.1  
FEATURES             Location/Qualifiers
     oriT            1..365
                     /label=BBa_J01002
     promoter        411..416
                     /label=-10
     promoter        388..393
                     /label=-35
     misc_feature    398..414
                     /label=OR1
     misc_feature    374..390
                     /label=OR2
     misc_feature    374..422
                     /label=BBa_R0051
     promoter        374..422
                     /label=BBa_R0051
     misc_feature    435..438
                     /label=conserved
     RBS             431..442
                     /label=BBa_B0034
     CDS             449..826
                     /label=TraJr
     CDS             449..826
                     /label=BBa_J01001
     misc_feature    431..826
                     /label=BBa_J01021
     misc_feature    374..826
                     /label=BBa_J01103
     misc_feature    1..826
                     /label=BBa_J01104
     RBS             835..849
                     /label=RBS-1\Strong
     RBS             842..846
                     /label=RBS
     misc_feature    835..849
                     /label=BBa_B0030
     RBS             835..849
                     /label=BBa_B0030
     CDS             856..1575
                     /label=GFP protein
     CDS             856..1575
                     /label=BBa_E0040
     stem_loop       1595..1638
                     /label=stem_loop
     misc_feature    1584..1663
                     /label=BBa_B0010
     terminator      1584..1663
                     /label=BBa_B0010
     stem_loop       1679..1698
                     /label=T7 TE
     misc_feature    1705..1705
                     /label=stop
     polyA_site      1699..1712
                     /label=polya
     misc_feature    1672..1712
                     /label=BBa_B0012
     terminator      1672..1712
                     /label=BBa_B0012
     misc_feature    835..1712
                     /label=BBa_E0840
ORIGIN
        1 aaggctcaac aggttggtgg ttctcaccac caaaagcacc acaccccacg caaaaacaag
       61 tttttgctga tttttcttta taaatagagt gttatgaaaa attagtttct cttactctct
      121 ttatgatatt taaaaaagcg gtgtcggcgc ggctacaaca acgcgccgac accgttttgt
      181 aggggtggta ctgactattt ttataaaaaa cattatttta tattaggggt gctgctagcg
      241 gcgcggtgtg tttttttata ggataccgct aggggcgctg ctagcggtgc gtccctgttt
      301 gcattatgaa ttttagtgtt tcgaaattaa ctttatttta tgttcaaaaa aggtaatctc
      361 taatgtacta gagtaacacc gtgcgtgttg actattttac ctctggcggt gataatggtt
      421 gctactagag aaagaggaga aatactagat ggctgatgaa accaagccaa ccaggaaggg
      481 cagcccacct atcaaggtgt actgccttcc agacgaacga agagcgattg aggaaaaggc
      541 ggcggcggcc ggcatgagcc tgtcggccta cctgctggcc gtcggccagg gctacaaaat
      601 cacgggcgtc gtggactatg agcacgtccg cgagctggcc cgcatcaatg gcgacctggg
      661 ccgcctgggc ggcctgctga aactctggct caccgacgac ccgcgcacgg cgcggttcgg
      721 tgatgccacg atcctcgccc tgctggcgaa gatcgaagag aagcaggacg agcttggcaa
      781 ggtcatgatg ggcgtggtcc gcccgagggc agagccatga taataatact agagattaaa
      841 gaggagaaat actagatgcg taaaggagaa gaacttttca ctggagttgt cccaattctt
      901 gttgaattag atggtgatgt taatgggcac aaattttctg tcagtggaga gggtgaaggt
      961 gatgcaacat acggaaaact tacccttaaa tttatttgca ctactggaaa actacctgtt
     1021 ccatggccaa cacttgtcac tactttcggt tatggtgttc aatgctttgc gagataccca
     1081 gatcatatga aacagcatga ctttttcaag agtgccatgc ccgaaggtta tgtacaggaa
     1141 agaactatat ttttcaaaga tgacgggaac tacaagacac gtgctgaagt caagtttgaa
     1201 ggtgataccc ttgttaatag aatcgagtta aaaggtattg attttaaaga agatggaaac
     1261 attcttggac acaaattgga atacaactat aactcacaca atgtatacat catggcagac
     1321 aaacaaaaga atggaatcaa agttaacttc aaaattagac acaacattga agatggaagc
     1381 gttcaactag cagaccatta tcaacaaaat actccaattg gcgatggccc tgtcctttta
     1441 ccagacaacc attacctgtc cacacaatct gccctttcga aagatcccaa cgaaaagaga
     1501 gaccacatgg tccttcttga gtttgtaaca gctgctggga ttacacatgg catggatgaa
     1561 ctatacaaat aataatacta gagccaggca tcaaataaaa cgaaaggctc agtcgaaaga
     1621 ctgggccttt cgttttatct gttgtttgtc ggtgaacgct ctctactaga gtcacactgg
     1681 ctcaccttcg ggtgggcctt tctgcgttta ta
//