BBa_J01105
type: ComponentDefinition
persistent identity: BBa J01105
display id: BBa_J01105
version: 1
was generated by: iGEM to SBOL conversion
description: TEST [OriTF][pLam cI -][str RBS][TraJR][str RBS][GFP][Dbl Term]
created: 2005-12-29T12:00:00Z
modified: 2015-08-31T04:08:13Z
owned by: james
owned by: myers
mutable description: TEST [OriTF][pLam cI -][str RBS][TraJR][str RBS][GFP][Dbl Term]
discontinued: false
dominant: false
group u list: _13_
m user id: 0
owner id: 395
owning group id: 13
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Golden Bear
type: DnaRegion
role: SO:0000804
role: partType/Intermediate
component: BBa_E0840
component: BBa_J01104
sequence annotation: BBa_J01104
sequence annotation: BBa_E0840
sequence: https://synbiohub.org/public/igem/BBa_J01105_sequence/1

BBa_E0840
type: Component
persistent identity: component2226248
display id: component2226248
version: 1
top level: BBa_J01105
definition: GFP genera
access: public

BBa_J01104
type: Component
persistent identity: component2226237
display id: component2226237
version: 1
top level: BBa_J01105
definition: BBa_J01104
access: public

BBa_J01104
type: SequenceAnnotation
persistent identity: annotation2226237
display id: annotation2226237
version: 1
top level: BBa_J01105
location: https://synbiohub.org/public/igem/BBa_J01105/annotation2226237/range2226237/1
component: BBa_J01104

https://synbiohub.org/public/igem/BBa_J01105/annotation2226237/range2226237/1
type: Range
persistent identity: range2226237
display id: range2226237
version: 1
top level: BBa_J01105
direction: direction/reverse
start: 1
end: 826
orientation: inline

BBa_E0840
type: SequenceAnnotation
persistent identity: annotation2226248
display id: annotation2226248
version: 1
top level: BBa_J01105
location: https://synbiohub.org/public/igem/BBa_J01105/annotation2226248/range2226248/1
component: BBa_E0840

https://synbiohub.org/public/igem/BBa_J01105/annotation2226248/range2226248/1
type: Range
persistent identity: range2226248
display id: range2226248
version: 1
top level: BBa_J01105
direction: direction/reverse
start: 835
end: 1712
orientation: inline

BBa_J01104
type: ComponentDefinition
persistent identity: BBa J01104
display id: BBa_J01104
version: 1
was generated by: iGEM to SBOL conversion
description: TEST [OriTF][pLam][StrRBS][TraJR]
created: 2005-12-29T12:00:00Z
modified: 2015-08-31T04:08:13Z
owned by: james
owned by: myers
mutable description: TEST [OriTF][pLam][StrRBS][TraJR]
discontinued: false
dominant: false
group u list: _13_
m user id: 0
owner id: 395
owning group id: 13
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Golden Bear
type: DnaRegion
role: partType/Intermediate
role: SO:0000804
component: BBa_J01103
component: BBa_J01002
sequence annotation: BBa_J01002
sequence annotation: BBa_J01103
sequence: https://synbiohub.org/public/igem/BBa_J01104_sequence/1

BBa_J01103
type: Component
persistent identity: component2220278
display id: component2220278
version: 1
top level: BBa_J01104
definition: BBa_J01103
access: public

BBa_J01002
type: Component
persistent identity: component2220267
display id: component2220267
version: 1
top level: BBa_J01104
definition: OriTF
access: public

BBa_J01002
type: SequenceAnnotation
persistent identity: annotation2220267
display id: annotation2220267
version: 1
top level: BBa_J01104
location: https://synbiohub.org/public/igem/BBa_J01104/annotation2220267/range2220267/1
component: BBa_J01002

https://synbiohub.org/public/igem/BBa_J01104/annotation2220267/range2220267/1
type: Range
persistent identity: range2220267
display id: range2220267
version: 1
top level: BBa_J01104
direction: direction/reverse
start: 1
end: 365
orientation: inline

BBa_J01103
type: SequenceAnnotation
persistent identity: annotation2220278
display id: annotation2220278
version: 1
top level: BBa_J01104
location: https://synbiohub.org/public/igem/BBa_J01104/annotation2220278/range2220278/1
component: BBa_J01103

https://synbiohub.org/public/igem/BBa_J01104/annotation2220278/range2220278/1
type: Range
persistent identity: range2220278
display id: range2220278
version: 1
top level: BBa_J01104
direction: direction/reverse
start: 374
end: 826
orientation: inline

GFP genera
type: ComponentDefinition
persistent identity: BBa E0840
display id: BBa_E0840
version: 1
was generated by: iGEM to SBOL conversion
description: GFP generator
created: 2004-10-17T11:00:00Z
modified: 2015-08-31T04:07:26Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: B0030.E0040.B0015
discontinued: false
dominant: true
experience: experience/Works
group u list: _11_1_
m user id: 0
owner id: 61
owning group id: 7
sample status: In stock
status: status/Available
bookmark: true
star: true
creator: Jennifer Braff
type: DnaRegion
role: SO:0000804
role: partType/Reporter
component: BBa_B0030
component: BBa_B0010
component: BBa_E0040
component: BBa_B0012
sequence annotation: BBa_B0030
sequence annotation: BBa_E0040
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_E0840_sequence/1

BBa_B0030
type: Component
persistent identity: component1249239
display id: component1249239
version: 1
top level: GFP genera
definition: BBa_B0030
access: public

BBa_B0010
type: Component
persistent identity: component1249247
display id: component1249247
version: 1
top level: GFP genera
definition: BBa_B0010
access: public

BBa_E0040
type: Component
persistent identity: component1249242
display id: component1249242
version: 1
top level: GFP genera
definition: GFP
access: public

BBa_B0012
type: Component
persistent identity: component1249257
display id: component1249257
version: 1
top level: GFP genera
definition: BBa_B0012
access: public

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation1249239
display id: annotation1249239
version: 1
top level: GFP genera
location: https://synbiohub.org/public/igem/BBa_E0840/annotation1249239/range1249239/1
component: BBa_B0030

https://synbiohub.org/public/igem/BBa_E0840/annotation1249239/range1249239/1
type: Range
persistent identity: range1249239
display id: range1249239
version: 1
top level: GFP genera
direction: direction/forward
start: 1
end: 15
orientation: inline

BBa_E0040
type: SequenceAnnotation
persistent identity: annotation1249242
display id: annotation1249242
version: 1
top level: GFP genera
location: https://synbiohub.org/public/igem/BBa_E0840/annotation1249242/range1249242/1
component: BBa_E0040

https://synbiohub.org/public/igem/BBa_E0840/annotation1249242/range1249242/1
type: Range
persistent identity: range1249242
display id: range1249242
version: 1
top level: GFP genera
direction: direction/forward
start: 22
end: 741
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1249247
display id: annotation1249247
version: 1
top level: GFP genera
location: https://synbiohub.org/public/igem/BBa_E0840/annotation1249247/range1249247/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_E0840/annotation1249247/range1249247/1
type: Range
persistent identity: range1249247
display id: range1249247
version: 1
top level: GFP genera
direction: direction/forward
start: 750
end: 829
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1249257
display id: annotation1249257
version: 1
top level: GFP genera
location: https://synbiohub.org/public/igem/BBa_E0840/annotation1249257/range1249257/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_E0840/annotation1249257/range1249257/1
type: Range
persistent identity: range1249257
display id: range1249257
version: 1
top level: GFP genera
direction: direction/forward
start: 838
end: 878
orientation: inline

BBa_J01103
type: ComponentDefinition
persistent identity: BBa J01103
display id: BBa_J01103
version: 1
was generated by: iGEM to SBOL conversion
description: TEST [pcI Lam][StrRBS][TraJR]
created: 2005-12-29T12:00:00Z
modified: 2015-08-31T04:08:13Z
owned by: james
owned by: myers
mutable description: TEST [pLam][StrRBS][TraJR]
discontinued: false
dominant: false
group u list: _13_
m user id: 0
owner id: 395
owning group id: 13
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Golden Bear
type: DnaRegion
role: partType/Intermediate
role: SO:0000804
component: BBa_J01021
component: BBa_R0051
sequence annotation: BBa_R0051
sequence annotation: BBa_J01021
sequence: https://synbiohub.org/public/igem/BBa_J01103_sequence/1

BBa_J01021
type: Component
persistent identity: component2219293
display id: component2219293
version: 1
top level: BBa_J01103
definition: StrRBS.Tra
access: public

BBa_R0051
type: Component
persistent identity: component2219285
display id: component2219285
version: 1
top level: BBa_J01103
definition: cI lam
access: public

BBa_R0051
type: SequenceAnnotation
persistent identity: annotation2219285
display id: annotation2219285
version: 1
top level: BBa_J01103
location: https://synbiohub.org/public/igem/BBa_J01103/annotation2219285/range2219285/1
component: BBa_R0051

https://synbiohub.org/public/igem/BBa_J01103/annotation2219285/range2219285/1
type: Range
persistent identity: range2219285
display id: range2219285
version: 1
top level: BBa_J01103
direction: direction/forward
start: 1
end: 49
orientation: inline

BBa_J01021
type: SequenceAnnotation
persistent identity: annotation2219293
display id: annotation2219293
version: 1
top level: BBa_J01103
location: https://synbiohub.org/public/igem/BBa_J01103/annotation2219293/range2219293/1
component: BBa_J01021

https://synbiohub.org/public/igem/BBa_J01103/annotation2219293/range2219293/1
type: Range
persistent identity: range2219293
display id: range2219293
version: 1
top level: BBa_J01103
direction: direction/reverse
start: 58
end: 453
orientation: inline

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: SO:0000313
role: feature/stem loop

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: feature/polya
role: SO:0000553

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

BBa_B0030
type: ComponentDefinition
persistent identity: BBa B0030
display id: BBa_B0030
version: 1
was generated by: iGEM to SBOL conversion
description: RBS.1 (strong) -- modified from R. Weiss
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Strong RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0032</bb_part>, <bb_part>BBa_B0033</bb_part>.
discontinued: false
dominant: true
experience: experience/Works
group u list: _44_46_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("orig" in figure 4-14 of Ron Weiss thesis). No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: RBS-1\Strong
sequence annotation: BBa_B0030
sequence annotation: RBS
sequence: https://synbiohub.org/public/igem/BBa_B0030_sequence/1

RBS-1\Strong
type: SequenceAnnotation
persistent identity: annotation1701
display id: annotation1701
version: 1
top level: BBa_B0030
location: https://synbiohub.org/public/igem/BBa_B0030/annotation1701/range1701/1
role: SO:0000139
role: feature/rbs

https://synbiohub.org/public/igem/BBa_B0030/annotation1701/range1701/1
type: Range
persistent identity: range1701
display id: range1701
version: 1
top level: BBa_B0030
direction: direction/forward
start: 1
end: 15
orientation: inline

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation7025
display id: annotation7025
version: 1
top level: BBa_B0030
location: https://synbiohub.org/public/igem/BBa_B0030/annotation7025/range7025/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0030/annotation7025/range7025/1
type: Range
persistent identity: range7025
display id: range7025
version: 1
top level: BBa_B0030
direction: direction/forward
start: 1
end: 15
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation1702
display id: annotation1702
version: 1
top level: BBa_B0030
location: https://synbiohub.org/public/igem/BBa_B0030/annotation1702/range1702/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_B0030/annotation1702/range1702/1
type: Range
persistent identity: range1702
display id: range1702
version: 1
top level: BBa_B0030
direction: direction/forward
start: 8
end: 12
orientation: inline

GFP
type: ComponentDefinition
persistent identity: BBa E0040
display id: BBa_E0040
version: 1
was generated by: iGEM to SBOL conversion
description: green fluorescent protein derived from jellyfish Aequeora victoria wild-type GFP (SwissProt: P42212
created: 2004-09-29T11:00:00Z
modified: 2016-01-26T02:09:38Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: GFP (mut3b) [note that this part does not have a barcode]
discontinued: false
dominant: true
experience: experience/Works
group u list: _11_1_
m user id: 4206
owner id: 61
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: jcbraff
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: GFP protein
sequence: https://synbiohub.org/public/igem/BBa_E0040_sequence/1

GFP protein
type: SequenceAnnotation
persistent identity: annotation1934520
display id: annotation1934520
version: 1
top level: GFP
location: https://synbiohub.org/public/igem/BBa_E0040/annotation1934520/range1934520/1
role: SO:0000316
role: feature/protein

https://synbiohub.org/public/igem/BBa_E0040/annotation1934520/range1934520/1
type: Range
persistent identity: range1934520
display id: range1934520
version: 1
top level: GFP
direction: direction/forward
start: 1
end: 720
orientation: inline

OriTF
type: ComponentDefinition
persistent identity: BBa J01002
display id: BBa_J01002
version: 1
was generated by: iGEM to SBOL conversion
description: OriT-F (Origin of Transfer for the F-plasmid nic region)
created: 2005-10-17T11:00:00Z
modified: 2015-08-31T04:08:11Z
owned by: james
owned by: myers
mutable description: OriTf (Origin of Transfer for the F-type conjugative plasmid) is the nic region, is where the relaxosome nicks the plasmid and conjugative transfer by F plasmid machinery begins via rolling circle replication. This particular plasmid has been derived from a F-type plasmid donated by Professor Laura Frost University of Alberta. NOTE: OriTf is NOT the same as the host cell's native Origin of Transfer (this is a separate site within the bacterial host's main genome).
discontinued: false
dominant: false
experience: experience/Works
group u list: _13_
m user id: 0
owner id: 395
owning group id: 13
sample status: It's complicated
status: status/Planning
bookmark: false
star: true
creator: Golden Bear
type: DnaRegion
role: SO:0000724
role: partType/Conjugation
sequence: https://synbiohub.org/public/igem/BBa_J01002_sequence/1

StrRBS.Tra
type: ComponentDefinition
persistent identity: BBa J01021
display id: BBa_J01021
version: 1
was generated by: iGEM to SBOL conversion
description: [StrRBS][TraJR]
created: 2005-11-05T12:00:00Z
modified: 2015-08-31T04:08:12Z
owned by: james
owned by: myers
mutable description: [StrRBS][TraJR]
discontinued: false
dominant: false
group u list: _13_
m user id: 0
owner id: 395
owning group id: 13
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Golden Bear
type: DnaRegion
role: partType/Intermediate
role: SO:0000804
component: BBa_B0034
component: BBa_J01001
sequence annotation: BBa_B0034
sequence annotation: BBa_J01001
sequence: https://synbiohub.org/public/igem/BBa_J01021_sequence/1

BBa_B0034
type: Component
persistent identity: component2218193
display id: component2218193
version: 1
top level: StrRBS.Tra
definition: BBa_B0034
access: public

BBa_J01001
type: Component
persistent identity: component2218195
display id: component2218195
version: 1
top level: StrRBS.Tra
definition: TraJR
access: public

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2218193
display id: annotation2218193
version: 1
top level: StrRBS.Tra
location: https://synbiohub.org/public/igem/BBa_J01021/annotation2218193/range2218193/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_J01021/annotation2218193/range2218193/1
type: Range
persistent identity: range2218193
display id: range2218193
version: 1
top level: StrRBS.Tra
direction: direction/reverse
start: 1
end: 12
orientation: inline

BBa_J01001
type: SequenceAnnotation
persistent identity: annotation2218195
display id: annotation2218195
version: 1
top level: StrRBS.Tra
location: https://synbiohub.org/public/igem/BBa_J01021/annotation2218195/range2218195/1
component: BBa_J01001

https://synbiohub.org/public/igem/BBa_J01021/annotation2218195/range2218195/1
type: Range
persistent identity: range2218195
display id: range2218195
version: 1
top level: StrRBS.Tra
direction: direction/reverse
start: 19
end: 396
orientation: inline

cI lam
type: ComponentDefinition
persistent identity: BBa R0051
display id: BBa_R0051
version: 1
was generated by: iGEM to SBOL conversion
description: promoter (lambda cI regulated)
created: 2003-01-31T12:00:00Z
modified: 2015-05-08T01:14:14Z
mutable provenance: A synthetic oscillatory network of transcriptional regulators , Elowitz M.B. , Leibler S., Nature(403),335-38: 2000
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: The cI regulated promoter is based on the pR promtoer from bacteriohage lambda. The promoter has two two DNA binding sites for lambda cI repressor <bb_part>BBa_C0051</bb_part>. cI binding results in repression of transcription. The specific sequence used here is based on the cI repressible promoter used in the Elowitz repressilator (and references therein).
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: In order to address concerns about the promoter transcribing in the reverse direction, we have removed the -35 and -10 signals responsible for the promoter activity in the reverse direction. (More details needed here! DE, 2/24/03) Incompatible with host expressing cI repressor.
star: true
creator: Vinay S Mahajan, Brian Chow, Peter Carr, Voichita Marinescu and Alexander D. Wissner-Gross
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
sequence annotation: BBa_R0051
sequence annotation: OR2
sequence annotation: -35
sequence annotation: OR1
sequence annotation: -10
sequence: https://synbiohub.org/public/igem/BBa_R0051_sequence/1

BBa_R0051
type: SequenceAnnotation
persistent identity: annotation7067
display id: annotation7067
version: 1
top level: cI lam
location: https://synbiohub.org/public/igem/BBa_R0051/annotation7067/range7067/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_R0051/annotation7067/range7067/1
type: Range
persistent identity: range7067
display id: range7067
version: 1
top level: cI lam
direction: direction/forward
start: 1
end: 49
orientation: inline

OR2
type: SequenceAnnotation
persistent identity: annotation2025
display id: annotation2025
version: 1
top level: cI lam
location: https://synbiohub.org/public/igem/BBa_R0051/annotation2025/range2025/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_R0051/annotation2025/range2025/1
type: Range
persistent identity: range2025
display id: range2025
version: 1
top level: cI lam
direction: direction/forward
start: 1
end: 17
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation2023
display id: annotation2023
version: 1
top level: cI lam
location: https://synbiohub.org/public/igem/BBa_R0051/annotation2023/range2023/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_R0051/annotation2023/range2023/1
type: Range
persistent identity: range2023
display id: range2023
version: 1
top level: cI lam
direction: direction/forward
start: 15
end: 20
orientation: inline

OR1
type: SequenceAnnotation
persistent identity: annotation2024
display id: annotation2024
version: 1
top level: cI lam
location: https://synbiohub.org/public/igem/BBa_R0051/annotation2024/range2024/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_R0051/annotation2024/range2024/1
type: Range
persistent identity: range2024
display id: range2024
version: 1
top level: cI lam
direction: direction/forward
start: 25
end: 41
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation2022
display id: annotation2022
version: 1
top level: cI lam
location: https://synbiohub.org/public/igem/BBa_R0051/annotation2022/range2022/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_R0051/annotation2022/range2022/1
type: Range
persistent identity: range2022
display id: range2022
version: 1
top level: cI lam
direction: direction/forward
start: 38
end: 43
orientation: inline

TraJR
type: ComponentDefinition
persistent identity: BBa J01001
display id: BBa_J01001
version: 1
was generated by: iGEM to SBOL conversion
description: TraJr protein (controls conjugative transfer in RP4 plasmid)
created: 2005-10-17T11:00:00Z
modified: 2015-08-31T04:08:11Z
owned by: james
owned by: myers
mutable description: TraJR controls conjugative transfer in R plasmid
discontinued: false
dominant: false
experience: experience/None
group u list: _13_
m user id: 0
owner id: 395
owning group id: 13
sample status: It's complicated
status: status/Planning
bookmark: false
star: true
creator: Berkeley iGEM 2005
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: TraJr
sequence: https://synbiohub.org/public/igem/BBa_J01001_sequence/1

TraJr
type: SequenceAnnotation
persistent identity: annotation1891611
display id: annotation1891611
version: 1
top level: TraJR
location: https://synbiohub.org/public/igem/BBa_J01001/annotation1891611/range1891611/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_J01001/annotation1891611/range1891611/1
type: Range
persistent identity: range1891611
display id: range1891611
version: 1
top level: TraJR
direction: direction/forward
start: 1
end: 378
orientation: inline

BBa_B0034
type: ComponentDefinition
persistent identity: BBa B0034
display id: BBa_B0034
version: 1
was generated by: iGEM to SBOL conversion
description: RBS (Elowitz 1999) -- defines RBS efficiency
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: RBS based on Elowitz repressilator.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: conserved
sequence: https://synbiohub.org/public/igem/BBa_B0034_sequence/1

conserved
type: SequenceAnnotation
persistent identity: annotation23325
display id: annotation23325
version: 1
top level: BBa_B0034
location: https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
role: SO:0000330
role: feature/conserved

https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
type: Range
persistent identity: range23325
display id: range23325
version: 1
top level: BBa_B0034
direction: direction/forward
start: 5
end: 8
orientation: inline

https://synbiohub.org/public/igem/BBa_J01105_sequence/1
type: Sequence
persistent identity: BBa J01105 sequence
display id: BBa_J01105_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaggctcaacaggttggtggttctcaccaccaaaagcaccacaccccacgcaaaaacaagtttttgctgatttttctttataaatagagtgttatgaaaaattagtttctcttactctctttatgatatttaaaaaagcggtgtcggcgcggctacaacaacgcgccgacaccgttttgtaggggtggtactgactatttttataaaaaacattattttatattaggggtgctgctagcggcgcggtgtgtttttttataggataccgctaggggcgctgctagcggtgcgtccctgtttgcattatgaattttagtgtttcgaaattaactttattttatgttcaaaaaaggtaatctctaatgtactagagtaacaccgtgcgtgttgactattttacctctggcggtgataatggttgctactagagaaagaggagaaatactagatggctgatgaaaccaagccaaccaggaagggcagcccacctatcaaggtgtactgccttccagacgaacgaagagcgattgaggaaaaggcggcggcggccggcatgagcctgtcggcctacctgctggccgtcggccagggctacaaaatcacgggcgtcgtggactatgagcacgtccgcgagctggcccgcatcaatggcgacctgggccgcctgggcggcctgctgaaactctggctcaccgacgacccgcgcacggcgcggttcggtgatgccacgatcctcgccctgctggcgaagatcgaagagaagcaggacgagcttggcaaggtcatgatgggcgtggtccgcccgagggcagagccatgataataatactagagattaaagaggagaaatactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J01104_sequence/1
type: Sequence
persistent identity: BBa J01104 sequence
display id: BBa_J01104_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaggctcaacaggttggtggttctcaccaccaaaagcaccacaccccacgcaaaaacaagtttttgctgatttttctttataaatagagtgttatgaaaaattagtttctcttactctctttatgatatttaaaaaagcggtgtcggcgcggctacaacaacgcgccgacaccgttttgtaggggtggtactgactatttttataaaaaacattattttatattaggggtgctgctagcggcgcggtgtgtttttttataggataccgctaggggcgctgctagcggtgcgtccctgtttgcattatgaattttagtgtttcgaaattaactttattttatgttcaaaaaaggtaatctctaatgtactagagtaacaccgtgcgtgttgactattttacctctggcggtgataatggttgctactagagaaagaggagaaatactagatggctgatgaaaccaagccaaccaggaagggcagcccacctatcaaggtgtactgccttccagacgaacgaagagcgattgaggaaaaggcggcggcggccggcatgagcctgtcggcctacctgctggccgtcggccagggctacaaaatcacgggcgtcgtggactatgagcacgtccgcgagctggcccgcatcaatggcgacctgggccgcctgggcggcctgctgaaactctggctcaccgacgacccgcgcacggcgcggttcggtgatgccacgatcctcgccctgctggcgaagatcgaagagaagcaggacgagcttggcaaggtcatgatgggcgtggtccgcccgagggcagagccatgataataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_E0840_sequence/1
type: Sequence
persistent identity: BBa E0840 sequence
display id: BBa_E0840_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: attaaagaggagaaatactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J01103_sequence/1
type: Sequence
persistent identity: BBa J01103 sequence
display id: BBa_J01103_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: taacaccgtgcgtgttgactattttacctctggcggtgataatggttgctactagagaaagaggagaaatactagatggctgatgaaaccaagccaaccaggaagggcagcccacctatcaaggtgtactgccttccagacgaacgaagagcgattgaggaaaaggcggcggcggccggcatgagcctgtcggcctacctgctggccgtcggccagggctacaaaatcacgggcgtcgtggactatgagcacgtccgcgagctggcccgcatcaatggcgacctgggccgcctgggcggcctgctgaaactctggctcaccgacgacccgcgcacggcgcggttcggtgatgccacgatcctcgccctgctggcgaagatcgaagagaagcaggacgagcttggcaaggtcatgatgggcgtggtccgcccgagggcagagccatgataataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0030_sequence/1
type: Sequence
persistent identity: BBa B0030 sequence
display id: BBa_B0030_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: attaaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_E0040_sequence/1
type: Sequence
persistent identity: BBa E0040 sequence
display id: BBa_E0040_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: myers
owned by: james
elements: atgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J01002_sequence/1
type: Sequence
persistent identity: BBa J01002 sequence
display id: BBa_J01002_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaggctcaacaggttggtggttctcaccaccaaaagcaccacaccccacgcaaaaacaagtttttgctgatttttctttataaatagagtgttatgaaaaattagtttctcttactctctttatgatatttaaaaaagcggtgtcggcgcggctacaacaacgcgccgacaccgttttgtaggggtggtactgactatttttataaaaaacattattttatattaggggtgctgctagcggcgcggtgtgtttttttataggataccgctaggggcgctgctagcggtgcgtccctgtttgcattatgaattttagtgtttcgaaattaactttattttatgttcaaaaaaggtaatctctaatg
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J01021_sequence/1
type: Sequence
persistent identity: BBa J01021 sequence
display id: BBa_J01021_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaatactagatggctgatgaaaccaagccaaccaggaagggcagcccacctatcaaggtgtactgccttccagacgaacgaagagcgattgaggaaaaggcggcggcggccggcatgagcctgtcggcctacctgctggccgtcggccagggctacaaaatcacgggcgtcgtggactatgagcacgtccgcgagctggcccgcatcaatggcgacctgggccgcctgggcggcctgctgaaactctggctcaccgacgacccgcgcacggcgcggttcggtgatgccacgatcctcgccctgctggcgaagatcgaagagaagcaggacgagcttggcaaggtcatgatgggcgtggtccgcccgagggcagagccatgataataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_R0051_sequence/1
type: Sequence
persistent identity: BBa R0051 sequence
display id: BBa_R0051_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: taacaccgtgcgtgttgactattttacctctggcggtgataatggttgc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J01001_sequence/1
type: Sequence
persistent identity: BBa J01001 sequence
display id: BBa_J01001_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggctgatgaaaccaagccaaccaggaagggcagcccacctatcaaggtgtactgccttccagacgaacgaagagcgattgaggaaaaggcggcggcggccggcatgagcctgtcggcctacctgctggccgtcggccagggctacaaaatcacgggcgtcgtggactatgagcacgtccgcgagctggcccgcatcaatggcgacctgggccgcctgggcggcctgctgaaactctggctcaccgacgacccgcgcacggcgcggttcggtgatgccacgatcctcgccctgctggcgaagatcgaagagaagcaggacgagcttggcaaggtcatgatgggcgtggtccgcccgagggcagagccatgataataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0034_sequence/1
type: Sequence
persistent identity: BBa B0034 sequence
display id: BBa_B0034_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaa
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: Chris J. Myers
creator: James Alastair McLaughlin
ended at time: 2017-03-06T15:00:00.000Z