LOCUS       BBa_J01136              2436 bp    DNA     linear       20-Nov-2024
DEFINITION  TEST [OriTF][pBad/araC][StrRBS][TraJF][DblTerm]
ACCESSION   BBa_J01136
VERSION     BBa_J01136.1  
FEATURES             Location/Qualifiers
     oriT            1..365
                     /label=BBa_J01002
     promoter        1453..1583
                     /label=Pbad
     promoter        1416..1487
                     /label=PC
     misc_feature    374..1452
                     /label=AraC
     promoter        374..1583
                     /label=BBa_I0500
     misc_feature    1596..1599
                     /label=conserved
     RBS             1592..1603
                     /label=BBa_B0034
     CDS             1610..2299
                     /label=BBa_J01000
     stem_loop       2319..2362
                     /label=stem_loop
     misc_feature    2308..2387
                     /label=BBa_B0010
     terminator      2308..2387
                     /label=BBa_B0010
     stem_loop       2403..2422
                     /label=T7 TE
     misc_feature    2429..2429
                     /label=stop
     polyA_site      2423..2436
                     /label=polya
     misc_feature    2396..2436
                     /label=BBa_B0012
     terminator      2396..2436
                     /label=BBa_B0012
ORIGIN
        1 aaggctcaac aggttggtgg ttctcaccac caaaagcacc acaccccacg caaaaacaag
       61 tttttgctga tttttcttta taaatagagt gttatgaaaa attagtttct cttactctct
      121 ttatgatatt taaaaaagcg gtgtcggcgc ggctacaaca acgcgccgac accgttttgt
      181 aggggtggta ctgactattt ttataaaaaa cattatttta tattaggggt gctgctagcg
      241 gcgcggtgtg tttttttata ggataccgct aggggcgctg ctagcggtgc gtccctgttt
      301 gcattatgaa ttttagtgtt tcgaaattaa ctttatttta tgttcaaaaa aggtaatctc
      361 taatgtacta gagttatgac aacttgacgg ctacatcatt cactttttct tcacaaccgg
      421 cacggaactc gctcgggctg gccccggtgc attttttaaa tacccgcgag aaatagagtt
      481 gatcgtcaaa accaacattg cgaccgacgg tggcgatagg catccgggtg gtgctcaaaa
      541 gcagcttcgc ctggctgata cgttggtcct cgcgccagct taagacgcta atccctaact
      601 gctggcggaa aagatgtgac agacgcgacg gcgacaagca aacatgctgt gcgacgctgg
      661 cgatatcaaa attgctgtct gccaggtgat cgctgatgta ctgacaagcc tcgcgtaccc
      721 gattatccat cggtggatgg agcgactcgt taatcgcttc catgcgccgc agtaacaatt
      781 gctcaagcag atttatcgcc agcagctccg aatagcgccc ttccccttgc ccggcgttaa
      841 tgatttgccc aaacaggtcg ctgaaatgcg gctggtgcgc ttcatccggg cgaaagaacc
      901 ccgtattggc aaatattgac ggccagttaa gccattcatg ccagtaggcg cgcggacgaa
      961 agtaaaccca ctggtgatac cattcgcgag cctccggatg acgaccgtag tgatgaatct
     1021 ctcctggcgg gaacagcaaa atatcacccg gtcggcaaac aaattctcgt ccctgatttt
     1081 tcaccacccc ctgaccgcga atggtgagat tgagaatata acctttcatt cccagcggtc
     1141 ggtcgataaa aaaatcgaga taaccgttgg cctcaatcgg cgttaaaccc gccaccagat
     1201 gggcattaaa cgagtatccc ggcagcaggg gatcattttg cgcttcagcc atacttttca
     1261 tactcccgcc attcagagaa gaaaccaatt gtccatattg catcagacat tgccgtcact
     1321 gcgtctttta ctggctcttc tcgctaacca aaccggtaac cccgcttatt aaaagcattc
     1381 tgtaacaaag cgggaccaaa gccatgacaa aaacgcgtaa caaaagtgtc tataatcacg
     1441 gcagaaaagt ccacattgat tatttgcacg gcgtcacact ttgctatgcc atagcatttt
     1501 tatccataag attagcggat cctacctgac gctttttatc gcaactctct actgtttctc
     1561 catacccgtt tttttgggct agctactaga gaaagaggag aaatactaga tgtatccgat
     1621 ggatcgtatt caacaaaaac atgctcgtca aatagatctg ctggaaaatc tgacggcagt
     1681 tattcaggat tatccaaatc cagcctgtat cagggacgaa actggaaaat ttattttttg
     1741 caatacgctg tttcatgagt catttcttac acaagatcaa agtgctgaaa aatggcttct
     1801 gtcgcagaga gatttttgtg aattgatctc tgtcacagag atggaagcat ataggaatga
     1861 gcatacgcat cttaatcttg tagaggatgt ttttattcag aatagattct ggacaatatc
     1921 tgtccagtca tttcttaatg gacacagaaa tattattctg tggcaatttt atgatgctgc
     1981 tcatgttcgt cataaagaca gttataatca aaaaacgatt gtcagtgatg atatcagaaa
     2041 tataatcaga agaatgagtg atgattcttc tgtatcatca tatgtaaatg atgtctttta
     2101 cttatatagc accggaatca gtcataatgc tatagcaaga atattaaata tatccatctc
     2161 cacatcaaag aaacacgcat ctctgatatg cgactacttc tctgtttcta ataaagatga
     2221 gttaattatc ttactctaca ataaaaagtt tatttattat ttatacgaga aggctatgtg
     2281 tatcataaat acgcgttaat actagagcca ggcatcaaat aaaacgaaag gctcagtcga
     2341 aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa cgctctctac tagagtcaca
     2401 ctggctcacc ttcgggtggg cctttctgcg tttata
//