BBa_I0500 1 pBad/araC Inducible pBad/araC promoter 2003-12-05T12:00:00Z 2015-08-31T04:07:29Z -- No description -- false true _1_ 0 24 7 In stock false true Sri Kosuri annotation1498842 1 PC range1498842 1 1043 1114 annotation1498841 1 Pbad range1498841 1 1080 1210 annotation1498843 1 AraC range1498843 1 1 1079 BBa_B0012 1 BBa_B0012 TE from coliphageT7 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>. Released HQ 2013 Transcription terminator for the <i>E.coli</i> RNA polymerase. false false _1_ 0 24 7 In stock false <P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator. false Reshma Shetty annotation1686 1 T7 TE range1686 1 8 27 annotation1687 1 stop range1687 1 34 34 annotation1690 1 polya range1690 1 28 41 annotation7020 1 BBa_B0012 range7020 1 1 41 BBa_B0010 1 BBa_B0010 T1 from E. coli rrnB 2003-11-19T12:00:00Z 2015-08-31T04:07:20Z Transcriptional terminator consisting of a 64 bp stem-loop. false false _1_ 0 24 7 In stock false true Randy Rettberg annotation4184 1 stem_loop range4184 1 12 55 annotation7018 1 BBa_B0010 range7018 1 1 80 BBa_J01136 1 BBa_J01136 TEST [OriTF][pBad/araC][StrRBS][TraJF][DblTerm] 2006-01-19T12:00:00Z 2015-08-31T04:08:13Z TEST [OriTF][pBad/araC][StrRBS][TraJF][DblTerm] false false _13_ 0 395 13 Not in stock false false Golden Bear component1789568 1 BBa_B0010 component1789545 1 BBa_J01002 component1789556 1 BBa_I0500 component1789578 1 BBa_B0012 component1789563 1 BBa_J01000 component1789561 1 BBa_B0034 annotation1789578 1 BBa_B0012 range1789578 1 2396 2436 annotation1789545 1 BBa_J01002 range1789545 1 1 365 annotation1789556 1 BBa_I0500 range1789556 1 374 1583 annotation1789561 1 BBa_B0034 range1789561 1 1592 1603 annotation1789568 1 BBa_B0010 range1789568 1 2308 2387 annotation1789563 1 BBa_J01000 range1789563 1 1610 2299 BBa_B0034 1 BBa_B0034 RBS (Elowitz 1999) -- defines RBS efficiency 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 RBS based on Elowitz repressilator. false true _1_ 0 24 7 In stock false Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation23325 1 conserved range23325 1 5 8 BBa_J01002 1 OriTF OriT-F (Origin of Transfer for the F-plasmid nic region) 2005-10-17T11:00:00Z 2015-08-31T04:08:11Z OriTf (Origin of Transfer for the F-type conjugative plasmid) is the nic region, is where the relaxosome nicks the plasmid and conjugative transfer by F plasmid machinery begins via rolling circle replication. <br> This particular plasmid has been derived from a F-type plasmid donated by Professor Laura Frost University of Alberta. <br> <small> NOTE: OriTf is NOT the same as the host cell's native Origin of Transfer (this is a separate site within the bacterial host's main genome). <br> </small> false false _13_ 0 395 13 It's complicated false true Golden Bear BBa_J01000 1 TraJF TraJF controls conjugative transfer in F plasmid 2005-10-17T11:00:00Z 2015-08-31T04:08:11Z TraJF controls conjugative transfer in F plasmid false false _13_ 0 395 13 Not in stock false false Berkeley iGEM 2005 BBa_B0010_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc BBa_J01000_sequence 1 atgtatccgatggatcgtattcaacaaaaacatgctcgtcaaatagatctgctggaaaatctgacggcagttattcaggattatccaaatccagcctgtatcagggacgaaactggaaaatttattttttgcaatacgctgtttcatgagtcatttcttacacaagatcaaagtgctgaaaaatggcttctgtcgcagagagatttttgtgaattgatctctgtcacagagatggaagcatataggaatgagcatacgcatcttaatcttgtagaggatgtttttattcagaatagattctggacaatatctgtccagtcatttcttaatggacacagaaatattattctgtggcaattttatgatgctgctcatgttcgtcataaagacagttataatcaaaaaacgattgtcagtgatgatatcagaaatataatcagaagaatgagtgatgattcttctgtatcatcatatgtaaatgatgtcttttacttatatagcaccggaatcagtcataatgctatagcaagaatattaaatatatccatctccacatcaaagaaacacgcatctctgatatgcgactacttctctgtttctaataaagatgagttaattatcttactctacaataaaaagtttatttattatttatacgagaaggctatgtgtatcataaatacgcgttaa BBa_B0034_sequence 1 aaagaggagaaa BBa_J01002_sequence 1 aaggctcaacaggttggtggttctcaccaccaaaagcaccacaccccacgcaaaaacaagtttttgctgatttttctttataaatagagtgttatgaaaaattagtttctcttactctctttatgatatttaaaaaagcggtgtcggcgcggctacaacaacgcgccgacaccgttttgtaggggtggtactgactatttttataaaaaacattattttatattaggggtgctgctagcggcgcggtgtgtttttttataggataccgctaggggcgctgctagcggtgcgtccctgtttgcattatgaattttagtgtttcgaaattaactttattttatgttcaaaaaaggtaatctctaatg BBa_I0500_sequence 1 ttatgacaacttgacggctacatcattcactttttcttcacaaccggcacggaactcgctcgggctggccccggtgcattttttaaatacccgcgagaaatagagttgatcgtcaaaaccaacattgcgaccgacggtggcgataggcatccgggtggtgctcaaaagcagcttcgcctggctgatacgttggtcctcgcgccagcttaagacgctaatccctaactgctggcggaaaagatgtgacagacgcgacggcgacaagcaaacatgctgtgcgacgctggcgatatcaaaattgctgtctgccaggtgatcgctgatgtactgacaagcctcgcgtacccgattatccatcggtggatggagcgactcgttaatcgcttccatgcgccgcagtaacaattgctcaagcagatttatcgccagcagctccgaatagcgcccttccccttgcccggcgttaatgatttgcccaaacaggtcgctgaaatgcggctggtgcgcttcatccgggcgaaagaaccccgtattggcaaatattgacggccagttaagccattcatgccagtaggcgcgcggacgaaagtaaacccactggtgataccattcgcgagcctccggatgacgaccgtagtgatgaatctctcctggcgggaacagcaaaatatcacccggtcggcaaacaaattctcgtccctgatttttcaccaccccctgaccgcgaatggtgagattgagaatataacctttcattcccagcggtcggtcgataaaaaaatcgagataaccgttggcctcaatcggcgttaaacccgccaccagatgggcattaaacgagtatcccggcagcaggggatcattttgcgcttcagccatacttttcatactcccgccattcagagaagaaaccaattgtccatattgcatcagacattgccgtcactgcgtcttttactggctcttctcgctaaccaaaccggtaaccccgcttattaaaagcattctgtaacaaagcgggaccaaagccatgacaaaaacgcgtaacaaaagtgtctataatcacggcagaaaagtccacattgattatttgcacggcgtcacactttgctatgccatagcatttttatccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccatacccgtttttttgggctagc BBa_B0012_sequence 1 tcacactggctcaccttcgggtgggcctttctgcgtttata BBa_J01136_sequence 1 aaggctcaacaggttggtggttctcaccaccaaaagcaccacaccccacgcaaaaacaagtttttgctgatttttctttataaatagagtgttatgaaaaattagtttctcttactctctttatgatatttaaaaaagcggtgtcggcgcggctacaacaacgcgccgacaccgttttgtaggggtggtactgactatttttataaaaaacattattttatattaggggtgctgctagcggcgcggtgtgtttttttataggataccgctaggggcgctgctagcggtgcgtccctgtttgcattatgaattttagtgtttcgaaattaactttattttatgttcaaaaaaggtaatctctaatgtactagagttatgacaacttgacggctacatcattcactttttcttcacaaccggcacggaactcgctcgggctggccccggtgcattttttaaatacccgcgagaaatagagttgatcgtcaaaaccaacattgcgaccgacggtggcgataggcatccgggtggtgctcaaaagcagcttcgcctggctgatacgttggtcctcgcgccagcttaagacgctaatccctaactgctggcggaaaagatgtgacagacgcgacggcgacaagcaaacatgctgtgcgacgctggcgatatcaaaattgctgtctgccaggtgatcgctgatgtactgacaagcctcgcgtacccgattatccatcggtggatggagcgactcgttaatcgcttccatgcgccgcagtaacaattgctcaagcagatttatcgccagcagctccgaatagcgcccttccccttgcccggcgttaatgatttgcccaaacaggtcgctgaaatgcggctggtgcgcttcatccgggcgaaagaaccccgtattggcaaatattgacggccagttaagccattcatgccagtaggcgcgcggacgaaagtaaacccactggtgataccattcgcgagcctccggatgacgaccgtagtgatgaatctctcctggcgggaacagcaaaatatcacccggtcggcaaacaaattctcgtccctgatttttcaccaccccctgaccgcgaatggtgagattgagaatataacctttcattcccagcggtcggtcgataaaaaaatcgagataaccgttggcctcaatcggcgttaaacccgccaccagatgggcattaaacgagtatcccggcagcaggggatcattttgcgcttcagccatacttttcatactcccgccattcagagaagaaaccaattgtccatattgcatcagacattgccgtcactgcgtcttttactggctcttctcgctaaccaaaccggtaaccccgcttattaaaagcattctgtaacaaagcgggaccaaagccatgacaaaaacgcgtaacaaaagtgtctataatcacggcagaaaagtccacattgattatttgcacggcgtcacactttgctatgccatagcatttttatccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccatacccgtttttttgggctagctactagagaaagaggagaaatactagatgtatccgatggatcgtattcaacaaaaacatgctcgtcaaatagatctgctggaaaatctgacggcagttattcaggattatccaaatccagcctgtatcagggacgaaactggaaaatttattttttgcaatacgctgtttcatgagtcatttcttacacaagatcaaagtgctgaaaaatggcttctgtcgcagagagatttttgtgaattgatctctgtcacagagatggaagcatataggaatgagcatacgcatcttaatcttgtagaggatgtttttattcagaatagattctggacaatatctgtccagtcatttcttaatggacacagaaatattattctgtggcaattttatgatgctgctcatgttcgtcataaagacagttataatcaaaaaacgattgtcagtgatgatatcagaaatataatcagaagaatgagtgatgattcttctgtatcatcatatgtaaatgatgtcttttacttatatagcaccggaatcagtcataatgctatagcaagaatattaaatatatccatctccacatcaaagaaacacgcatctctgatatgcgactacttctctgtttctaataaagatgagttaattatcttactctacaataaaaagtttatttattatttatacgagaaggctatgtgtatcataaatacgcgttaatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z