##gff-version 3
##sequence-region BBa_J03103 1 2935
BBa_J03103	.	promoter	1	200	.	+	0	ID=BBa_R0010;Name=BBa_R0010
BBa_J03103	.	non_covalent_binding_site	89	126	.	+	0	ID=annotation1961223;Name=CAP binding site;Parent=BBa_R0010
BBa_J03103	.	promoter	161	166	.	+	0	ID=annotation1961225;Name=-10;Parent=BBa_R0010
BBa_J03103	.	engineered_region	1	200	.	+	0	ID=annotation1961222;Name=BBa_R0010;Parent=BBa_R0010
BBa_J03103	.	CDS	1	88	.	+	0	ID=annotation1961221;Name=end of LacI coding region (inactive);Parent=BBa_R0010
BBa_J03103	.	non_covalent_binding_site	166	200	.	+	0	ID=annotation1961226;Name=LacI binding site;Parent=BBa_R0010
BBa_J03103	.	promoter	137	142	.	+	0	ID=annotation1961224;Name=-35;Parent=BBa_R0010
BBa_J03103	.	start_codon	173	173	.	+	0	ID=annotation1961227;Name=start;Parent=BBa_R0010
BBa_J03103	.	promoter	1968	2049	.	+	0	ID=BBa_I12006;Name=BBa_I12006
BBa_J03103	.	non_covalent_binding_site	2023	2036	.	+	0	ID=annotation786365;Name=OR1 434;Parent=BBa_I12006
BBa_J03103	.	promoter	2038	2043	.	+	0	ID=annotation837228;Name=-10;Parent=BBa_I12006
BBa_J03103	.	non_covalent_binding_site	1976	1992	.	+	0	ID=annotation786500;Name=OR1 lambda;Parent=BBa_I12006
BBa_J03103	.	non_covalent_binding_site	2000	2016	.	+	0	ID=annotation786518;Name=OR2 lambda;Parent=BBa_I12006
BBa_J03103	.	promoter	2015	2020	.	+	0	ID=annotation837284;Name=-35;Parent=BBa_I12006
BBa_J03103	.	terminator	1914	1959	.	+	0	ID=BBa_B0011;Name=BBa_B0011
BBa_J03103	.	stem_loop	1926	1948	.	+	0	ID=annotation1683;Name=stem_loop;Parent=BBa_B0011
BBa_J03103	.	engineered_region	1914	1959	.	+	0	ID=annotation7019;Name=BBa_B0011;Parent=BBa_B0011
BBa_J03103	.	ribosome_entry_site	2058	2069	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_J03103	.	conserved_region	2062	2065	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_J03103	.	terminator	1865	1905	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_J03103	.	engineered_region	1865	1905	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_J03103	.	stop_codon	1898	1898	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_J03103	.	stem_loop	1872	1891	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_J03103	.	polyA_site	1892	1905	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_J03103	.	terminator	2895	2935	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_J03103	.	engineered_region	2895	2935	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_J03103	.	stop_codon	2928	2928	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_J03103	.	stem_loop	2902	2921	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_J03103	.	polyA_site	2922	2935	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_J03103	.	terminator	1010	1050	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_J03103	.	engineered_region	1010	1050	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_J03103	.	stop_codon	1043	1043	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_J03103	.	stem_loop	1017	1036	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_J03103	.	polyA_site	1037	1050	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_J03103	.	terminator	1059	1104	.	+	0	ID=BBa_B0011;Name=BBa_B0011
BBa_J03103	.	stem_loop	1071	1093	.	+	0	ID=annotation1683;Name=stem_loop;Parent=BBa_B0011
BBa_J03103	.	engineered_region	1059	1104	.	+	0	ID=annotation7019;Name=BBa_B0011;Parent=BBa_B0011
BBa_J03103	.	ribosome_entry_site	209	220	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_J03103	.	conserved_region	213	216	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_J03103	.	CDS	227	976	.	+	0	ID=BBa_C0051;Name=BBa_C0051
BBa_J03103	.	tag	938	970	.	+	0	ID=annotation23335;Name=LVA;Parent=BBa_C0051
BBa_J03103	.	CDS	230	937	.	+	0	ID=annotation23334;Name=cI lambda;Parent=BBa_C0051
BBa_J03103	.	engineered_tag	977	1001	.	+	0	ID=annotation2213991;Name=Help:Barcodes;Parent=BBa_C0051
BBa_J03103	.	ribosome_entry_site	1203	1214	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_J03103	.	conserved_region	1207	1210	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_J03103	.	CDS	2076	2798	.	+	0	ID=BBa_E0030;Name=BBa_E0030
BBa_J03103	.	promoter	1113	1194	.	+	0	ID=BBa_I12007;Name=BBa_I12007
BBa_J03103	.	non_covalent_binding_site	1145	1161	.	+	0	ID=annotation937702;Name=OR2;Parent=BBa_I12007
BBa_J03103	.	sequence_alteration	1171	1177	.	+	0	ID=annotation937704;Name=mutate to obliterate OR3;Parent=BBa_I12007
BBa_J03103	.	promoter	1183	1188	.	+	0	ID=annotation937705;Name=-10;Parent=BBa_I12007
BBa_J03103	.	promoter	1160	1165	.	+	0	ID=annotation937703;Name=-35;Parent=BBa_I12007
BBa_J03103	.	non_covalent_binding_site	1121	1137	.	+	0	ID=annotation937701;Name=OR1;Parent=BBa_I12007
BBa_J03103	.	CDS	1221	1856	.	+	0	ID=BBa_C0056;Name=BBa_C0056
BBa_J03103	.	CDS	1221	1856	.	+	0	ID=annotation1063931;Name=cI 434;Parent=BBa_C0056
BBa_J03103	.	stop_codon	1851	1856	.	+	0	ID=annotation1063930;Name=2;Parent=BBa_C0056
BBa_J03103	.	terminator	2807	2886	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_J03103	.	engineered_region	2807	2886	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_J03103	.	stem_loop	2818	2861	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
>BBa_J03103
caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagc
gggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggct
cgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagaaagaggagaaatactagatgagcacaaa
aaagaaaccattaacacaagagcagcttgaggacgcacgtcgccttaaagcaatttatgaaaaaaagaaaaatgaactt
ggcttatcccaggaatctgtcgcagacaagatggggatggggcagtcaggcgttggtgctttatttaatggcatcaatg
cattaaatgcttataacgccgcattgcttgcaaaaattctcaaagttagcgttgaagaatttagcccttcaatcgccag
agaaatctacgagatgtatgaagcggttagtatgcagccgtcacttagaagtgagtatgagtaccctgttttttctcat
gttcaggcagggatgttctcacctgagcttagaacctttaccaaaggtgatgcggagagatgggtaagcacaaccaaaa
aagccagtgattctgcattctggcttgaggttgaaggtaattccatgaccgcaccaacaggctccaagccaagctttcc
tgacggaatgttaattctcgttgaccctgagcaggctgttgagccaggtgatttctgcatagccagacttgggggtgat
gagtttaccttcaagaaactgatcagggatagcggtcaggtgtttttacaaccactaaacccacagtacccaatgatcc
catgcaatgagagttgttccgttgtggggaaagttatcgctagtcagtggcctgaagagacgtttggcgctgcaaacga
cgaaaactacgctttagtagcttaataacgctgatagtgctagtgtagatcgctactagagtcacactggctcaccttc
gggtgggcctttctgcgtttatatactagagagagaatataaaaagccagattattaatccggcttttttattatttta
ctagaggcaaccattatcaccgccagaggtaaaatagtcaacacgcacggtgttagatatttataaatagtggtgatag
atttaacgttactagagaaagaggagaaatactagatgagtatttcttccagggtaaaaagcaaaagaatccagcttgg
acttaaccaggctgaacttgctcaaaaggtggggactacccagcagtctatagagcagctcgaaaacggtaaaactaag
cgaccacgctttttaccagaacttgcgtcagctcttggcgtaagtgttgactggctgctcaatggcacctctgattcga
atgttagatttgttgggcacgttgagcccaaagggaaatatccattgattagcatggttagagctggttcgtggtgtga
agcttgtgaaccctacgatatcaaggacattgatgaatggtatgacagtgacgttaacttattaggcaatggattctgg
ctgaaggttgaaggtgattccatgacctcacctgtaggtcaaagcatccctgaaggtcatatggtgttagtagatactg
gacgggagccagtgaatggaagccttgttgtagccaaactgactgacgcgaacgaagcaacattcaagaaactggtcat
agatggcggtcagaagtacctgaaaggcctgaatccttcatggcctatgactcctatcaacggaaactgcaagattatc
ggtgttgtcgtggaagcgagggtaaaattcgtataataatactagagtcacactggctcaccttcgggtgggcctttct
gcgtttatatactagagagagaatataaaaagccagattattaatccggcttttttattattttactagaggcaaccat
tatcaccgccagaggtaaaatagtcaacacgcacggtgttagatattacaaactttcttgtatagatttaacgttacta
gagaaagaggagaaatactagatggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctgg
acggcgacgtaaacggccacaagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaa
gttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccttcggctacggcctgcaatgcttc
gcccgctaccccgaccacatgaagctgcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcacca
tcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcga
gctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctggagtacaactacaacagccacaacgtc
tatatcatggccgacaagcagaagaacggcatcaaggtgaacttcaagatccgccacaacatcgaggacggcagcgtgc
agctcgccgaccactaccagcagaacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagcta
ccagtccgccctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatc
actctcggcatggacgagctgtacaagtaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagac
tgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctt
tctgcgtttata