BBa_J03210
1
BBa_J03210
malE [Maltose-binding Protein]
2006-08-07T11:00:00Z
2015-08-31T04:08:13Z
Swedish Centre for Bioprocess technology, Stockholm
Ref: Larson, G.(2004) "Solubility and proteolysis of the Zb-malE and Zb-malE31 proteins during overproduction in Escherichia coli."
Biotechnology & Bioengineering Volume 90, Issue 2, Pages 239-247
malE encodes the Maltose-binding Protein, essential for both transport and chemotaxis. It resides in the periplasm.
false
false
_15_
0
740
15
Not in stock
true
The sequence information was acquired from Genbank and the physical DNA shipped from the Larson Lab, Stockholm. PCR was used to produce BioBrick
false
James Brown
annotation1894080
1
MBP
range1894080
1
1
341
annotation1894079
1
Start codon
range1894079
1
1
3
BBa_J03211
1
BBa_J03211
MBP Output Module
2006-08-07T11:00:00Z
2015-08-31T04:08:13Z
Constructed from Standard Registry rbs and terminators. Coupled with malE gene taken from previous BioBrick (see BBa_J03210)
This device takes a standard PoPS input and produces the malE-encoded Maltose-bindin protein. This in turn can initiate maltose chemotaxis when used in the appropriate chassis (malE KO) and enbale the cell to metabolise maltose, when previously it was blind to and unable to ttransport it into the Cytoplasm.
false
false
_15_
0
740
15
Not in stock
false
This device is only useful when used in a malE knock-out strain, thus lacking the essential MBP.
false
James Brown
component1894191
1
BBa_B0030
component1894196
1
BBa_B0010
component1894195
1
BBa_J03210
component1894198
1
BBa_B0012
annotation1894198
1
BBa_B0012
range1894198
1
460
500
annotation1894196
1
BBa_B0010
range1894196
1
372
451
annotation1894191
1
BBa_B0030
range1894191
1
1
15
annotation1894195
1
BBa_J03210
range1894195
1
22
363
BBa_B0030
1
BBa_B0030
RBS.1 (strong) -- modified from R. Weiss
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Released HQ 2013
Strong RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0032</bb_part>, <bb_part>BBa_B0033</bb_part>.
false
true
_44_46_
0
24
7
In stock
false
Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("orig" in figure 4-14 of Ron Weiss thesis). <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS.
Contact info <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a>
true
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
annotation7025
1
BBa_B0030
range7025
1
1
15
annotation1702
1
RBS
range1702
1
8
12
annotation1701
1
RBS-1\Strong
range1701
1
1
15
BBa_B0010
1
BBa_B0010
T1 from E. coli rrnB
2003-11-19T12:00:00Z
2015-08-31T04:07:20Z
Transcriptional terminator consisting of a 64 bp stem-loop.
false
false
_1_
0
24
7
In stock
false
true
Randy Rettberg
annotation4184
1
stem_loop
range4184
1
12
55
annotation7018
1
BBa_B0010
range7018
1
1
80
BBa_B0012
1
BBa_B0012
TE from coliphageT7
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
Released HQ 2013
Transcription terminator for the <i>E.coli</i> RNA polymerase.
false
false
_1_
0
24
7
In stock
false
<P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
false
Reshma Shetty
annotation1686
1
T7 TE
range1686
1
8
27
annotation1687
1
stop
range1687
1
34
34
annotation1690
1
polya
range1690
1
28
41
annotation7020
1
BBa_B0012
range7020
1
1
41
BBa_B0010_sequence
1
ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
BBa_J03210_sequence
1
atgaaaataaaaacaggtgcacgcatcctcgcattatccgcattaacgacgatgatgttttccgcctcggctctcgccaaaatcgaagaaggtaaactggtaatctggattaacggcgataaaggctataacggtctcgctgaagtcggtaagaaattcgagaaagataccggaattaaagtcaccgttgagcatccggataaactggaagagaaattcccacaggttgcggcaactggcgatggccctgacattatcttctgggcacacgaccgctttggtggctacgctcaatctggcctgttggctgaaatcaccccggacaaagcgttccaggacaag
BBa_B0030_sequence
1
attaaagaggagaaa
BBa_B0012_sequence
1
tcacactggctcaccttcgggtgggcctttctgcgtttata
BBa_J03211_sequence
1
attaaagaggagaaatactagatgaaaataaaaacaggtgcacgcatcctcgcattatccgcattaacgacgatgatgttttccgcctcggctctcgccaaaatcgaagaaggtaaactggtaatctggattaacggcgataaaggctataacggtctcgctgaagtcggtaagaaattcgagaaagataccggaattaaagtcaccgttgagcatccggataaactggaagagaaattcccacaggttgcggcaactggcgatggccctgacattatcttctgggcacacgaccgctttggtggctacgctcaatctggcctgttggctgaaatcaccccggacaaagcgttccaggacaagtactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
James Alastair McLaughlin
Chris J. Myers
2017-03-06T15:00:00.000Z