LOCUS       BBa_J04086              1632 bp    DNA     linear       14-May-2024
DEFINITION  "Off" Antiswitch and target EYFP in the same plasmid
ACCESSION   BBa_J04086
VERSION     BBa_J04086.1  
FEATURES             Location/Qualifiers
     promoter        1..200
                     /label=R0010
     misc_feature    201..253
                     /label=Hammerhead Ribozyme
     misc_feature    254..327
                     /label=Antiswitch
     misc_feature    328..378
                     /label=Hammerhead Ribozyme
     misc_feature    379..507
                     /label=B0015
     promoter        508..707
                     /label=R0010
     CDS             708..1495
                     /label=EYFP
     misc_feature    1496..1632
                     /label=B0015
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca ggatcccaag ctgatgagtc cgtgaggacg aaacggtagg
      241 acttcctacc gtccttgctc accatctacc actagatggt gagcaaggat accagcatcg
      301 tcttgatgcc cttggcagcc ttgctcaacc ggagtcgact ccggtctgat gagtccgtga
      361 ggacgaaacc atctcgagcc aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg
      421 cctttcgttt tatctgttgt ttgtcggtga acgctctcta ctagagtcac actggctcac
      481 cttcgggtgg gcctttctgc gtttatacaa tacgcaaacc gcctctcccc gcgcgttggc
      541 cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca
      601 acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc
      661 cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacatac tagagaaaga
      721 ggagaaatac tagatggtga gcaagggcga ggagctgttc accggggtgg tgcccatcct
      781 ggtcgagctg gacggcgacg taaacggcca caagttcagc gtgtccggcg agggcgaggg
      841 cgatgccacc tacggcaagc tgaccctgaa gttcatctgc accaccggca agctgcccgt
      901 gccctggccc accctcgtga ccaccttcgg ctacggcctg caatgcttcg cccgctaccc
      961 cgaccacatg aagctgcacg acttcttcaa gtccgccatg cccgaaggct acgtccagga
     1021 gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc cgcgccgagg tgaagttcga
     1081 gggcgacacc ctggtgaacc gcatcgagct gaagggcatc gacttcaagg aggacggcaa
     1141 catcctgggg cacaagctgg agtacaacta caacagccac aacgtctata tcatggccga
     1201 caagcagaag aacggcatca aggtgaactt caagatccgc cacaacatcg aggacggcag
     1261 cgtgcagctc gccgaccact accagcagaa cacccccatc ggcgacggcc ccgtgctgct
     1321 gcccgacaac cactacctga gctaccagtc cgccctgagc aaagacccca acgagaagcg
     1381 cgatcacatg gtcctgctgg agttcgtgac cgccgccggg atcactctcg gcatggacga
     1441 gctgtacaag aggcctgctg caaacgacga aaactacgct ttagtagctt aataatacta
     1501 gagccaggca tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt cgttttatct
     1561 gttgtttgtc ggtgaacgct ctctactaga gtcacactgg ctcaccttcg ggtgggcctt
     1621 tctgcgttta ta
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