BBa_J04086 1 BBa_J04086 "Off" Antiswitch and target EYFP in the same plasmid 2005-12-04T12:00:00Z 2015-08-31T04:08:13Z Adaptation of antiswitch design "S8" by Bayer and Smolke; article available at http://www.nature.com/nbt/journal/v23/n3/full/nbt1069.html This is an "Off" antiswitch placed into the same plasmid as the target EYFP. Both have the same promoter. This will help to ensure a close ratio of Antiswitch to EYFP mRNA. This part was designed based on Smolke and Bayer (2005) antiswitch S8. false false _16_ 0 428 16 It's complicated false false Matt Gemberling annotation1759648 1 Hammerhead Ribozyme range1759648 1 201 253 annotation1759641 1 R0010 range1759641 1 1 200 annotation1759652 1 Antiswitch range1759652 1 254 327 annotation1759670 1 B0015 range1759670 1 1496 1632 annotation1759658 1 B0015 range1759658 1 379 507 annotation1759653 1 Hammerhead Ribozyme range1759653 1 328 378 annotation1759660 1 EYFP range1759660 1 708 1495 annotation1759659 1 R0010 range1759659 1 508 707 BBa_J04086_sequence 1 caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacaggatcccaagctgatgagtccgtgaggacgaaacggtaggacttcctaccgtccttgctcaccatctaccactagatggtgagcaaggataccagcatcgtcttgatgcccttggcagccttgctcaaccggagtcgactccggtctgatgagtccgtgaggacgaaaccatctcgagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatacaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagaaagaggagaaatactagatggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtaaacggccacaagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccttcggctacggcctgcaatgcttcgcccgctaccccgaccacatgaagctgcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctggagtacaactacaacagccacaacgtctatatcatggccgacaagcagaagaacggcatcaaggtgaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagcagaacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagctaccagtccgccctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgtacaagaggcctgctgcaaacgacgaaaactacgctttagtagcttaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z