LOCUS       BBa_J04381              1262 bp    DNA     linear       11-Dec-2025
DEFINITION  Terminator placed in front of I6060 (EYFP)
ACCESSION   BBa_J04381
VERSION     BBa_J04381.1  
FEATURES             Location/Qualifiers
     stem_loop       12..55
                     /label=stem_loop
     misc_feature    1..80
                     /label=BBa_B0010
     terminator      1..80
                     /label=BBa_B0010
     stem_loop       96..115
                     /label=T7 TE
     misc_feature    122..122
                     /label=stop
     polyA_site      116..129
                     /label=polya
     misc_feature    89..129
                     /label=BBa_B0012
     terminator      89..129
                     /label=BBa_B0012
     CDS             138..225
                     /label=end of LacI coding region (inactive)
     misc_feature    138..337
                     /label=BBa_R0010
     misc_feature    226..263
                     /label=CAP binding site
     promoter        274..279
                     /label=-35
     promoter        298..303
                     /label=-10
     misc_feature    303..337
                     /label=LacI binding site
     misc_feature    310..310
                     /label=start
     promoter        138..337
                     /label=BBa_R0010
     misc_feature    350..353
                     /label=conserved
     RBS             346..357
                     /label=BBa_B0034
     CDS             364..1125
                     /label=YFP (LVA)
     misc_feature    1120..1125
                     /label=2
     misc_feature    1081..1119
                     /label=SsrA
     misc_feature    605..605
                     /label=A (Q->L)
     misc_feature    364..1125
                     /label=BBa_E0032
     misc_feature    364..1125
                     /label=BBa_E0032
     stem_loop       1145..1188
                     /label=stem_loop
     misc_feature    1134..1213
                     /label=BBa_B0010
     terminator      1134..1213
                     /label=BBa_B0010
     stem_loop       1229..1248
                     /label=T7 TE
     misc_feature    1255..1255
                     /label=stop
     polyA_site      1249..1262
                     /label=polya
     misc_feature    1222..1262
                     /label=BBa_B0012
     terminator      1222..1262
                     /label=BBa_B0012
ORIGIN
        1 ccaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt tttatctgtt
       61 gtttgtcggt gaacgctctc tactagagtc acactggctc accttcgggt gggcctttct
      121 gcgtttatat actagagcaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta
      181 atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa
      241 tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc cggctcgtat
      301 gttgtgtgga attgtgagcg gataacaatt tcacacatac tagagaaaga ggagaaatac
      361 tagatggtga gcaagggcga ggagctgttc accggggtgg tgcccatcct ggtcgagctg
      421 gacggcgacg taaacggcca caagttcagc gtgtccggcg agggcgaggg cgatgccacc
      481 tacggcaagc tgaccctgaa gttcatctgc accaccggca agctgcccgt gccctggccc
      541 accctcgtga ccaccttcgg ctacggcctg caatgcttcg cccgctaccc cgaccacatg
      601 aagctgcacg acttcttcaa gtccgccatg cccgaaggct acgtccagga gcgcaccatc
      661 ttcttcaagg acgacggcaa ctacaagacc cgcgccgagg tgaagttcga gggcgacacc
      721 ctggtgaacc gcatcgagct gaagggcatc gacttcaagg aggacggcaa catcctgggg
      781 cacaagctgg agtacaacta caacagccac aacgtctata tcatggccga caagcagaag
      841 aacggcatca aggtgaactt caagatccgc cacaacatcg aggacggcag cgtgcagctc
      901 gccgaccact accagcagaa cacccccatc ggcgacggcc ccgtgctgct gcccgacaac
      961 cactacctga gctaccagtc cgccctgagc aaagacccca acgagaagcg cgatcacatg
     1021 gtcctgctgg agttcgtgac cgccgccggg atcactctcg gcatggacga gctgtacaag
     1081 aggcctgctg caaacgacga aaactacgct ttagtagctt aataatacta gagccaggca
     1141 tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt cgttttatct gttgtttgtc
     1201 ggtgaacgct ctctactaga gtcacactgg ctcaccttcg ggtgggcctt tctgcgttta
     1261 ta
//