##gff-version 3
##sequence-region BBa_J04381 1 1262
BBa_J04381	.	engineered_region	364	1125	.	+	0	ID=BBa_E0032;Name=BBa_E0032
BBa_J04381	.	tag	1081	1119	.	+	0	ID=annotation2160;Name=SsrA;Parent=BBa_E0032
BBa_J04381	.	stop_codon	1120	1125	.	+	0	ID=annotation2159;Name=2;Parent=BBa_E0032
BBa_J04381	.	sequence_alteration	605	605	.	+	0	ID=annotation2161;Name=A (Q->L);Parent=BBa_E0032
BBa_J04381	.	engineered_region	364	1125	.	+	0	ID=annotation7041;Name=BBa_E0032;Parent=BBa_E0032
BBa_J04381	.	CDS	364	1125	.	+	0	ID=annotation2156;Name=YFP (LVA);Parent=BBa_E0032
BBa_J04381	.	terminator	1222	1262	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_J04381	.	engineered_region	1222	1262	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_J04381	.	polyA_site	1249	1262	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_J04381	.	stem_loop	1229	1248	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_J04381	.	stop_codon	1255	1255	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_J04381	.	terminator	1	80	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_J04381	.	engineered_region	1	80	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_J04381	.	stem_loop	12	55	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_J04381	.	promoter	138	337	.	+	0	ID=BBa_R0010;Name=BBa_R0010
BBa_J04381	.	CDS	138	225	.	+	0	ID=annotation1961221;Name=end of LacI coding region (inactive);Parent=BBa_R0010
BBa_J04381	.	start_codon	310	310	.	+	0	ID=annotation1961227;Name=start;Parent=BBa_R0010
BBa_J04381	.	non_covalent_binding_site	303	337	.	+	0	ID=annotation1961226;Name=LacI binding site;Parent=BBa_R0010
BBa_J04381	.	promoter	298	303	.	+	0	ID=annotation1961225;Name=-10;Parent=BBa_R0010
BBa_J04381	.	promoter	274	279	.	+	0	ID=annotation1961224;Name=-35;Parent=BBa_R0010
BBa_J04381	.	non_covalent_binding_site	226	263	.	+	0	ID=annotation1961223;Name=CAP binding site;Parent=BBa_R0010
BBa_J04381	.	engineered_region	138	337	.	+	0	ID=annotation1961222;Name=BBa_R0010;Parent=BBa_R0010
BBa_J04381	.	ribosome_entry_site	346	357	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_J04381	.	conserved_region	350	353	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_J04381	.	terminator	89	129	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_J04381	.	engineered_region	89	129	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_J04381	.	polyA_site	116	129	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_J04381	.	stem_loop	96	115	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_J04381	.	stop_codon	122	122	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_J04381	.	terminator	1134	1213	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_J04381	.	engineered_region	1134	1213	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_J04381	.	stem_loop	1145	1188	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
>BBa_J04381
ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctct
ctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagcaatacgcaaaccgcctctcc
ccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaat
taatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtg
agcggataacaatttcacacatactagagaaagaggagaaatactagatggtgagcaagggcgaggagctgttcaccgg
ggtggtgcccatcctggtcgagctggacggcgacgtaaacggccacaagttcagcgtgtccggcgagggcgagggcgat
gccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgacca
ccttcggctacggcctgcaatgcttcgcccgctaccccgaccacatgaagctgcacgacttcttcaagtccgccatgcc
cgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgag
ggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctgg
agtacaactacaacagccacaacgtctatatcatggccgacaagcagaagaacggcatcaaggtgaacttcaagatccg
ccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagcagaacacccccatcggcgacggccccgtgctg
ctgcccgacaaccactacctgagctaccagtccgccctgagcaaagaccccaacgagaagcgcgatcacatggtcctgc
tggagttcgtgaccgccgccgggatcactctcggcatggacgagctgtacaagaggcctgctgcaaacgacgaaaacta
cgctttagtagcttaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttt
tatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata