##gff-version 3
##sequence-region BBa_J04451 1 1083
BBa_J04451	.	engineered_region	227	946	.	+	0	ID=BBa_J04051;Name=BBa_J04051
BBa_J04451	.	CDS	227	901	.	+	0	ID=annotation1507347;Name=mrfp1;Parent=BBa_J04051
BBa_J04451	.	stop_codon	941	946	.	+	0	ID=annotation1507863;Name=Stop;Parent=BBa_J04051
BBa_J04451	.	start_codon	227	229	.	+	0	ID=annotation1508160;Name=Start;Parent=BBa_J04051
BBa_J04451	.	tag	902	940	.	+	0	ID=annotation1507968;Name=LVA;Parent=BBa_J04051
BBa_J04451	.	ribosome_entry_site	209	220	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_J04451	.	conserved_region	213	216	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_J04451	.	promoter	1	200	.	+	0	ID=BBa_R0010;Name=BBa_R0010
BBa_J04451	.	non_covalent_binding_site	89	126	.	+	0	ID=annotation1961223;Name=CAP binding site;Parent=BBa_R0010
BBa_J04451	.	non_covalent_binding_site	166	200	.	+	0	ID=annotation1961226;Name=LacI binding site;Parent=BBa_R0010
BBa_J04451	.	promoter	137	142	.	+	0	ID=annotation1961224;Name=-35;Parent=BBa_R0010
BBa_J04451	.	engineered_region	1	200	.	+	0	ID=annotation1961222;Name=BBa_R0010;Parent=BBa_R0010
BBa_J04451	.	promoter	161	166	.	+	0	ID=annotation1961225;Name=-10;Parent=BBa_R0010
BBa_J04451	.	start_codon	173	173	.	+	0	ID=annotation1961227;Name=start;Parent=BBa_R0010
BBa_J04451	.	CDS	1	88	.	+	0	ID=annotation1961221;Name=end of LacI coding region (inactive);Parent=BBa_R0010
BBa_J04451	.	terminator	1043	1083	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_J04451	.	stop_codon	1076	1076	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_J04451	.	polyA_site	1070	1083	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_J04451	.	stem_loop	1050	1069	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_J04451	.	engineered_region	1043	1083	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_J04451	.	terminator	955	1034	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_J04451	.	engineered_region	955	1034	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_J04451	.	stem_loop	966	1009	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
>BBa_J04451
caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagc
gggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggct
cgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagaaagaggagaaatactagatggcttcctc
cgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaa
ggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcg
cttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacct
gaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccag
gactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggtta
tgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaa
aatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcag
ctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacg
aacgtgctgaaggtcgtcactccaccggtgctaggcctgctgcaaacgacgaaaactacgctttagtagcttaataata
ctagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaa
cgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata