LOCUS       BBa_J04481              1105 bp    DNA     linear       22-Dec-2024
DEFINITION  BBa_R0010 Promoter (RBS+) with BBa_J04671 (EYFP, RBS-, LVA+, B0015)
ACCESSION   BBa_J04481
VERSION     BBa_J04481.1  
FEATURES             Location/Qualifiers
     CDS             1..88
                     /label=end of LacI coding region (inactive)
     misc_feature    1..200
                     /label=BBa_R0010
     misc_feature    89..126
                     /label=CAP binding site
     promoter        137..142
                     /label=-35
     promoter        161..166
                     /label=-10
     misc_feature    166..200
                     /label=LacI binding site
     misc_feature    173..173
                     /label=start
     promoter        1..200
                     /label=BBa_R0010
     CDS             207..968
                     /label=YFP (LVA)
     misc_feature    963..968
                     /label=2
     misc_feature    924..962
                     /label=SsrA
     misc_feature    448..448
                     /label=A (Q->L)
     misc_feature    207..968
                     /label=BBa_E0032
     misc_feature    207..968
                     /label=BBa_E0032
     stem_loop       988..1031
                     /label=stem_loop
     misc_feature    977..1056
                     /label=BBa_B0010
     terminator      977..1056
                     /label=BBa_B0010
     stem_loop       1072..1091
                     /label=T7 TE
     misc_feature    1098..1098
                     /label=stop
     polyA_site      1092..1105
                     /label=polya
     misc_feature    1065..1105
                     /label=BBa_B0012
     terminator      1065..1105
                     /label=BBa_B0012
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagatgg tgagcaaggg cgaggagctg ttcaccgggg
      241 tggtgcccat cctggtcgag ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg
      301 gcgagggcga gggcgatgcc acctacggca agctgaccct gaagttcatc tgcaccaccg
      361 gcaagctgcc cgtgccctgg cccaccctcg tgaccacctt cggctacggc ctgcaatgct
      421 tcgcccgcta ccccgaccac atgaagctgc acgacttctt caagtccgcc atgcccgaag
      481 gctacgtcca ggagcgcacc atcttcttca aggacgacgg caactacaag acccgcgccg
      541 aggtgaagtt cgagggcgac accctggtga accgcatcga gctgaagggc atcgacttca
      601 aggaggacgg caacatcctg gggcacaagc tggagtacaa ctacaacagc cacaacgtct
      661 atatcatggc cgacaagcag aagaacggca tcaaggtgaa cttcaagatc cgccacaaca
      721 tcgaggacgg cagcgtgcag ctcgccgacc actaccagca gaacaccccc atcggcgacg
      781 gccccgtgct gctgcccgac aaccactacc tgagctacca gtccgccctg agcaaagacc
      841 ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc gggatcactc
      901 tcggcatgga cgagctgtac aagaggcctg ctgcaaacga cgaaaactac gctttagtag
      961 cttaataata ctagagccag gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc
     1021 tttcgtttta tctgttgttt gtcggtgaac gctctctact agagtcacac tggctcacct
     1081 tcgggtgggc ctttctgcgt ttata
//