BBa_J04605
1
BBa_J04605
R0010 with Anti-Sense Theophillline Induced Antiswitch ("On Switch")
2005-09-14T11:00:00Z
2015-08-31T04:08:14Z
This part contains the R0010 Promoter region attached to an anti-sense antiswitch sequence for the regulation of EYFP This Promoter and Antiswitch construct is designed so that it can be attached to any form of the EYFP coding region. The purpose of this piece is to regulate the expression of YFP in the presence of theophylline. In this construct, the presence of theophylline causes the antiswitch, which is initially bound to the YFP coding region (preventing expression), to release and fold up on itself allowing for EYFP expression.
false
false
_16_
0
435
16
Not in stock
false
Adaptation of antiswitch design "S8" by Bayer and Smolke; article available at http://www.nature.com/nbt/journal/v23/n3/full/nbt1069.html
false
Oscar Hernandez and Matt Gemberling
annotation1691971
1
Hammerhead Riobozyme #2
range1691971
1
309
359
annotation1691967
1
Antiswitch
range1691967
1
254
308
annotation1691966
1
Hammerhead Riobozyme #1
range1691966
1
201
253
annotation1691995
1
Antisense Binding Sequence
range1691995
1
254
268
annotation1691965
1
R0010 Promoter Region
range1691965
1
1
200
BBa_J04605_sequence
1
caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacaggatcccaagctgatgagtccgtgaggacgaaacggtaggacttcctaccgtccttgctcaccatctagtaccagcatcgtcttgatgcccttggcagctagatggtaccggagtcgactccggtctgatgagtccgtgaggacgaaaccatctcgag
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z