LOCUS       BBa_J04771              1163 bp    DNA     linear       22-Dec-2024
DEFINITION  BBa_J04700 (ForAptRibo) Riboswitch with BBa_J04671 (EYFP, RBS-, 
            LVA+, B0015)
ACCESSION   BBa_J04771
VERSION     BBa_J04771.1  
FEATURES             Location/Qualifiers
     promoter        1..200
                     /label=Promoter (R0010)
     misc_feature    201..238
                     /label=Aptamer (J04705)
     misc_feature    239..246
                     /label=8 bp from Gallivan paper
     RBS             247..258
                     /label=RBS (B0034)
     promoter        1..258
                     /label=BBa_J04700
     CDS             265..1026
                     /label=YFP (LVA)
     misc_feature    1021..1026
                     /label=2
     misc_feature    982..1020
                     /label=SsrA
     misc_feature    506..506
                     /label=A (Q->L)
     misc_feature    265..1026
                     /label=BBa_E0032
     misc_feature    265..1026
                     /label=BBa_E0032
     stem_loop       1046..1089
                     /label=stem_loop
     misc_feature    1035..1114
                     /label=BBa_B0010
     terminator      1035..1114
                     /label=BBa_B0010
     stem_loop       1130..1149
                     /label=T7 TE
     misc_feature    1156..1156
                     /label=stop
     polyA_site      1150..1163
                     /label=polya
     misc_feature    1123..1163
                     /label=BBa_B0012
     terminator      1123..1163
                     /label=BBa_B0012
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca ggtgatacca gcatcgtctt gatgcccttg gcagcaccta
      241 taaaagaaag aggagaaata ctagatggtg agcaagggcg aggagctgtt caccggggtg
      301 gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc acaagttcag cgtgtccggc
      361 gagggcgagg gcgatgccac ctacggcaag ctgaccctga agttcatctg caccaccggc
      421 aagctgcccg tgccctggcc caccctcgtg accaccttcg gctacggcct gcaatgcttc
      481 gcccgctacc ccgaccacat gaagctgcac gacttcttca agtccgccat gcccgaaggc
      541 tacgtccagg agcgcaccat cttcttcaag gacgacggca actacaagac ccgcgccgag
      601 gtgaagttcg agggcgacac cctggtgaac cgcatcgagc tgaagggcat cgacttcaag
      661 gaggacggca acatcctggg gcacaagctg gagtacaact acaacagcca caacgtctat
      721 atcatggccg acaagcagaa gaacggcatc aaggtgaact tcaagatccg ccacaacatc
      781 gaggacggca gcgtgcagct cgccgaccac taccagcaga acacccccat cggcgacggc
      841 cccgtgctgc tgcccgacaa ccactacctg agctaccagt ccgccctgag caaagacccc
      901 aacgagaagc gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg gatcactctc
      961 ggcatggacg agctgtacaa gaggcctgct gcaaacgacg aaaactacgc tttagtagct
     1021 taataatact agagccaggc atcaaataaa acgaaaggct cagtcgaaag actgggcctt
     1081 tcgttttatc tgttgtttgt cggtgaacgc tctctactag agtcacactg gctcaccttc
     1141 gggtgggcct ttctgcgttt ata
//