##gff-version 3
##sequence-region BBa_J04971 1 1163
BBa_J04971	.	promoter	1	258	.	+	0	ID=BBa_J04900;Name=BBa_J04900
BBa_J04971	.	ribosome_entry_site	209	220	.	+	0	ID=annotation1730507;Name=RBS (B0034);Parent=BBa_J04900
BBa_J04971	.	sequence_feature	221	258	.	+	0	ID=annotation1730508;Name=Aptamer (J04705);Parent=BBa_J04900
BBa_J04971	.	sequence_feature	201	208	.	+	0	ID=annotation1730506;Name=8 bp from Gallivan paper?;Parent=BBa_J04900
BBa_J04971	.	promoter	1	200	.	+	0	ID=annotation1730505;Name=R0010;Parent=BBa_J04900
BBa_J04971	.	engineered_region	265	1026	.	+	0	ID=BBa_E0032;Name=BBa_E0032
BBa_J04971	.	sequence_alteration	506	506	.	+	0	ID=annotation2161;Name=A (Q->L);Parent=BBa_E0032
BBa_J04971	.	engineered_region	265	1026	.	+	0	ID=annotation7041;Name=BBa_E0032;Parent=BBa_E0032
BBa_J04971	.	stop_codon	1021	1026	.	+	0	ID=annotation2159;Name=2;Parent=BBa_E0032
BBa_J04971	.	tag	982	1020	.	+	0	ID=annotation2160;Name=SsrA;Parent=BBa_E0032
BBa_J04971	.	CDS	265	1026	.	+	0	ID=annotation2156;Name=YFP (LVA);Parent=BBa_E0032
BBa_J04971	.	terminator	1123	1163	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_J04971	.	stem_loop	1130	1149	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_J04971	.	polyA_site	1150	1163	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_J04971	.	stop_codon	1156	1156	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_J04971	.	engineered_region	1123	1163	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_J04971	.	terminator	1035	1114	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_J04971	.	stem_loop	1046	1089	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_J04971	.	engineered_region	1035	1114	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
>BBa_J04971
caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagc
gggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggct
cgtatgttgtgtggaattgtgagcggataacaatttcacacatataaaagaaagaggagaaaggtgataccagcatcgt
cttgatgcccttggcagcacctactagatggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcg
agctggacggcgacgtaaacggccacaagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgac
cctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccttcggctacggcctgcaa
tgcttcgcccgctaccccgaccacatgaagctgcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagc
gcaccatcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccg
catcgagctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctggagtacaactacaacagccac
aacgtctatatcatggccgacaagcagaagaacggcatcaaggtgaacttcaagatccgccacaacatcgaggacggca
gcgtgcagctcgccgaccactaccagcagaacacccccatcggcgacggccccgtgctgctgcccgacaaccactacct
gagctaccagtccgccctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgcc
gggatcactctcggcatggacgagctgtacaagaggcctgctgcaaacgacgaaaactacgctttagtagcttaataat
actagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtga
acgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata