LOCUS       BBa_J05000              2376 bp    DNA     linear       27-Dec-2024
DEFINITION  Zinc Finger Protein Tester
ACCESSION   BBa_J05000
VERSION     BBa_J05000.1  
FEATURES             Location/Qualifiers
     CDS             1..88
                     /label=end of LacI coding region (inactive)
     misc_feature    1..200
                     /label=BBa_R0010
     misc_feature    89..126
                     /label=CAP binding site
     promoter        137..142
                     /label=-35
     promoter        161..166
                     /label=-10
     misc_feature    166..200
                     /label=LacI binding site
     misc_feature    173..173
                     /label=start
     promoter        1..200
                     /label=BBa_R0010
     misc_feature    213..216
                     /label=conserved
     RBS             209..220
                     /label=BBa_B0034
     CDS             227..535
                     /label=BBa_J05100
     misc_feature    548..551
                     /label=conserved
     RBS             544..555
                     /label=BBa_B0034
     CDS             562..1236
                     /label=mrfp1
     misc_feature    1243..1267
                     /label=Help:Barcodes
     CDS             562..1242
                     /label=BBa_E1010
     stem_loop       1287..1330
                     /label=stem_loop
     misc_feature    1276..1355
                     /label=BBa_B0010
     terminator      1276..1355
                     /label=BBa_B0010
     stem_loop       1371..1390
                     /label=T7 TE
     misc_feature    1397..1397
                     /label=stop
     polyA_site      1391..1404
                     /label=polya
     misc_feature    1364..1404
                     /label=BBa_B0012
     terminator      1364..1404
                     /label=BBa_B0012
     misc_feature    544..1404
                     /label=BBa_I13507
     promoter        1450..1455
                     /label=-10
     promoter        1427..1432
                     /label=-35
     misc_feature    1437..1453
                     /label=OR1
     misc_feature    1413..1429
                     /label=OR2
     misc_feature    1413..1461
                     /label=BBa_R0051
     promoter        1413..1461
                     /label=BBa_R0051
     promoter        1470..1493
                     /label=BBa_J05211
     misc_feature    1506..1509
                     /label=conserved
     RBS             1502..1513
                     /label=BBa_B0034
     CDS             1520..2239
                     /label=GFP protein
     CDS             1520..2239
                     /label=BBa_E0040
     stem_loop       2259..2302
                     /label=stem_loop
     misc_feature    2248..2327
                     /label=BBa_B0010
     terminator      2248..2327
                     /label=BBa_B0010
     stem_loop       2343..2362
                     /label=T7 TE
     misc_feature    2369..2369
                     /label=stop
     polyA_site      2363..2376
                     /label=polya
     misc_feature    2336..2376
                     /label=BBa_B0012
     terminator      2336..2376
                     /label=BBa_B0012
     misc_feature    1502..2376
                     /label=BBa_I13504
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagagaa agaggagaaa tactagatgg cgcaggcggc
      241 gctggaaccg aaagaaaaac cgtatgcgtg cccggaatgc ggcaaaagct ttagcgatcc
      301 gggcaacctg gtgcgccatc agcgcaccca taccggcgaa aaaccgtata aatgcccgga
      361 atgcggcaaa agctttagcc gcagcgataa actggtgcgc catcagcgca cccataccgg
      421 cgaaaaaccg tataaatgcc cggaatgcgg caaaagcttt agccagcgcg cgcatctgga
      481 acgccatcag cgcacccata ccggcaaaaa aaccagcggc caggcgggct aataatacta
      541 gagaaagagg agaaatacta gatggcttcc tccgaagacg ttatcaaaga gttcatgcgt
      601 ttcaaagttc gtatggaagg ttccgttaac ggtcacgagt tcgaaatcga aggtgaaggt
      661 gaaggtcgtc cgtacgaagg tacccagacc gctaaactga aagttaccaa aggtggtccg
      721 ctgccgttcg cttgggacat cctgtccccg cagttccagt acggttccaa agcttacgtt
      781 aaacacccgg ctgacatccc ggactacctg aaactgtcct tcccggaagg tttcaaatgg
      841 gaacgtgtta tgaacttcga agacggtggt gttgttaccg ttacccagga ctcctccctg
      901 caagacggtg agttcatcta caaagttaaa ctgcgtggta ccaacttccc gtccgacggt
      961 ccggttatgc agaaaaaaac catgggttgg gaagcttcca ccgaacgtat gtacccggaa
     1021 gacggtgctc tgaaaggtga aatcaaaatg cgtctgaaac tgaaagacgg tggtcactac
     1081 gacgctgaag ttaaaaccac ctacatggct aaaaaaccgg ttcagctgcc gggtgcttac
     1141 aaaaccgaca tcaaactgga catcacctcc cacaacgaag actacaccat cgttgaacag
     1201 tacgaacgtg ctgaaggtcg tcactccacc ggtgcttaat aacgctgata gtgctagtgt
     1261 agatcgctac tagagccagg catcaaataa aacgaaaggc tcagtcgaaa gactgggcct
     1321 ttcgttttat ctgttgtttg tcggtgaacg ctctctacta gagtcacact ggctcacctt
     1381 cgggtgggcc tttctgcgtt tatatactag agtaacaccg tgcgtgttga ctattttacc
     1441 tctggcggtg ataatggttg ctactagagg gaggcggggc gcggggggcg aggtactaga
     1501 gaaagaggag aaatactaga tgcgtaaagg agaagaactt ttcactggag ttgtcccaat
     1561 tcttgttgaa ttagatggtg atgttaatgg gcacaaattt tctgtcagtg gagagggtga
     1621 aggtgatgca acatacggaa aacttaccct taaatttatt tgcactactg gaaaactacc
     1681 tgttccatgg ccaacacttg tcactacttt cggttatggt gttcaatgct ttgcgagata
     1741 cccagatcat atgaaacagc atgacttttt caagagtgcc atgcccgaag gttatgtaca
     1801 ggaaagaact atatttttca aagatgacgg gaactacaag acacgtgctg aagtcaagtt
     1861 tgaaggtgat acccttgtta atagaatcga gttaaaaggt attgatttta aagaagatgg
     1921 aaacattctt ggacacaaat tggaatacaa ctataactca cacaatgtat acatcatggc
     1981 agacaaacaa aagaatggaa tcaaagttaa cttcaaaatt agacacaaca ttgaagatgg
     2041 aagcgttcaa ctagcagacc attatcaaca aaatactcca attggcgatg gccctgtcct
     2101 tttaccagac aaccattacc tgtccacaca atctgccctt tcgaaagatc ccaacgaaaa
     2161 gagagaccac atggtccttc ttgagtttgt aacagctgct gggattacac atggcatgga
     2221 tgaactatac aaataataat actagagcca ggcatcaaat aaaacgaaag gctcagtcga
     2281 aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa cgctctctac tagagtcaca
     2341 ctggctcacc ttcgggtggg cctttctgcg tttata
//