LOCUS BBa_J05030 4385 bp DNA linear 23-Dec-2024 DEFINITION Tester? ACCESSION BBa_J05030 VERSION BBa_J05030.1 FEATURES Location/Qualifiers stem_loop 12..55 /label=stem_loop misc_feature 1..80 /label=BBa_B0010 terminator 1..80 /label=BBa_B0010 stem_loop 96..115 /label=T7 TE misc_feature 122..122 /label=stop polyA_site 116..129 /label=polya misc_feature 89..129 /label=BBa_B0012 terminator 89..129 /label=BBa_B0012 CDS 138..225 /label=end of LacI coding region (inactive) misc_feature 138..337 /label=BBa_R0010 misc_feature 226..263 /label=CAP binding site promoter 274..279 /label=-35 promoter 298..303 /label=-10 misc_feature 303..337 /label=LacI binding site misc_feature 310..310 /label=start promoter 138..337 /label=BBa_R0010 misc_feature 350..353 /label=conserved RBS 346..357 /label=BBa_B0034 misc_feature 366..389 /label=BBa_J05010 misc_feature 402..405 /label=conserved RBS 398..409 /label=BBa_B0034 CDS 416..1090 /label=mrfp1 misc_feature 1097..1121 /label=Help:Barcodes CDS 416..1096 /label=BBa_E1010 stem_loop 1141..1184 /label=stem_loop misc_feature 1130..1209 /label=BBa_B0010 terminator 1130..1209 /label=BBa_B0010 stem_loop 1225..1244 /label=T7 TE misc_feature 1251..1251 /label=stop polyA_site 1245..1258 /label=polya misc_feature 1218..1258 /label=BBa_B0012 terminator 1218..1258 /label=BBa_B0012 stem_loop 1278..1321 /label=stem_loop misc_feature 1267..1346 /label=BBa_B0010 terminator 1267..1346 /label=BBa_B0010 stem_loop 1362..1381 /label=T7 TE misc_feature 1388..1388 /label=stop polyA_site 1382..1395 /label=polya misc_feature 1355..1395 /label=BBa_B0012 terminator 1355..1395 /label=BBa_B0012 promoter 1441..1446 /label=-10 promoter 1418..1423 /label=-35 misc_feature 1428..1444 /label=OR1 misc_feature 1404..1420 /label=OR2 misc_feature 1404..1452 /label=BBa_R0051 promoter 1404..1452 /label=BBa_R0051 misc_feature 1461..1482 /label=BBa_J05020 misc_feature 1491..1526 /label=BBa_J05021 misc_feature 1539..1542 /label=conserved RBS 1535..1546 /label=BBa_B0034 CDS 1553..2272 /label=GFP protein CDS 1553..2272 /label=BBa_E0040 stem_loop 2292..2335 /label=stem_loop misc_feature 2281..2360 /label=BBa_B0010 terminator 2281..2360 /label=BBa_B0010 stem_loop 2376..2395 /label=T7 TE misc_feature 2402..2402 /label=stop polyA_site 2396..2409 /label=polya misc_feature 2369..2409 /label=BBa_B0012 terminator 2369..2409 /label=BBa_B0012 misc_feature 2422..2425 /label=conserved RBS 2418..2429 /label=BBa_B0034 CDS 2436..3110 /label=mrfp1 misc_feature 3117..3141 /label=Help:Barcodes CDS 2436..3116 /label=BBa_E1010 stem_loop 3161..3204 /label=stem_loop misc_feature 3150..3229 /label=BBa_B0010 terminator 3150..3229 /label=BBa_B0010 stem_loop 3245..3264 /label=T7 TE misc_feature 3271..3271 /label=stop polyA_site 3265..3278 /label=polya misc_feature 3238..3278 /label=BBa_B0012 terminator 3238..3278 /label=BBa_B0012 stem_loop 3298..3341 /label=stem_loop misc_feature 3287..3366 /label=BBa_B0010 terminator 3287..3366 /label=BBa_B0010 stem_loop 3382..3401 /label=T7 TE misc_feature 3408..3408 /label=stop polyA_site 3402..3415 /label=polya misc_feature 3375..3415 /label=BBa_B0012 terminator 3375..3415 /label=BBa_B0012 promoter 3461..3466 /label=-10 promoter 3438..3443 /label=-35 misc_feature 3448..3464 /label=OR1 misc_feature 3424..3440 /label=OR2 misc_feature 3424..3472 /label=BBa_R0051 promoter 3424..3472 /label=BBa_R0051 misc_feature 3481..3502 /label=BBa_J05020 misc_feature 3515..3518 /label=conserved RBS 3511..3522 /label=BBa_B0034 CDS 3529..4248 /label=GFP protein CDS 3529..4248 /label=BBa_E0040 stem_loop 4268..4311 /label=stem_loop misc_feature 4257..4336 /label=BBa_B0010 terminator 4257..4336 /label=BBa_B0010 stem_loop 4352..4371 /label=T7 TE misc_feature 4378..4378 /label=stop polyA_site 4372..4385 /label=polya misc_feature 4345..4385 /label=BBa_B0012 terminator 4345..4385 /label=BBa_B0012 ORIGIN 1 ccaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt tttatctgtt 61 gtttgtcggt gaacgctctc tactagagtc acactggctc accttcgggt gggcctttct 121 gcgtttatat actagagcaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta 181 atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa 241 tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc cggctcgtat 301 gttgtgtgga attgtgagcg gataacaatt tcacacatac tagagaaaga ggagaaatac 361 tagagggggg gggggggggg gggggggggt actagagaaa gaggagaaat actagatggc 421 ttcctccgaa gacgttatca aagagttcat gcgtttcaaa gttcgtatgg aaggttccgt 481 taacggtcac gagttcgaaa tcgaaggtga aggtgaaggt cgtccgtacg aaggtaccca 541 gaccgctaaa ctgaaagtta ccaaaggtgg tccgctgccg ttcgcttggg acatcctgtc 601 cccgcagttc cagtacggtt ccaaagctta cgttaaacac ccggctgaca tcccggacta 661 cctgaaactg tccttcccgg aaggtttcaa atgggaacgt gttatgaact tcgaagacgg 721 tggtgttgtt accgttaccc aggactcctc cctgcaagac ggtgagttca tctacaaagt 781 taaactgcgt ggtaccaact tcccgtccga cggtccggtt atgcagaaaa aaaccatggg 841 ttgggaagct tccaccgaac gtatgtaccc ggaagacggt gctctgaaag gtgaaatcaa 901 aatgcgtctg aaactgaaag acggtggtca ctacgacgct gaagttaaaa ccacctacat 961 ggctaaaaaa ccggttcagc tgccgggtgc ttacaaaacc gacatcaaac tggacatcac 1021 ctcccacaac gaagactaca ccatcgttga acagtacgaa cgtgctgaag gtcgtcactc 1081 caccggtgct taataacgct gatagtgcta gtgtagatcg ctactagagc caggcatcaa 1141 ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg 1201 aacgctctct actagagtca cactggctca ccttcgggtg ggcctttctg cgtttatata 1261 ctagagccag gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta 1321 tctgttgttt gtcggtgaac gctctctact agagtcacac tggctcacct tcgggtgggc 1381 ctttctgcgt ttatatacta gagtaacacc gtgcgtgttg actattttac ctctggcggt 1441 gataatggtt gctactagag gggggggggg gggggggggg ggtactagag gggggggggg 1501 gggggggggg gggggggggg ggggggtact agagaaagag gagaaatact agatgcgtaa 1561 aggagaagaa cttttcactg gagttgtccc aattcttgtt gaattagatg gtgatgttaa 1621 tgggcacaaa ttttctgtca gtggagaggg tgaaggtgat gcaacatacg gaaaacttac 1681 ccttaaattt atttgcacta ctggaaaact acctgttcca tggccaacac ttgtcactac 1741 tttcggttat ggtgttcaat gctttgcgag atacccagat catatgaaac agcatgactt 1801 tttcaagagt gccatgcccg aaggttatgt acaggaaaga actatatttt tcaaagatga 1861 cgggaactac aagacacgtg ctgaagtcaa gtttgaaggt gatacccttg ttaatagaat 1921 cgagttaaaa ggtattgatt ttaaagaaga tggaaacatt cttggacaca aattggaata 1981 caactataac tcacacaatg tatacatcat ggcagacaaa caaaagaatg gaatcaaagt 2041 taacttcaaa attagacaca acattgaaga tggaagcgtt caactagcag accattatca 2101 acaaaatact ccaattggcg atggccctgt ccttttacca gacaaccatt acctgtccac 2161 acaatctgcc ctttcgaaag atcccaacga aaagagagac cacatggtcc ttcttgagtt 2221 tgtaacagct gctgggatta cacatggcat ggatgaacta tacaaataat aatactagag 2281 ccaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt tttatctgtt 2341 gtttgtcggt gaacgctctc tactagagtc acactggctc accttcgggt gggcctttct 2401 gcgtttatat actagagaaa gaggagaaat actagatggc ttcctccgaa gacgttatca 2461 aagagttcat gcgtttcaaa gttcgtatgg aaggttccgt taacggtcac gagttcgaaa 2521 tcgaaggtga aggtgaaggt cgtccgtacg aaggtaccca gaccgctaaa ctgaaagtta 2581 ccaaaggtgg tccgctgccg ttcgcttggg acatcctgtc cccgcagttc cagtacggtt 2641 ccaaagctta cgttaaacac ccggctgaca tcccggacta cctgaaactg tccttcccgg 2701 aaggtttcaa atgggaacgt gttatgaact tcgaagacgg tggtgttgtt accgttaccc 2761 aggactcctc cctgcaagac ggtgagttca tctacaaagt taaactgcgt ggtaccaact 2821 tcccgtccga cggtccggtt atgcagaaaa aaaccatggg ttgggaagct tccaccgaac 2881 gtatgtaccc ggaagacggt gctctgaaag gtgaaatcaa aatgcgtctg aaactgaaag 2941 acggtggtca ctacgacgct gaagttaaaa ccacctacat ggctaaaaaa ccggttcagc 3001 tgccgggtgc ttacaaaacc gacatcaaac tggacatcac ctcccacaac gaagactaca 3061 ccatcgttga acagtacgaa cgtgctgaag gtcgtcactc caccggtgct taataacgct 3121 gatagtgcta gtgtagatcg ctactagagc caggcatcaa ataaaacgaa aggctcagtc 3181 gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctct actagagtca 3241 cactggctca ccttcgggtg ggcctttctg cgtttatata ctagagccag gcatcaaata 3301 aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta tctgttgttt gtcggtgaac 3361 gctctctact agagtcacac tggctcacct tcgggtgggc ctttctgcgt ttatatacta 3421 gagtaacacc gtgcgtgttg actattttac ctctggcggt gataatggtt gctactagag 3481 gggggggggg gggggggggg ggtactagag aaagaggaga aatactagat gcgtaaagga 3541 gaagaacttt tcactggagt tgtcccaatt cttgttgaat tagatggtga tgttaatggg 3601 cacaaatttt ctgtcagtgg agagggtgaa ggtgatgcaa catacggaaa acttaccctt 3661 aaatttattt gcactactgg aaaactacct gttccatggc caacacttgt cactactttc 3721 ggttatggtg ttcaatgctt tgcgagatac ccagatcata tgaaacagca tgactttttc 3781 aagagtgcca tgcccgaagg ttatgtacag gaaagaacta tatttttcaa agatgacggg 3841 aactacaaga cacgtgctga agtcaagttt gaaggtgata cccttgttaa tagaatcgag 3901 ttaaaaggta ttgattttaa agaagatgga aacattcttg gacacaaatt ggaatacaac 3961 tataactcac acaatgtata catcatggca gacaaacaaa agaatggaat caaagttaac 4021 ttcaaaatta gacacaacat tgaagatgga agcgttcaac tagcagacca ttatcaacaa 4081 aatactccaa ttggcgatgg ccctgtcctt ttaccagaca accattacct gtccacacaa 4141 tctgcccttt cgaaagatcc caacgaaaag agagaccaca tggtccttct tgagtttgta 4201 acagctgctg ggattacaca tggcatggat gaactataca aataataata ctagagccag 4261 gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta tctgttgttt 4321 gtcggtgaac gctctctact agagtcacac tggctcacct tcgggtgggc ctttctgcgt 4381 ttata //