##gff-version 3
##sequence-region BBa_J05030 1 4385
BBa_J05030	.	promoter	3424	3472	.	+	0	ID=BBa_R0051;Name=BBa_R0051
BBa_J05030	.	non_covalent_binding_site	3448	3464	.	+	0	ID=annotation2024;Name=OR1;Parent=BBa_R0051
BBa_J05030	.	engineered_region	3424	3472	.	+	0	ID=annotation7067;Name=BBa_R0051;Parent=BBa_R0051
BBa_J05030	.	non_covalent_binding_site	3424	3440	.	+	0	ID=annotation2025;Name=OR2;Parent=BBa_R0051
BBa_J05030	.	promoter	3438	3443	.	+	0	ID=annotation2023;Name=-35;Parent=BBa_R0051
BBa_J05030	.	promoter	3461	3466	.	+	0	ID=annotation2022;Name=-10;Parent=BBa_R0051
BBa_J05030	.	terminator	3287	3366	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_J05030	.	stem_loop	3298	3341	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_J05030	.	engineered_region	3287	3366	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_J05030	.	terminator	1130	1209	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_J05030	.	stem_loop	1141	1184	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_J05030	.	engineered_region	1130	1209	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_J05030	.	terminator	3150	3229	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_J05030	.	stem_loop	3161	3204	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_J05030	.	engineered_region	3150	3229	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_J05030	.	ribosome_entry_site	346	357	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_J05030	.	conserved_region	350	353	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_J05030	.	terminator	1	80	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_J05030	.	stem_loop	12	55	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_J05030	.	engineered_region	1	80	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_J05030	.	terminator	4345	4385	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_J05030	.	polyA_site	4372	4385	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_J05030	.	engineered_region	4345	4385	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_J05030	.	stem_loop	4352	4371	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_J05030	.	stop_codon	4378	4378	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_J05030	.	ribosome_entry_site	3511	3522	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_J05030	.	conserved_region	3515	3518	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_J05030	.	terminator	1355	1395	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_J05030	.	polyA_site	1382	1395	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_J05030	.	engineered_region	1355	1395	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_J05030	.	stem_loop	1362	1381	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_J05030	.	stop_codon	1388	1388	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_J05030	.	promoter	138	337	.	+	0	ID=BBa_R0010;Name=BBa_R0010
BBa_J05030	.	start_codon	310	310	.	+	0	ID=annotation1961227;Name=start;Parent=BBa_R0010
BBa_J05030	.	CDS	138	225	.	+	0	ID=annotation1961221;Name=end of LacI coding region (inactive);Parent=BBa_R0010
BBa_J05030	.	promoter	298	303	.	+	0	ID=annotation1961225;Name=-10;Parent=BBa_R0010
BBa_J05030	.	non_covalent_binding_site	303	337	.	+	0	ID=annotation1961226;Name=LacI binding site;Parent=BBa_R0010
BBa_J05030	.	non_covalent_binding_site	226	263	.	+	0	ID=annotation1961223;Name=CAP binding site;Parent=BBa_R0010
BBa_J05030	.	engineered_region	138	337	.	+	0	ID=annotation1961222;Name=BBa_R0010;Parent=BBa_R0010
BBa_J05030	.	promoter	274	279	.	+	0	ID=annotation1961224;Name=-35;Parent=BBa_R0010
BBa_J05030	.	terminator	2281	2360	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_J05030	.	stem_loop	2292	2335	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_J05030	.	engineered_region	2281	2360	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_J05030	.	terminator	3375	3415	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_J05030	.	polyA_site	3402	3415	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_J05030	.	engineered_region	3375	3415	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_J05030	.	stem_loop	3382	3401	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_J05030	.	stop_codon	3408	3408	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_J05030	.	ribosome_entry_site	398	409	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_J05030	.	conserved_region	402	405	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_J05030	.	sequence_feature	1491	1526	.	+	0	ID=BBa_J05021;Name=BBa_J05021
BBa_J05030	.	CDS	416	1096	.	+	0	ID=BBa_E1010;Name=BBa_E1010
BBa_J05030	.	CDS	416	1090	.	+	0	ID=annotation1014044;Name=mrfp1;Parent=BBa_E1010
BBa_J05030	.	engineered_tag	1097	1121	.	+	0	ID=annotation2214014;Name=Help:Barcodes;Parent=BBa_E1010
BBa_J05030	.	CDS	1553	2272	.	+	0	ID=BBa_E0040;Name=BBa_E0040
BBa_J05030	.	CDS	1553	2272	.	+	0	ID=annotation1934520;Name=GFP protein;Parent=BBa_E0040
BBa_J05030	.	terminator	1267	1346	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_J05030	.	stem_loop	1278	1321	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_J05030	.	engineered_region	1267	1346	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_J05030	.	terminator	2369	2409	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_J05030	.	polyA_site	2396	2409	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_J05030	.	engineered_region	2369	2409	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_J05030	.	stem_loop	2376	2395	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_J05030	.	stop_codon	2402	2402	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_J05030	.	terminator	3238	3278	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_J05030	.	polyA_site	3265	3278	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_J05030	.	engineered_region	3238	3278	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_J05030	.	stem_loop	3245	3264	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_J05030	.	stop_codon	3271	3271	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_J05030	.	sequence_feature	1461	1482	.	+	0	ID=BBa_J05020;Name=BBa_J05020
BBa_J05030	.	terminator	89	129	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_J05030	.	polyA_site	116	129	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_J05030	.	engineered_region	89	129	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_J05030	.	stem_loop	96	115	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_J05030	.	stop_codon	122	122	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_J05030	.	CDS	2436	3116	.	+	0	ID=BBa_E1010;Name=BBa_E1010
BBa_J05030	.	CDS	2436	3110	.	+	0	ID=annotation1014044;Name=mrfp1;Parent=BBa_E1010
BBa_J05030	.	engineered_tag	3117	3141	.	+	0	ID=annotation2214014;Name=Help:Barcodes;Parent=BBa_E1010
BBa_J05030	.	terminator	1218	1258	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_J05030	.	polyA_site	1245	1258	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_J05030	.	engineered_region	1218	1258	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_J05030	.	stem_loop	1225	1244	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_J05030	.	stop_codon	1251	1251	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_J05030	.	ribosome_entry_site	1535	1546	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_J05030	.	conserved_region	1539	1542	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_J05030	.	sequence_feature	3481	3502	.	+	0	ID=BBa_J05020;Name=BBa_J05020
BBa_J05030	.	CDS	3529	4248	.	+	0	ID=BBa_E0040;Name=BBa_E0040
BBa_J05030	.	CDS	3529	4248	.	+	0	ID=annotation1934520;Name=GFP protein;Parent=BBa_E0040
BBa_J05030	.	sequence_feature	366	389	.	+	0	ID=BBa_J05010;Name=BBa_J05010
BBa_J05030	.	ribosome_entry_site	2418	2429	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_J05030	.	conserved_region	2422	2425	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_J05030	.	promoter	1404	1452	.	+	0	ID=BBa_R0051;Name=BBa_R0051
BBa_J05030	.	non_covalent_binding_site	1428	1444	.	+	0	ID=annotation2024;Name=OR1;Parent=BBa_R0051
BBa_J05030	.	engineered_region	1404	1452	.	+	0	ID=annotation7067;Name=BBa_R0051;Parent=BBa_R0051
BBa_J05030	.	non_covalent_binding_site	1404	1420	.	+	0	ID=annotation2025;Name=OR2;Parent=BBa_R0051
BBa_J05030	.	promoter	1418	1423	.	+	0	ID=annotation2023;Name=-35;Parent=BBa_R0051
BBa_J05030	.	promoter	1441	1446	.	+	0	ID=annotation2022;Name=-10;Parent=BBa_R0051
BBa_J05030	.	terminator	4257	4336	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_J05030	.	stem_loop	4268	4311	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_J05030	.	engineered_region	4257	4336	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
>BBa_J05030
ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctct
ctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagcaatacgcaaaccgcctctcc
ccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaat
taatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtg
agcggataacaatttcacacatactagagaaagaggagaaatactagagggggggggggggggggggggggggtactag
agaaagaggagaaatactagatggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaagg
ttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactg
aaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacg
ttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaactt
cgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgt
ggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacc
cggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaa
aaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccac
aacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagt
gctagtgtagatcgctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatc
tgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactag
agccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgct
ctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagtaacaccgtgcgtgttgac
tattttacctctggcggtgataatggttgctactagagggggggggggggggggggggggtactagagggggggggggg
gggggggggggggggggggggggggtactagagaaagaggagaaatactagatgcgtaaaggagaagaacttttcactg
gagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtga
tgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcact
actttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgc
ccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttga
aggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattg
gaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaatta
gacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtcct
tttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtcctt
cttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagagccaggcatcaa
ataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtc
acactggctcaccttcgggtgggcctttctgcgtttatatactagagaaagaggagaaatactagatggcttcctccga
agacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggt
gaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgctt
gggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaa
actgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggac
tcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgc
agaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaat
gcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctg
ccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaac
gtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgctactagagccaggcatcaa
ataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtc
acactggctcaccttcgggtgggcctttctgcgtttatatactagagccaggcatcaaataaaacgaaaggctcagtcg
aaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtg
ggcctttctgcgtttatatactagagtaacaccgtgcgtgttgactattttacctctggcggtgataatggttgctact
agagggggggggggggggggggggggtactagagaaagaggagaaatactagatgcgtaaaggagaagaacttttcact
ggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtg
atgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcac
tactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatg
cccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttg
aaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaatt
ggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaatt
agacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtcc
ttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtcct
tcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagagccaggcatca
aataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagt
cacactggctcaccttcgggtgggcctttctgcgtttata