LOCUS BBa_J05040 3732 bp DNA linear 21-Dec-2024 DEFINITION 4-State Device ACCESSION BBa_J05040 VERSION BBa_J05040.1 FEATURES Location/Qualifiers misc_feature 9..25 /label=OR1 misc_feature 33..49 /label=OR2 promoter 48..53 /label=-35 misc_feature 59..65 /label=mutate to obliterate OR3 promoter 71..76 /label=-10 promoter 1..82 /label=BBa_I12007 promoter 91..131 /label=BBa_J05215 misc_feature 144..147 /label=conserved RBS 140..151 /label=BBa_B0034 CDS 179..262 /label=Finger 1 misc_feature 212..235 /label=ZF-GAC CDS 263..346 /label=Finger 2 misc_feature 296..319 /label=ZF-GGG CDS 347..430 /label=Finger 3 misc_feature 380..403 /label=ZF-GGA misc_feature 593..617 /label=Help:Barcodes CDS 158..592 /label=BBa_J05108 stem_loop 637..680 /label=stem_loop misc_feature 626..705 /label=BBa_B0010 terminator 626..705 /label=BBa_B0010 stem_loop 721..740 /label=T7 TE misc_feature 747..747 /label=stop polyA_site 741..754 /label=polya misc_feature 714..754 /label=BBa_B0012 terminator 714..754 /label=BBa_B0012 promoter 800..805 /label=-10 promoter 777..782 /label=-35 misc_feature 787..803 /label=OR1 misc_feature 763..779 /label=OR2 misc_feature 763..811 /label=BBa_R0051 promoter 763..811 /label=BBa_R0051 promoter 820..860 /label=BBa_J05217 misc_feature 873..876 /label=conserved RBS 869..880 /label=BBa_B0034 CDS 908..991 /label=Finger 1 misc_feature 941..964 /label=ZF-GGG CDS 992..1075 /label=Finger 2 misc_feature 1025..1048 /label=ZF-GGC CDS 1076..1159 /label=Finger 3 misc_feature 1109..1132 /label=ZF-GGA misc_feature 1328..1352 /label=Help:Barcodes CDS 887..1327 /label=BBa_J05110 stem_loop 1372..1415 /label=stem_loop misc_feature 1361..1440 /label=BBa_B0010 terminator 1361..1440 /label=BBa_B0010 stem_loop 1456..1475 /label=T7 TE misc_feature 1482..1482 /label=stop polyA_site 1476..1489 /label=polya misc_feature 1449..1489 /label=BBa_B0012 terminator 1449..1489 /label=BBa_B0012 misc_feature 1506..1522 /label=OR1 misc_feature 1530..1546 /label=OR2 promoter 1545..1550 /label=-35 misc_feature 1556..1562 /label=mutate to obliterate OR3 promoter 1568..1573 /label=-10 promoter 1498..1579 /label=BBa_I12007 promoter 1588..1628 /label=BBa_J05217 misc_feature 1641..1644 /label=conserved RBS 1637..1648 /label=BBa_B0034 CDS 1676..1759 /label=Finger 1 misc_feature 1709..1732 /label=ZF-GGG CDS 1760..1843 /label=Finger 2 misc_feature 1793..1816 /label=ZF-GGC CDS 1844..1927 /label=Finger 3 misc_feature 1877..1900 /label=ZF-GGA misc_feature 2096..2120 /label=Help:Barcodes CDS 1655..2095 /label=BBa_J05110 RBS 2129..2143 /label=RBS-1\Strong RBS 2136..2140 /label=RBS misc_feature 2129..2143 /label=BBa_B0030 RBS 2129..2143 /label=BBa_B0030 CDS 2150..2869 /label=GFP protein CDS 2150..2869 /label=BBa_E0040 stem_loop 2889..2932 /label=stem_loop misc_feature 2878..2957 /label=BBa_B0010 terminator 2878..2957 /label=BBa_B0010 stem_loop 2973..2992 /label=T7 TE misc_feature 2999..2999 /label=stop polyA_site 2993..3006 /label=polya misc_feature 2966..3006 /label=BBa_B0012 terminator 2966..3006 /label=BBa_B0012 promoter 3052..3057 /label=-10 promoter 3029..3034 /label=-35 misc_feature 3039..3055 /label=OR1 misc_feature 3015..3031 /label=OR2 misc_feature 3015..3063 /label=BBa_R0051 promoter 3015..3063 /label=BBa_R0051 promoter 3072..3112 /label=BBa_J05218 misc_feature 3125..3128 /label=conserved RBS 3121..3132 /label=BBa_B0034 CDS 3160..3243 /label=Finger 1 misc_feature 3193..3216 /label=ZF-GGA CDS 3244..3327 /label=Finger 2 misc_feature 3277..3300 /label=ZF-GCC CDS 3328..3411 /label=Finger 3 misc_feature 3361..3384 /label=ZF-GGG misc_feature 3571..3595 /label=Help:Barcodes CDS 3139..3570 /label=BBa_J05111 stem_loop 3615..3658 /label=stem_loop misc_feature 3604..3683 /label=BBa_B0010 terminator 3604..3683 /label=BBa_B0010 stem_loop 3699..3718 /label=T7 TE misc_feature 3725..3725 /label=stop polyA_site 3719..3732 /label=polya misc_feature 3692..3732 /label=BBa_B0012 terminator 3692..3732 /label=BBa_B0012 ORIGIN 1 gcaaccatta tcaccgccag aggtaaaata gtcaacacgc acggtgttag atatttataa 61 atagtggtga tagatttaac gttactagag cctcgccccc gggggcgagg gccccgcctc 121 cggaggcggg gtactagaga aagaggagaa atactagatg ctggaaccgg gcgaaaaacc 181 gtataaatgc ccggaatgcg gcaaaagctt tagcgatccg ggcaacctgg tgcgccatca 241 gcgcacccat accggcgaaa aaccgtataa atgcccggaa tgcggcaaaa gctttagccg 301 cagcgataaa ctggtgcgcc atcagcgcac ccataccggc gaaaaaccgt ataaatgccc 361 ggaatgcggc aaaagcttta gccagcgcgc gcatctggaa cgccatcagc gcacccatac 421 cggcaaaaaa accagcgaat atattgatgg cctggaaacc cgcatgagcg cgtgcaccgc 481 gcagaaccag gaactgcaac gcaaagtgct gcatctggaa aaacagaacc tgagcctgct 541 ggaacagctg aaaaaactgc aagcgattgt ggtgcagagc accagctaat aacgctgata 601 gtgctagtgt agatcgctac tagagccagg catcaaataa aacgaaaggc tcagtcgaaa 661 gactgggcct ttcgttttat ctgttgtttg tcggtgaacg ctctctacta gagtcacact 721 ggctcacctt cgggtgggcc tttctgcgtt tatatactag agtaacaccg tgcgtgttga 781 ctattttacc tctggcggtg ataatggttg ctactagagc ctcgcccccg ggggcgaggg 841 ctccggcccc ggggccggag tactagagaa agaggagaaa tactagatgc tggaaccggg 901 cgaaaaaccg tataaatgcc cggaatgcgg caaaagcttt agccgcagcg ataaactggt 961 gcgccatcag cgcacccata ccggcgaaaa accgtataaa tgcccggaat gcggcaaaag 1021 ctttagcgat ccgggccatc tggtgcgcca tcagcgcacc cataccggcg aaaaaccgta 1081 taaatgcccg gaatgcggca aaagctttag ccagcgcgcg catctggaac gccatcagcg 1141 cacccatacc ggcaaaaaaa ccagcacccg cattcaggtg ctggaagaaa aagtggaaat 1201 gctgcataac ctggtggatg attggcagcg caaatataaa ctgctggaaa gcgaatttag 1261 cgataccaaa gaaaacctgc aaaaaagcat tgcgctgaac aacgaactgc aaaaagcgct 1321 gtaataactc tgatagtgct agtgtagatc tctactagag ccaggcatca aataaaacga 1381 aaggctcagt cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc 1441 tactagagtc acactggctc accttcgggt gggcctttct gcgtttatat actagaggca 1501 accattatca ccgccagagg taaaatagtc aacacgcacg gtgttagata tttataaata 1561 gtggtgatag atttaacgtt actagagcct cgcccccggg ggcgagggct ccggccccgg 1621 ggccggagta ctagagaaag aggagaaata ctagatgctg gaaccgggcg aaaaaccgta 1681 taaatgcccg gaatgcggca aaagctttag ccgcagcgat aaactggtgc gccatcagcg 1741 cacccatacc ggcgaaaaac cgtataaatg cccggaatgc ggcaaaagct ttagcgatcc 1801 gggccatctg gtgcgccatc agcgcaccca taccggcgaa aaaccgtata aatgcccgga 1861 atgcggcaaa agctttagcc agcgcgcgca tctggaacgc catcagcgca cccataccgg 1921 caaaaaaacc agcacccgca ttcaggtgct ggaagaaaaa gtggaaatgc tgcataacct 1981 ggtggatgat tggcagcgca aatataaact gctggaaagc gaatttagcg ataccaaaga 2041 aaacctgcaa aaaagcattg cgctgaacaa cgaactgcaa aaagcgctgt aataactctg 2101 atagtgctag tgtagatctc tactagagat taaagaggag aaatactaga tgcgtaaagg 2161 agaagaactt ttcactggag ttgtcccaat tcttgttgaa ttagatggtg atgttaatgg 2221 gcacaaattt tctgtcagtg gagagggtga aggtgatgca acatacggaa aacttaccct 2281 taaatttatt tgcactactg gaaaactacc tgttccatgg ccaacacttg tcactacttt 2341 cggttatggt gttcaatgct ttgcgagata cccagatcat atgaaacagc atgacttttt 2401 caagagtgcc atgcccgaag gttatgtaca ggaaagaact atatttttca aagatgacgg 2461 gaactacaag acacgtgctg aagtcaagtt tgaaggtgat acccttgtta atagaatcga 2521 gttaaaaggt attgatttta aagaagatgg aaacattctt ggacacaaat tggaatacaa 2581 ctataactca cacaatgtat acatcatggc agacaaacaa aagaatggaa tcaaagttaa 2641 cttcaaaatt agacacaaca ttgaagatgg aagcgttcaa ctagcagacc attatcaaca 2701 aaatactcca attggcgatg gccctgtcct tttaccagac aaccattacc tgtccacaca 2761 atctgccctt tcgaaagatc ccaacgaaaa gagagaccac atggtccttc ttgagtttgt 2821 aacagctgct gggattacac atggcatgga tgaactatac aaataataat actagagcca 2881 ggcatcaaat aaaacgaaag gctcagtcga aagactgggc ctttcgtttt atctgttgtt 2941 tgtcggtgaa cgctctctac tagagtcaca ctggctcacc ttcgggtggg cctttctgcg 3001 tttatatact agagtaacac cgtgcgtgtt gactatttta cctctggcgg tgataatggt 3061 tgctactaga gcgtcccctc cggaggggac ggccccgcct ccggaggcgg ggtactagag 3121 aaagaggaga aatactagat gctggaaccg ggcgaaaaac cgtataaatg cccggaatgc 3181 ggcaaaagct ttagccagcg cgcgcatctg gaacgccatc agcgcaccca taccggcgaa 3241 aaaccgtata aatgcccgga atgcggcaaa agctttagcg attgccgcga tctggcgcgc 3301 catcagcgca cccataccgg cgaaaaaccg tataaatgcc cggaatgcgg caaaagcttt 3361 agccgcagcg ataaactggt gcgccatcag cgcacccata ccggcaaaaa aaccagccag 3421 cagcgccatg tgctggaaag cgaaaaaaac cagctgctgc aacaggtgga tcatctgaaa 3481 caggaaatta gccgcctggt gcgcgaacgc gatgcgtata aagaaaaata tgaaaaactg 3541 gtgagcagcg gctttcgcga aaactaataa cactgatagt gctagtgtag atcactacta 3601 gagccaggca tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt cgttttatct 3661 gttgtttgtc ggtgaacgct ctctactaga gtcacactgg ctcaccttcg ggtgggcctt 3721 tctgcgttta ta //