LOCUS BBa_J05050 6111 bp DNA linear 21-Dec-2024 DEFINITION Counter ACCESSION BBa_J05050 VERSION BBa_J05050.1 FEATURES Location/Qualifiers misc_feature 1..19 /label=TetR 1 misc_feature 1..54 /label=BBa_R0040 promoter 20..25 /label=-35 misc_feature 26..44 /label=TetR 2 promoter 43..48 /label=-10 promoter 1..54 /label=BBa_R0040 misc_feature 67..70 /label=conserved RBS 63..74 /label=BBa_B0034 misc_feature 1170..1208 /label=LVA CDS 81..1208 /label=lacI-LVA misc_feature 81..1208 /label=BBa_C0012 misc_feature 1209..1233 /label=Help:Barcodes CDS 81..1208 /label=BBa_C0012 stem_loop 1253..1296 /label=stem_loop misc_feature 1242..1321 /label=BBa_B0010 terminator 1242..1321 /label=BBa_B0010 stem_loop 1337..1356 /label=T7 TE misc_feature 1363..1363 /label=stop polyA_site 1357..1370 /label=polya misc_feature 1330..1370 /label=BBa_B0012 terminator 1330..1370 /label=BBa_B0012 misc_feature 1381..1397 /label=lac O1 promoter 1398..1403 /label=-35 misc_feature 1404..1420 /label=lac O1 promoter 1421..1426 /label=-10 misc_feature 1379..1432 /label=BBa_R0011 promoter 1379..1432 /label=BBa_R0011 misc_feature 1446..1449 /label=conserved RBS 1442..1453 /label=BBa_B0034 CDS 1463..2170 /label=cI lambda misc_feature 2171..2203 /label=LVA misc_feature 2210..2234 /label=Help:Barcodes CDS 1460..2209 /label=BBa_C0051 stem_loop 2254..2297 /label=stem_loop misc_feature 2243..2322 /label=BBa_B0010 terminator 2243..2322 /label=BBa_B0010 stem_loop 2338..2357 /label=T7 TE misc_feature 2364..2364 /label=stop polyA_site 2358..2371 /label=polya misc_feature 2331..2371 /label=BBa_B0012 terminator 2331..2371 /label=BBa_B0012 promoter 1..2371 /label=BBa_J05500 misc_feature 2388..2404 /label=OR1 misc_feature 2412..2428 /label=OR2 promoter 2427..2432 /label=-35 misc_feature 2438..2444 /label=mutate to obliterate OR3 promoter 2450..2455 /label=-10 promoter 2380..2461 /label=BBa_I12007 promoter 2470..2510 /label=BBa_J05215 misc_feature 2523..2526 /label=conserved RBS 2519..2530 /label=BBa_B0034 CDS 2558..2641 /label=Finger 1 misc_feature 2591..2614 /label=ZF-GAC CDS 2642..2725 /label=Finger 2 misc_feature 2675..2698 /label=ZF-GGG CDS 2726..2809 /label=Finger 3 misc_feature 2759..2782 /label=ZF-GGA misc_feature 2972..2996 /label=Help:Barcodes CDS 2537..2971 /label=BBa_J05108 stem_loop 3016..3059 /label=stem_loop misc_feature 3005..3084 /label=BBa_B0010 terminator 3005..3084 /label=BBa_B0010 stem_loop 3100..3119 /label=T7 TE misc_feature 3126..3126 /label=stop polyA_site 3120..3133 /label=polya misc_feature 3093..3133 /label=BBa_B0012 terminator 3093..3133 /label=BBa_B0012 promoter 3179..3184 /label=-10 promoter 3156..3161 /label=-35 misc_feature 3166..3182 /label=OR1 misc_feature 3142..3158 /label=OR2 misc_feature 3142..3190 /label=BBa_R0051 promoter 3142..3190 /label=BBa_R0051 promoter 3199..3239 /label=BBa_J05217 misc_feature 3252..3255 /label=conserved RBS 3248..3259 /label=BBa_B0034 CDS 3287..3370 /label=Finger 1 misc_feature 3320..3343 /label=ZF-GGG CDS 3371..3454 /label=Finger 2 misc_feature 3404..3427 /label=ZF-GGC CDS 3455..3538 /label=Finger 3 misc_feature 3488..3511 /label=ZF-GGA misc_feature 3707..3731 /label=Help:Barcodes CDS 3266..3706 /label=BBa_J05110 stem_loop 3751..3794 /label=stem_loop misc_feature 3740..3819 /label=BBa_B0010 terminator 3740..3819 /label=BBa_B0010 stem_loop 3835..3854 /label=T7 TE misc_feature 3861..3861 /label=stop polyA_site 3855..3868 /label=polya misc_feature 3828..3868 /label=BBa_B0012 terminator 3828..3868 /label=BBa_B0012 misc_feature 3885..3901 /label=OR1 misc_feature 3909..3925 /label=OR2 promoter 3924..3929 /label=-35 misc_feature 3935..3941 /label=mutate to obliterate OR3 promoter 3947..3952 /label=-10 promoter 3877..3958 /label=BBa_I12007 promoter 3967..4007 /label=BBa_J05217 misc_feature 4020..4023 /label=conserved RBS 4016..4027 /label=BBa_B0034 CDS 4055..4138 /label=Finger 1 misc_feature 4088..4111 /label=ZF-GGG CDS 4139..4222 /label=Finger 2 misc_feature 4172..4195 /label=ZF-GGC CDS 4223..4306 /label=Finger 3 misc_feature 4256..4279 /label=ZF-GGA misc_feature 4475..4499 /label=Help:Barcodes CDS 4034..4474 /label=BBa_J05110 RBS 4508..4522 /label=RBS-1\Strong RBS 4515..4519 /label=RBS misc_feature 4508..4522 /label=BBa_B0030 RBS 4508..4522 /label=BBa_B0030 CDS 4529..5248 /label=GFP protein CDS 4529..5248 /label=BBa_E0040 stem_loop 5268..5311 /label=stem_loop misc_feature 5257..5336 /label=BBa_B0010 terminator 5257..5336 /label=BBa_B0010 stem_loop 5352..5371 /label=T7 TE misc_feature 5378..5378 /label=stop polyA_site 5372..5385 /label=polya misc_feature 5345..5385 /label=BBa_B0012 terminator 5345..5385 /label=BBa_B0012 promoter 5431..5436 /label=-10 promoter 5408..5413 /label=-35 misc_feature 5418..5434 /label=OR1 misc_feature 5394..5410 /label=OR2 misc_feature 5394..5442 /label=BBa_R0051 promoter 5394..5442 /label=BBa_R0051 promoter 5451..5491 /label=BBa_J05218 misc_feature 5504..5507 /label=conserved RBS 5500..5511 /label=BBa_B0034 CDS 5539..5622 /label=Finger 1 misc_feature 5572..5595 /label=ZF-GGA CDS 5623..5706 /label=Finger 2 misc_feature 5656..5679 /label=ZF-GCC CDS 5707..5790 /label=Finger 3 misc_feature 5740..5763 /label=ZF-GGG misc_feature 5950..5974 /label=Help:Barcodes CDS 5518..5949 /label=BBa_J05111 stem_loop 5994..6037 /label=stem_loop misc_feature 5983..6062 /label=BBa_B0010 terminator 5983..6062 /label=BBa_B0010 stem_loop 6078..6097 /label=T7 TE misc_feature 6104..6104 /label=stop polyA_site 6098..6111 /label=polya misc_feature 6071..6111 /label=BBa_B0012 terminator 6071..6111 /label=BBa_B0012 misc_feature 2380..6111 /label=BBa_J05040 ORIGIN 1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag 61 agaaagagga gaaatactag atggtgaatg tgaaaccagt aacgttatac gatgtcgcag 121 agtatgccgg tgtctcttat cagaccgttt cccgcgtggt gaaccaggcc agccacgttt 181 ctgcgaaaac gcgggaaaaa gtggaagcgg cgatggcgga gctgaattac attcccaacc 241 gcgtggcaca acaactggcg ggcaaacagt cgttgctgat tggcgttgcc acctccagtc 301 tggccctgca cgcgccgtcg caaattgtcg cggcgattaa atctcgcgcc gatcaactgg 361 gtgccagcgt ggtggtgtcg atggtagaac gaagcggcgt cgaagcctgt aaagcggcgg 421 tgcacaatct tctcgcgcaa cgcgtcagtg ggctgatcat taactatccg ctggatgacc 481 aggatgccat tgctgtggaa gctgcctgca ctaatgttcc ggcgttattt cttgatgtct 541 ctgaccagac acccatcaac agtattattt tctcccatga agacggtacg cgactgggcg 601 tggagcatct ggtcgcattg ggtcaccagc aaatcgcgct gttagcgggc ccattaagtt 661 ctgtctcggc gcgtctgcgt ctggctggct ggcataaata tctcactcgc aatcaaattc 721 agccgatagc ggaacgggaa ggcgactgga gtgccatgtc cggttttcaa caaaccatgc 781 aaatgctgaa tgagggcatc gttcccactg cgatgctggt tgccaacgat cagatggcgc 841 tgggcgcaat gcgcgccatt accgagtccg ggctgcgcgt tggtgcggat atctcggtag 901 tgggatacga cgataccgaa gacagctcat gttatatccc gccgttaacc accatcaaac 961 aggattttcg cctgctgggg caaaccagcg tggaccgctt gctgcaactc tctcagggcc 1021 aggcggtgaa gggcaatcag ctgttgcccg tctcactggt gaaaagaaaa accaccctgg 1081 cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac 1141 gacaggtttc ccgactggaa agcgggcagg ctgcaaacga cgaaaactac gctttagtag 1201 cttaataact ctgatagtgc tagtgtagat ctctactaga gccaggcatc aaataaaacg 1261 aaaggctcag tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct 1321 ctactagagt cacactggct caccttcggg tgggcctttc tgcgtttata tactagagaa 1381 ttgtgagcgg ataacaattg acattgtgag cggataacaa gatactgagc acatactaga 1441 gaaagaggag aaatactaga tgagcacaaa aaagaaacca ttaacacaag agcagcttga 1501 ggacgcacgt cgccttaaag caatttatga aaaaaagaaa aatgaacttg gcttatccca 1561 ggaatctgtc gcagacaaga tggggatggg gcagtcaggc gttggtgctt tatttaatgg 1621 catcaatgca ttaaatgctt ataacgccgc attgcttgca aaaattctca aagttagcgt 1681 tgaagaattt agcccttcaa tcgccagaga aatctacgag atgtatgaag cggttagtat 1741 gcagccgtca cttagaagtg agtatgagta ccctgttttt tctcatgttc aggcagggat 1801 gttctcacct gagcttagaa cctttaccaa aggtgatgcg gagagatggg taagcacaac 1861 caaaaaagcc agtgattctg cattctggct tgaggttgaa ggtaattcca tgaccgcacc 1921 aacaggctcc aagccaagct ttcctgacgg aatgttaatt ctcgttgacc ctgagcaggc 1981 tgttgagcca ggtgatttct gcatagccag acttgggggt gatgagttta ccttcaagaa 2041 actgatcagg gatagcggtc aggtgttttt acaaccacta aacccacagt acccaatgat 2101 cccatgcaat gagagttgtt ccgttgtggg gaaagttatc gctagtcagt ggcctgaaga 2161 gacgtttggc gctgcaaacg acgaaaacta cgctttagta gcttaataac gctgatagtg 2221 ctagtgtaga tcgctactag agccaggcat caaataaaac gaaaggctca gtcgaaagac 2281 tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc tctactagag tcacactggc 2341 tcaccttcgg gtgggccttt ctgcgtttat atactagagg caaccattat caccgccaga 2401 ggtaaaatag tcaacacgca cggtgttaga tatttataaa tagtggtgat agatttaacg 2461 ttactagagc ctcgcccccg ggggcgaggg ccccgcctcc ggaggcgggg tactagagaa 2521 agaggagaaa tactagatgc tggaaccggg cgaaaaaccg tataaatgcc cggaatgcgg 2581 caaaagcttt agcgatccgg gcaacctggt gcgccatcag cgcacccata ccggcgaaaa 2641 accgtataaa tgcccggaat gcggcaaaag ctttagccgc agcgataaac tggtgcgcca 2701 tcagcgcacc cataccggcg aaaaaccgta taaatgcccg gaatgcggca aaagctttag 2761 ccagcgcgcg catctggaac gccatcagcg cacccatacc ggcaaaaaaa ccagcgaata 2821 tattgatggc ctggaaaccc gcatgagcgc gtgcaccgcg cagaaccagg aactgcaacg 2881 caaagtgctg catctggaaa aacagaacct gagcctgctg gaacagctga aaaaactgca 2941 agcgattgtg gtgcagagca ccagctaata acgctgatag tgctagtgta gatcgctact 3001 agagccaggc atcaaataaa acgaaaggct cagtcgaaag actgggcctt tcgttttatc 3061 tgttgtttgt cggtgaacgc tctctactag agtcacactg gctcaccttc gggtgggcct 3121 ttctgcgttt atatactaga gtaacaccgt gcgtgttgac tattttacct ctggcggtga 3181 taatggttgc tactagagcc tcgcccccgg gggcgagggc tccggccccg gggccggagt 3241 actagagaaa gaggagaaat actagatgct ggaaccgggc gaaaaaccgt ataaatgccc 3301 ggaatgcggc aaaagcttta gccgcagcga taaactggtg cgccatcagc gcacccatac 3361 cggcgaaaaa ccgtataaat gcccggaatg cggcaaaagc tttagcgatc cgggccatct 3421 ggtgcgccat cagcgcaccc ataccggcga aaaaccgtat aaatgcccgg aatgcggcaa 3481 aagctttagc cagcgcgcgc atctggaacg ccatcagcgc acccataccg gcaaaaaaac 3541 cagcacccgc attcaggtgc tggaagaaaa agtggaaatg ctgcataacc tggtggatga 3601 ttggcagcgc aaatataaac tgctggaaag cgaatttagc gataccaaag aaaacctgca 3661 aaaaagcatt gcgctgaaca acgaactgca aaaagcgctg taataactct gatagtgcta 3721 gtgtagatct ctactagagc caggcatcaa ataaaacgaa aggctcagtc gaaagactgg 3781 gcctttcgtt ttatctgttg tttgtcggtg aacgctctct actagagtca cactggctca 3841 ccttcgggtg ggcctttctg cgtttatata ctagaggcaa ccattatcac cgccagaggt 3901 aaaatagtca acacgcacgg tgttagatat ttataaatag tggtgataga tttaacgtta 3961 ctagagcctc gcccccgggg gcgagggctc cggccccggg gccggagtac tagagaaaga 4021 ggagaaatac tagatgctgg aaccgggcga aaaaccgtat aaatgcccgg aatgcggcaa 4081 aagctttagc cgcagcgata aactggtgcg ccatcagcgc acccataccg gcgaaaaacc 4141 gtataaatgc ccggaatgcg gcaaaagctt tagcgatccg ggccatctgg tgcgccatca 4201 gcgcacccat accggcgaaa aaccgtataa atgcccggaa tgcggcaaaa gctttagcca 4261 gcgcgcgcat ctggaacgcc atcagcgcac ccataccggc aaaaaaacca gcacccgcat 4321 tcaggtgctg gaagaaaaag tggaaatgct gcataacctg gtggatgatt ggcagcgcaa 4381 atataaactg ctggaaagcg aatttagcga taccaaagaa aacctgcaaa aaagcattgc 4441 gctgaacaac gaactgcaaa aagcgctgta ataactctga tagtgctagt gtagatctct 4501 actagagatt aaagaggaga aatactagat gcgtaaagga gaagaacttt tcactggagt 4561 tgtcccaatt cttgttgaat tagatggtga tgttaatggg cacaaatttt ctgtcagtgg 4621 agagggtgaa ggtgatgcaa catacggaaa acttaccctt aaatttattt gcactactgg 4681 aaaactacct gttccatggc caacacttgt cactactttc ggttatggtg ttcaatgctt 4741 tgcgagatac ccagatcata tgaaacagca tgactttttc aagagtgcca tgcccgaagg 4801 ttatgtacag gaaagaacta tatttttcaa agatgacggg aactacaaga cacgtgctga 4861 agtcaagttt gaaggtgata cccttgttaa tagaatcgag ttaaaaggta ttgattttaa 4921 agaagatgga aacattcttg gacacaaatt ggaatacaac tataactcac acaatgtata 4981 catcatggca gacaaacaaa agaatggaat caaagttaac ttcaaaatta gacacaacat 5041 tgaagatgga agcgttcaac tagcagacca ttatcaacaa aatactccaa ttggcgatgg 5101 ccctgtcctt ttaccagaca accattacct gtccacacaa tctgcccttt cgaaagatcc 5161 caacgaaaag agagaccaca tggtccttct tgagtttgta acagctgctg ggattacaca 5221 tggcatggat gaactataca aataataata ctagagccag gcatcaaata aaacgaaagg 5281 ctcagtcgaa agactgggcc tttcgtttta tctgttgttt gtcggtgaac gctctctact 5341 agagtcacac tggctcacct tcgggtgggc ctttctgcgt ttatatacta gagtaacacc 5401 gtgcgtgttg actattttac ctctggcggt gataatggtt gctactagag cgtcccctcc 5461 ggaggggacg gccccgcctc cggaggcggg gtactagaga aagaggagaa atactagatg 5521 ctggaaccgg gcgaaaaacc gtataaatgc ccggaatgcg gcaaaagctt tagccagcgc 5581 gcgcatctgg aacgccatca gcgcacccat accggcgaaa aaccgtataa atgcccggaa 5641 tgcggcaaaa gctttagcga ttgccgcgat ctggcgcgcc atcagcgcac ccataccggc 5701 gaaaaaccgt ataaatgccc ggaatgcggc aaaagcttta gccgcagcga taaactggtg 5761 cgccatcagc gcacccatac cggcaaaaaa accagccagc agcgccatgt gctggaaagc 5821 gaaaaaaacc agctgctgca acaggtggat catctgaaac aggaaattag ccgcctggtg 5881 cgcgaacgcg atgcgtataa agaaaaatat gaaaaactgg tgagcagcgg ctttcgcgaa 5941 aactaataac actgatagtg ctagtgtaga tcactactag agccaggcat caaataaaac 6001 gaaaggctca gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc 6061 tctactagag tcacactggc tcaccttcgg gtgggccttt ctgcgtttat a //