Counter
type: ComponentDefinition
persistent identity: BBa J05050
display id: BBa_J05050
version: 1
was generated by: iGEM to SBOL conversion
description: Counter
created: 2005-09-14T11:00:00Z
modified: 2015-08-31T04:08:15Z
owned by: james
owned by: myers
mutable description: This is the design of the counter.
discontinued: false
dominant: false
group u list: _34_
m user id: 0
owner id: 412
owning group id: 34
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: ETH Zurich iGEM Team 2005
type: DnaRegion
role: SO:0000804
role: partType/Project
component: BBa_J05500
component: BBa_J05040
sequence annotation: BBa_J05500
sequence annotation: BBa_J05040
sequence: https://synbiohub.org/public/igem/BBa_J05050_sequence/1

BBa_J05500
type: Component
persistent identity: component2240998
display id: component2240998
version: 1
top level: Counter
definition: EPD
access: public

BBa_J05040
type: Component
persistent identity: component2241069
display id: component2241069
version: 1
top level: Counter
definition: 4-SD
access: public

BBa_J05500
type: SequenceAnnotation
persistent identity: annotation2240998
display id: annotation2240998
version: 1
top level: Counter
location: https://synbiohub.org/public/igem/BBa_J05050/annotation2240998/range2240998/1
component: BBa_J05500

https://synbiohub.org/public/igem/BBa_J05050/annotation2240998/range2240998/1
type: Range
persistent identity: range2240998
display id: range2240998
version: 1
top level: Counter
direction: direction/reverse
start: 1
end: 2371
orientation: inline

BBa_J05040
type: SequenceAnnotation
persistent identity: annotation2241069
display id: annotation2241069
version: 1
top level: Counter
location: https://synbiohub.org/public/igem/BBa_J05050/annotation2241069/range2241069/1
component: BBa_J05040

https://synbiohub.org/public/igem/BBa_J05050/annotation2241069/range2241069/1
type: Range
persistent identity: range2241069
display id: range2241069
version: 1
top level: Counter
direction: direction/reverse
start: 2380
end: 6111
orientation: inline

4-SD
type: ComponentDefinition
persistent identity: BBa J05040
display id: BBa_J05040
version: 1
was generated by: iGEM to SBOL conversion
description: 4-State Device
created: 2005-09-20T11:00:00Z
modified: 2015-08-31T04:08:15Z
owned by: james
owned by: myers
mutable description: The 4-State Device is a toggle switch that sequenctially progresses throuh 4 states with alternating inputs from Pr and Prm. It can be used to function in a counter. The interface promotors from the EPD are included.
discontinued: false
dominant: false
group u list: _34_
m user id: 0
owner id: 412
owning group id: 34
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: NOR group
type: DnaRegion
role: partType/Device
role: SO:0000804
component: BBa_B0012
component: BBa_B0034
component: BBa_J05217
component: BBa_B0030
component: BBa_J05110
component: BBa_J05215
component: BBa_B0010
component: BBa_J05108
component: BBa_R0051
component: BBa_I12007
component: BBa_E0040
component: BBa_J05218
component: BBa_J05111
sequence annotation: BBa_I12007
sequence annotation: BBa_J05215
sequence annotation: BBa_B0034
sequence annotation: BBa_J05108
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence annotation: BBa_R0051
sequence annotation: BBa_J05217
sequence annotation: BBa_J05110
sequence annotation: BBa_B0030
sequence annotation: BBa_E0040
sequence annotation: BBa_J05218
sequence annotation: BBa_J05111
sequence: https://synbiohub.org/public/igem/BBa_J05040_sequence/1

BBa_B0012
type: Component
persistent identity: component1706793
display id: component1706793
version: 1
top level: 4-SD
definition: BBa_B0012
access: public

BBa_B0034
type: Component
persistent identity: component1706820
display id: component1706820
version: 1
top level: 4-SD
definition: BBa_B0034
access: public

BBa_J05217
type: Component
persistent identity: component1706815
display id: component1706815
version: 1
top level: 4-SD
definition: BBa_J05217
access: public

BBa_B0030
type: Component
persistent identity: component1706833
display id: component1706833
version: 1
top level: 4-SD
definition: BBa_B0030
access: public

BBa_J05110
type: Component
persistent identity: component1706824
display id: component1706824
version: 1
top level: 4-SD
definition: R2
access: public

BBa_B0012
type: Component
persistent identity: component1706896
display id: component1706896
version: 1
top level: 4-SD
definition: BBa_B0012
access: public

BBa_B0034
type: Component
persistent identity: component1706728
display id: component1706728
version: 1
top level: 4-SD
definition: BBa_B0034
access: public

BBa_J05215
type: Component
persistent identity: component1706723
display id: component1706723
version: 1
top level: 4-SD
definition: BBa_J05215
access: public

BBa_B0010
type: Component
persistent identity: component1706738
display id: component1706738
version: 1
top level: 4-SD
definition: BBa_B0010
access: public

BBa_J05108
type: Component
persistent identity: component1706732
display id: component1706732
version: 1
top level: 4-SD
definition: R1
access: public

BBa_R0051
type: Component
persistent identity: component1706763
display id: component1706763
version: 1
top level: 4-SD
definition: cI lam
access: public

BBa_B0012
type: Component
persistent identity: component1706748
display id: component1706748
version: 1
top level: 4-SD
definition: BBa_B0012
access: public

BBa_B0034
type: Component
persistent identity: component1706773
display id: component1706773
version: 1
top level: 4-SD
definition: BBa_B0034
access: public

BBa_J05217
type: Component
persistent identity: component1706768
display id: component1706768
version: 1
top level: 4-SD
definition: BBa_J05217
access: public

BBa_I12007
type: Component
persistent identity: component1706721
display id: component1706721
version: 1
top level: 4-SD
definition: Prm +
access: public

BBa_B0010
type: Component
persistent identity: component1706841
display id: component1706841
version: 1
top level: 4-SD
definition: BBa_B0010
access: public

BBa_E0040
type: Component
persistent identity: component1706836
display id: component1706836
version: 1
top level: 4-SD
definition: GFP
access: public

BBa_R0051
type: Component
persistent identity: component1706866
display id: component1706866
version: 1
top level: 4-SD
definition: cI lam
access: public

BBa_B0012
type: Component
persistent identity: component1706851
display id: component1706851
version: 1
top level: 4-SD
definition: BBa_B0012
access: public

BBa_B0034
type: Component
persistent identity: component1706876
display id: component1706876
version: 1
top level: 4-SD
definition: BBa_B0034
access: public

BBa_J05218
type: Component
persistent identity: component1706871
display id: component1706871
version: 1
top level: 4-SD
definition: BBa_J05218
access: public

BBa_B0010
type: Component
persistent identity: component1706886
display id: component1706886
version: 1
top level: 4-SD
definition: BBa_B0010
access: public

BBa_J05111
type: Component
persistent identity: component1706880
display id: component1706880
version: 1
top level: 4-SD
definition: R4
access: public

BBa_B0010
type: Component
persistent identity: component1706783
display id: component1706783
version: 1
top level: 4-SD
definition: BBa_B0010
access: public

BBa_J05110
type: Component
persistent identity: component1706777
display id: component1706777
version: 1
top level: 4-SD
definition: R2
access: public

BBa_I12007
type: Component
persistent identity: component1706813
display id: component1706813
version: 1
top level: 4-SD
definition: Prm +
access: public

BBa_I12007
type: SequenceAnnotation
persistent identity: annotation1706721
display id: annotation1706721
version: 1
top level: 4-SD
location: https://synbiohub.org/public/igem/BBa_J05040/annotation1706721/range1706721/1
component: BBa_I12007

https://synbiohub.org/public/igem/BBa_J05040/annotation1706721/range1706721/1
type: Range
persistent identity: range1706721
display id: range1706721
version: 1
top level: 4-SD
direction: direction/forward
start: 1
end: 82
orientation: inline

BBa_J05215
type: SequenceAnnotation
persistent identity: annotation1706723
display id: annotation1706723
version: 1
top level: 4-SD
location: https://synbiohub.org/public/igem/BBa_J05040/annotation1706723/range1706723/1
component: BBa_J05215

https://synbiohub.org/public/igem/BBa_J05040/annotation1706723/range1706723/1
type: Range
persistent identity: range1706723
display id: range1706723
version: 1
top level: 4-SD
direction: direction/forward
start: 91
end: 131
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1706728
display id: annotation1706728
version: 1
top level: 4-SD
location: https://synbiohub.org/public/igem/BBa_J05040/annotation1706728/range1706728/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_J05040/annotation1706728/range1706728/1
type: Range
persistent identity: range1706728
display id: range1706728
version: 1
top level: 4-SD
direction: direction/forward
start: 140
end: 151
orientation: inline

BBa_J05108
type: SequenceAnnotation
persistent identity: annotation1706732
display id: annotation1706732
version: 1
top level: 4-SD
location: https://synbiohub.org/public/igem/BBa_J05040/annotation1706732/range1706732/1
component: BBa_J05108

https://synbiohub.org/public/igem/BBa_J05040/annotation1706732/range1706732/1
type: Range
persistent identity: range1706732
display id: range1706732
version: 1
top level: 4-SD
direction: direction/forward
start: 158
end: 592
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1706738
display id: annotation1706738
version: 1
top level: 4-SD
location: https://synbiohub.org/public/igem/BBa_J05040/annotation1706738/range1706738/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_J05040/annotation1706738/range1706738/1
type: Range
persistent identity: range1706738
display id: range1706738
version: 1
top level: 4-SD
direction: direction/forward
start: 626
end: 705
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1706748
display id: annotation1706748
version: 1
top level: 4-SD
location: https://synbiohub.org/public/igem/BBa_J05040/annotation1706748/range1706748/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_J05040/annotation1706748/range1706748/1
type: Range
persistent identity: range1706748
display id: range1706748
version: 1
top level: 4-SD
direction: direction/forward
start: 714
end: 754
orientation: inline

BBa_R0051
type: SequenceAnnotation
persistent identity: annotation1706763
display id: annotation1706763
version: 1
top level: 4-SD
location: https://synbiohub.org/public/igem/BBa_J05040/annotation1706763/range1706763/1
component: BBa_R0051

https://synbiohub.org/public/igem/BBa_J05040/annotation1706763/range1706763/1
type: Range
persistent identity: range1706763
display id: range1706763
version: 1
top level: 4-SD
direction: direction/forward
start: 763
end: 811
orientation: inline

BBa_J05217
type: SequenceAnnotation
persistent identity: annotation1706768
display id: annotation1706768
version: 1
top level: 4-SD
location: https://synbiohub.org/public/igem/BBa_J05040/annotation1706768/range1706768/1
component: BBa_J05217

https://synbiohub.org/public/igem/BBa_J05040/annotation1706768/range1706768/1
type: Range
persistent identity: range1706768
display id: range1706768
version: 1
top level: 4-SD
direction: direction/forward
start: 820
end: 860
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1706773
display id: annotation1706773
version: 1
top level: 4-SD
location: https://synbiohub.org/public/igem/BBa_J05040/annotation1706773/range1706773/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_J05040/annotation1706773/range1706773/1
type: Range
persistent identity: range1706773
display id: range1706773
version: 1
top level: 4-SD
direction: direction/forward
start: 869
end: 880
orientation: inline

BBa_J05110
type: SequenceAnnotation
persistent identity: annotation1706777
display id: annotation1706777
version: 1
top level: 4-SD
location: https://synbiohub.org/public/igem/BBa_J05040/annotation1706777/range1706777/1
component: BBa_J05110

https://synbiohub.org/public/igem/BBa_J05040/annotation1706777/range1706777/1
type: Range
persistent identity: range1706777
display id: range1706777
version: 1
top level: 4-SD
direction: direction/forward
start: 887
end: 1327
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1706783
display id: annotation1706783
version: 1
top level: 4-SD
location: https://synbiohub.org/public/igem/BBa_J05040/annotation1706783/range1706783/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_J05040/annotation1706783/range1706783/1
type: Range
persistent identity: range1706783
display id: range1706783
version: 1
top level: 4-SD
direction: direction/forward
start: 1361
end: 1440
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1706793
display id: annotation1706793
version: 1
top level: 4-SD
location: https://synbiohub.org/public/igem/BBa_J05040/annotation1706793/range1706793/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_J05040/annotation1706793/range1706793/1
type: Range
persistent identity: range1706793
display id: range1706793
version: 1
top level: 4-SD
direction: direction/forward
start: 1449
end: 1489
orientation: inline

BBa_I12007
type: SequenceAnnotation
persistent identity: annotation1706813
display id: annotation1706813
version: 1
top level: 4-SD
location: https://synbiohub.org/public/igem/BBa_J05040/annotation1706813/range1706813/1
component: BBa_I12007

https://synbiohub.org/public/igem/BBa_J05040/annotation1706813/range1706813/1
type: Range
persistent identity: range1706813
display id: range1706813
version: 1
top level: 4-SD
direction: direction/forward
start: 1498
end: 1579
orientation: inline

BBa_J05217
type: SequenceAnnotation
persistent identity: annotation1706815
display id: annotation1706815
version: 1
top level: 4-SD
location: https://synbiohub.org/public/igem/BBa_J05040/annotation1706815/range1706815/1
component: BBa_J05217

https://synbiohub.org/public/igem/BBa_J05040/annotation1706815/range1706815/1
type: Range
persistent identity: range1706815
display id: range1706815
version: 1
top level: 4-SD
direction: direction/forward
start: 1588
end: 1628
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1706820
display id: annotation1706820
version: 1
top level: 4-SD
location: https://synbiohub.org/public/igem/BBa_J05040/annotation1706820/range1706820/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_J05040/annotation1706820/range1706820/1
type: Range
persistent identity: range1706820
display id: range1706820
version: 1
top level: 4-SD
direction: direction/forward
start: 1637
end: 1648
orientation: inline

BBa_J05110
type: SequenceAnnotation
persistent identity: annotation1706824
display id: annotation1706824
version: 1
top level: 4-SD
location: https://synbiohub.org/public/igem/BBa_J05040/annotation1706824/range1706824/1
component: BBa_J05110

https://synbiohub.org/public/igem/BBa_J05040/annotation1706824/range1706824/1
type: Range
persistent identity: range1706824
display id: range1706824
version: 1
top level: 4-SD
direction: direction/forward
start: 1655
end: 2095
orientation: inline

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation1706833
display id: annotation1706833
version: 1
top level: 4-SD
location: https://synbiohub.org/public/igem/BBa_J05040/annotation1706833/range1706833/1
component: BBa_B0030

https://synbiohub.org/public/igem/BBa_J05040/annotation1706833/range1706833/1
type: Range
persistent identity: range1706833
display id: range1706833
version: 1
top level: 4-SD
direction: direction/forward
start: 2129
end: 2143
orientation: inline

BBa_E0040
type: SequenceAnnotation
persistent identity: annotation1706836
display id: annotation1706836
version: 1
top level: 4-SD
location: https://synbiohub.org/public/igem/BBa_J05040/annotation1706836/range1706836/1
component: BBa_E0040

https://synbiohub.org/public/igem/BBa_J05040/annotation1706836/range1706836/1
type: Range
persistent identity: range1706836
display id: range1706836
version: 1
top level: 4-SD
direction: direction/forward
start: 2150
end: 2869
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1706841
display id: annotation1706841
version: 1
top level: 4-SD
location: https://synbiohub.org/public/igem/BBa_J05040/annotation1706841/range1706841/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_J05040/annotation1706841/range1706841/1
type: Range
persistent identity: range1706841
display id: range1706841
version: 1
top level: 4-SD
direction: direction/forward
start: 2878
end: 2957
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1706851
display id: annotation1706851
version: 1
top level: 4-SD
location: https://synbiohub.org/public/igem/BBa_J05040/annotation1706851/range1706851/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_J05040/annotation1706851/range1706851/1
type: Range
persistent identity: range1706851
display id: range1706851
version: 1
top level: 4-SD
direction: direction/forward
start: 2966
end: 3006
orientation: inline

BBa_R0051
type: SequenceAnnotation
persistent identity: annotation1706866
display id: annotation1706866
version: 1
top level: 4-SD
location: https://synbiohub.org/public/igem/BBa_J05040/annotation1706866/range1706866/1
component: BBa_R0051

https://synbiohub.org/public/igem/BBa_J05040/annotation1706866/range1706866/1
type: Range
persistent identity: range1706866
display id: range1706866
version: 1
top level: 4-SD
direction: direction/forward
start: 3015
end: 3063
orientation: inline

BBa_J05218
type: SequenceAnnotation
persistent identity: annotation1706871
display id: annotation1706871
version: 1
top level: 4-SD
location: https://synbiohub.org/public/igem/BBa_J05040/annotation1706871/range1706871/1
component: BBa_J05218

https://synbiohub.org/public/igem/BBa_J05040/annotation1706871/range1706871/1
type: Range
persistent identity: range1706871
display id: range1706871
version: 1
top level: 4-SD
direction: direction/forward
start: 3072
end: 3112
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1706876
display id: annotation1706876
version: 1
top level: 4-SD
location: https://synbiohub.org/public/igem/BBa_J05040/annotation1706876/range1706876/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_J05040/annotation1706876/range1706876/1
type: Range
persistent identity: range1706876
display id: range1706876
version: 1
top level: 4-SD
direction: direction/forward
start: 3121
end: 3132
orientation: inline

BBa_J05111
type: SequenceAnnotation
persistent identity: annotation1706880
display id: annotation1706880
version: 1
top level: 4-SD
location: https://synbiohub.org/public/igem/BBa_J05040/annotation1706880/range1706880/1
component: BBa_J05111

https://synbiohub.org/public/igem/BBa_J05040/annotation1706880/range1706880/1
type: Range
persistent identity: range1706880
display id: range1706880
version: 1
top level: 4-SD
direction: direction/forward
start: 3139
end: 3570
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1706886
display id: annotation1706886
version: 1
top level: 4-SD
location: https://synbiohub.org/public/igem/BBa_J05040/annotation1706886/range1706886/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_J05040/annotation1706886/range1706886/1
type: Range
persistent identity: range1706886
display id: range1706886
version: 1
top level: 4-SD
direction: direction/forward
start: 3604
end: 3683
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1706896
display id: annotation1706896
version: 1
top level: 4-SD
location: https://synbiohub.org/public/igem/BBa_J05040/annotation1706896/range1706896/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_J05040/annotation1706896/range1706896/1
type: Range
persistent identity: range1706896
display id: range1706896
version: 1
top level: 4-SD
direction: direction/forward
start: 3692
end: 3732
orientation: inline

EPD
type: ComponentDefinition
persistent identity: BBa J05500
display id: BBa_J05500
version: 1
was generated by: iGEM to SBOL conversion
description: Sensing Device A (cI)
created: 2005-09-20T11:00:00Z
modified: 2015-08-31T04:08:15Z
mutable provenance: Registry 7.05
owned by: james
owned by: myers
mutable description: The input system for the iGEM2005 ETH "Counter" project: its purpose is to regulate cI production dependent on an external event. It works somehow like an inverter: STATE 1: when IPTG is absent, the constitutively produced lacI protein will block the lacI+pL promoter so that no cI is produced. Thus downstream (not included in this composite) the Pr promoter will be constitutively active, producing some zinc finger proteins, while Prm+ will have low or no activity. STATE 2: when IPTG is present, it will remove the lacI protein from the lacI+pL promoter and cI is produced. Thus downstream (not included in this composite) the Pr promoter will be repressed by cI, while Prm+ will be activated, producing some other zinc finger proteins
discontinued: false
dominant: false
experience: experience/None
group u list: _34_
m user id: 0
owner id: 398
owning group id: 34
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: For more details see Sensing Device and Event Processing Device documentation.
star: false
creator: ETH Zurich iGEM Team 2005
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
component: BBa_C0051
component: BBa_R0040
component: BBa_B0012
component: BBa_C0012
component: BBa_B0034
component: BBa_B0010
component: BBa_R0011
sequence annotation: BBa_R0040
sequence annotation: BBa_B0034
sequence annotation: BBa_C0012
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence annotation: BBa_R0011
sequence annotation: BBa_C0051
sequence: https://synbiohub.org/public/igem/BBa_J05500_sequence/1

BBa_C0051
type: Component
persistent identity: component1702187
display id: component1702187
version: 1
top level: EPD
definition: cI lam
access: public

BBa_R0040
type: Component
persistent identity: component1702119
display id: component1702119
version: 1
top level: EPD
definition: p(tetR)
access: public

BBa_B0012
type: Component
persistent identity: component1702203
display id: component1702203
version: 1
top level: EPD
definition: BBa_B0012
access: public

BBa_C0012
type: Component
persistent identity: component1702137
display id: component1702137
version: 1
top level: EPD
definition: lacI
access: public

BBa_B0034
type: Component
persistent identity: component1702127
display id: component1702127
version: 1
top level: EPD
definition: BBa_B0034
access: public

BBa_B0012
type: Component
persistent identity: component1702154
display id: component1702154
version: 1
top level: EPD
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component1702144
display id: component1702144
version: 1
top level: EPD
definition: BBa_B0010
access: public

BBa_B0034
type: Component
persistent identity: component1702177
display id: component1702177
version: 1
top level: EPD
definition: BBa_B0034
access: public

BBa_R0011
type: Component
persistent identity: component1702168
display id: component1702168
version: 1
top level: EPD
definition: lacI+pL
access: public

BBa_B0010
type: Component
persistent identity: component1702193
display id: component1702193
version: 1
top level: EPD
definition: BBa_B0010
access: public

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation1702119
display id: annotation1702119
version: 1
top level: EPD
location: https://synbiohub.org/public/igem/BBa_J05500/annotation1702119/range1702119/1
component: BBa_R0040

https://synbiohub.org/public/igem/BBa_J05500/annotation1702119/range1702119/1
type: Range
persistent identity: range1702119
display id: range1702119
version: 1
top level: EPD
direction: direction/forward
start: 1
end: 54
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1702127
display id: annotation1702127
version: 1
top level: EPD
location: https://synbiohub.org/public/igem/BBa_J05500/annotation1702127/range1702127/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_J05500/annotation1702127/range1702127/1
type: Range
persistent identity: range1702127
display id: range1702127
version: 1
top level: EPD
direction: direction/forward
start: 63
end: 74
orientation: inline

BBa_C0012
type: SequenceAnnotation
persistent identity: annotation1702137
display id: annotation1702137
version: 1
top level: EPD
location: https://synbiohub.org/public/igem/BBa_J05500/annotation1702137/range1702137/1
component: BBa_C0012

https://synbiohub.org/public/igem/BBa_J05500/annotation1702137/range1702137/1
type: Range
persistent identity: range1702137
display id: range1702137
version: 1
top level: EPD
direction: direction/forward
start: 81
end: 1208
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1702144
display id: annotation1702144
version: 1
top level: EPD
location: https://synbiohub.org/public/igem/BBa_J05500/annotation1702144/range1702144/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_J05500/annotation1702144/range1702144/1
type: Range
persistent identity: range1702144
display id: range1702144
version: 1
top level: EPD
direction: direction/forward
start: 1242
end: 1321
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1702154
display id: annotation1702154
version: 1
top level: EPD
location: https://synbiohub.org/public/igem/BBa_J05500/annotation1702154/range1702154/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_J05500/annotation1702154/range1702154/1
type: Range
persistent identity: range1702154
display id: range1702154
version: 1
top level: EPD
direction: direction/forward
start: 1330
end: 1370
orientation: inline

BBa_R0011
type: SequenceAnnotation
persistent identity: annotation1702168
display id: annotation1702168
version: 1
top level: EPD
location: https://synbiohub.org/public/igem/BBa_J05500/annotation1702168/range1702168/1
component: BBa_R0011

https://synbiohub.org/public/igem/BBa_J05500/annotation1702168/range1702168/1
type: Range
persistent identity: range1702168
display id: range1702168
version: 1
top level: EPD
direction: direction/forward
start: 1379
end: 1432
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1702177
display id: annotation1702177
version: 1
top level: EPD
location: https://synbiohub.org/public/igem/BBa_J05500/annotation1702177/range1702177/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_J05500/annotation1702177/range1702177/1
type: Range
persistent identity: range1702177
display id: range1702177
version: 1
top level: EPD
direction: direction/forward
start: 1442
end: 1453
orientation: inline

BBa_C0051
type: SequenceAnnotation
persistent identity: annotation1702187
display id: annotation1702187
version: 1
top level: EPD
location: https://synbiohub.org/public/igem/BBa_J05500/annotation1702187/range1702187/1
component: BBa_C0051

https://synbiohub.org/public/igem/BBa_J05500/annotation1702187/range1702187/1
type: Range
persistent identity: range1702187
display id: range1702187
version: 1
top level: EPD
direction: direction/forward
start: 1460
end: 2209
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1702193
display id: annotation1702193
version: 1
top level: EPD
location: https://synbiohub.org/public/igem/BBa_J05500/annotation1702193/range1702193/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_J05500/annotation1702193/range1702193/1
type: Range
persistent identity: range1702193
display id: range1702193
version: 1
top level: EPD
direction: direction/forward
start: 2243
end: 2322
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1702203
display id: annotation1702203
version: 1
top level: EPD
location: https://synbiohub.org/public/igem/BBa_J05500/annotation1702203/range1702203/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_J05500/annotation1702203/range1702203/1
type: Range
persistent identity: range1702203
display id: range1702203
version: 1
top level: EPD
direction: direction/forward
start: 2331
end: 2371
orientation: inline

R1
type: ComponentDefinition
persistent identity: BBa J05108
display id: BBa_J05108
version: 1
was generated by: iGEM to SBOL conversion
description: Repressor R1-CREBH
created: 2005-09-03T11:00:00Z
modified: 2015-08-31T04:08:15Z
owned by: james
owned by: myers
mutable description: HR1-CREBH (GGA GGG GAC) => (GTC CCC TCC GGA GGG GAC)
discontinued: false
dominant: false
group u list: _34_
m user id: 0
owner id: 412
owning group id: 34
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Alexander Roth
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Finger 1
sequence annotation: ZF-GAC
sequence annotation: Finger 2
sequence annotation: ZF-GGG
sequence annotation: Finger 3
sequence annotation: ZF-GGA
sequence annotation: Help:Barcodes
sequence: https://synbiohub.org/public/igem/BBa_J05108_sequence/1

Finger 1
type: SequenceAnnotation
persistent identity: annotation1730068
display id: annotation1730068
version: 1
top level: R1
location: https://synbiohub.org/public/igem/BBa_J05108/annotation1730068/range1730068/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_J05108/annotation1730068/range1730068/1
type: Range
persistent identity: range1730068
display id: range1730068
version: 1
top level: R1
direction: direction/forward
start: 22
end: 105
orientation: inline

ZF-GAC
type: SequenceAnnotation
persistent identity: annotation1730069
display id: annotation1730069
version: 1
top level: R1
location: https://synbiohub.org/public/igem/BBa_J05108/annotation1730069/range1730069/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_J05108/annotation1730069/range1730069/1
type: Range
persistent identity: range1730069
display id: range1730069
version: 1
top level: R1
direction: direction/forward
start: 55
end: 78
orientation: inline

Finger 2
type: SequenceAnnotation
persistent identity: annotation1730070
display id: annotation1730070
version: 1
top level: R1
location: https://synbiohub.org/public/igem/BBa_J05108/annotation1730070/range1730070/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_J05108/annotation1730070/range1730070/1
type: Range
persistent identity: range1730070
display id: range1730070
version: 1
top level: R1
direction: direction/forward
start: 106
end: 189
orientation: inline

ZF-GGG
type: SequenceAnnotation
persistent identity: annotation1730071
display id: annotation1730071
version: 1
top level: R1
location: https://synbiohub.org/public/igem/BBa_J05108/annotation1730071/range1730071/1
role: feature/binding
role: SO:0001091

https://synbiohub.org/public/igem/BBa_J05108/annotation1730071/range1730071/1
type: Range
persistent identity: range1730071
display id: range1730071
version: 1
top level: R1
direction: direction/forward
start: 139
end: 162
orientation: inline

Finger 3
type: SequenceAnnotation
persistent identity: annotation1730072
display id: annotation1730072
version: 1
top level: R1
location: https://synbiohub.org/public/igem/BBa_J05108/annotation1730072/range1730072/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_J05108/annotation1730072/range1730072/1
type: Range
persistent identity: range1730072
display id: range1730072
version: 1
top level: R1
direction: direction/forward
start: 190
end: 273
orientation: inline

ZF-GGA
type: SequenceAnnotation
persistent identity: annotation1730073
display id: annotation1730073
version: 1
top level: R1
location: https://synbiohub.org/public/igem/BBa_J05108/annotation1730073/range1730073/1
role: feature/binding
role: SO:0001091

https://synbiohub.org/public/igem/BBa_J05108/annotation1730073/range1730073/1
type: Range
persistent identity: range1730073
display id: range1730073
version: 1
top level: R1
direction: direction/forward
start: 223
end: 246
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2214041
display id: annotation2214041
version: 1
top level: R1
location: https://synbiohub.org/public/igem/BBa_J05108/annotation2214041/range2214041/1
role: SO:0000807
role: feature/barcode

https://synbiohub.org/public/igem/BBa_J05108/annotation2214041/range2214041/1
type: Range
persistent identity: range2214041
display id: range2214041
version: 1
top level: R1
direction: direction/forward
start: 436
end: 460
orientation: inline

BBa_J05215
type: ComponentDefinition
persistent identity: BBa J05215
display id: BBa_J05215
version: 1
was generated by: iGEM to SBOL conversion
description: Regulator for R1-CREBH
created: 2005-09-07T11:00:00Z
modified: 2015-08-31T04:08:15Z
owned by: james
owned by: myers
mutable description: binding sites for R3-ATF6 and R2-YAP7
discontinued: false
dominant: false
group u list: _34_
m user id: 0
owner id: 412
owning group id: 34
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Alexander Roth
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_J05215_sequence/1

BBa_B0034
type: ComponentDefinition
persistent identity: BBa B0034
display id: BBa_B0034
version: 1
was generated by: iGEM to SBOL conversion
description: RBS (Elowitz 1999) -- defines RBS efficiency
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: RBS based on Elowitz repressilator.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: conserved
sequence: https://synbiohub.org/public/igem/BBa_B0034_sequence/1

conserved
type: SequenceAnnotation
persistent identity: annotation23325
display id: annotation23325
version: 1
top level: BBa_B0034
location: https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
role: SO:0000330
role: feature/conserved

https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
type: Range
persistent identity: range23325
display id: range23325
version: 1
top level: BBa_B0034
direction: direction/forward
start: 5
end: 8
orientation: inline

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: SO:0000313
role: feature/stem loop

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: feature/polya
role: SO:0000553

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

Prm +
type: ComponentDefinition
persistent identity: BBa I12007
display id: BBa_I12007
version: 1
was generated by: iGEM to SBOL conversion
description: Modified lambda Prm promoter (OR-3 obliterated)
created: 2004-07-14T11:00:00Z
modified: 2015-08-31T04:07:31Z
mutable provenance: Shih and Gussin (1983)
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Lambda Prm promoter modified to be activated but not repressed by the lambda repressor (cI)
discontinued: false
dominant: true
experience: experience/None
group u list: _3_
m user id: 0
owner id: 103
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: In wild type lambda phage, the OR3 site (-27 to -11) reads "tatcccttgcggtgata" on the sense strand of DNA. To prevent lambda repressor (cI) from binding to this site, the 4th through 10th nt of OR3 were replaced by the 7 nt between OR2 and OR1, "aaatagt" (-57 to -51), which in effect mutates 5 nt of the promoter. These nt were chosen to be about halfway between the -10 and -35 boxes of the promoter. Furthermore, since these nt already act as a spacer in wild-type phage, it is hoped they will not create undesired interactions.
star: true
creator: Hans
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: OR1
sequence annotation: OR2
sequence annotation: -35
sequence annotation: mutate to obliterate OR3
sequence annotation: -10
sequence: https://synbiohub.org/public/igem/BBa_I12007_sequence/1

OR1
type: SequenceAnnotation
persistent identity: annotation937701
display id: annotation937701
version: 1
top level: Prm +
location: https://synbiohub.org/public/igem/BBa_I12007/annotation937701/range937701/1
role: feature/binding
role: SO:0001091

https://synbiohub.org/public/igem/BBa_I12007/annotation937701/range937701/1
type: Range
persistent identity: range937701
display id: range937701
version: 1
top level: Prm +
direction: direction/forward
start: 9
end: 25
orientation: inline

OR2
type: SequenceAnnotation
persistent identity: annotation937702
display id: annotation937702
version: 1
top level: Prm +
location: https://synbiohub.org/public/igem/BBa_I12007/annotation937702/range937702/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_I12007/annotation937702/range937702/1
type: Range
persistent identity: range937702
display id: range937702
version: 1
top level: Prm +
direction: direction/forward
start: 33
end: 49
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation937703
display id: annotation937703
version: 1
top level: Prm +
location: https://synbiohub.org/public/igem/BBa_I12007/annotation937703/range937703/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_I12007/annotation937703/range937703/1
type: Range
persistent identity: range937703
display id: range937703
version: 1
top level: Prm +
direction: direction/forward
start: 48
end: 53
orientation: inline

mutate to obliterate OR3
type: SequenceAnnotation
persistent identity: annotation937704
display id: annotation937704
version: 1
top level: Prm +
location: https://synbiohub.org/public/igem/BBa_I12007/annotation937704/range937704/1
role: feature/mutation
role: SO:0001059

https://synbiohub.org/public/igem/BBa_I12007/annotation937704/range937704/1
type: Range
persistent identity: range937704
display id: range937704
version: 1
top level: Prm +
direction: direction/forward
start: 59
end: 65
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation937705
display id: annotation937705
version: 1
top level: Prm +
location: https://synbiohub.org/public/igem/BBa_I12007/annotation937705/range937705/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_I12007/annotation937705/range937705/1
type: Range
persistent identity: range937705
display id: range937705
version: 1
top level: Prm +
direction: direction/forward
start: 71
end: 76
orientation: inline

R2
type: ComponentDefinition
persistent identity: BBa J05110
display id: BBa_J05110
version: 1
was generated by: iGEM to SBOL conversion
description: Repressor R2-YAP7
created: 2005-09-03T11:00:00Z
modified: 2015-08-31T04:08:15Z
owned by: james
owned by: myers
mutable description: R2-YAP7 (GGA GGC GGG) => (CCC GCC TCC GGA GGC GGG)
discontinued: false
dominant: false
group u list: _34_
m user id: 0
owner id: 412
owning group id: 34
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Alexander Roth
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: Finger 1
sequence annotation: ZF-GGG
sequence annotation: Finger 2
sequence annotation: ZF-GGC
sequence annotation: Finger 3
sequence annotation: ZF-GGA
sequence annotation: Help:Barcodes
sequence: https://synbiohub.org/public/igem/BBa_J05110_sequence/1

Finger 1
type: SequenceAnnotation
persistent identity: annotation1730098
display id: annotation1730098
version: 1
top level: R2
location: https://synbiohub.org/public/igem/BBa_J05110/annotation1730098/range1730098/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_J05110/annotation1730098/range1730098/1
type: Range
persistent identity: range1730098
display id: range1730098
version: 1
top level: R2
direction: direction/forward
start: 22
end: 105
orientation: inline

ZF-GGG
type: SequenceAnnotation
persistent identity: annotation1730099
display id: annotation1730099
version: 1
top level: R2
location: https://synbiohub.org/public/igem/BBa_J05110/annotation1730099/range1730099/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_J05110/annotation1730099/range1730099/1
type: Range
persistent identity: range1730099
display id: range1730099
version: 1
top level: R2
direction: direction/forward
start: 55
end: 78
orientation: inline

Finger 2
type: SequenceAnnotation
persistent identity: annotation1730100
display id: annotation1730100
version: 1
top level: R2
location: https://synbiohub.org/public/igem/BBa_J05110/annotation1730100/range1730100/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_J05110/annotation1730100/range1730100/1
type: Range
persistent identity: range1730100
display id: range1730100
version: 1
top level: R2
direction: direction/forward
start: 106
end: 189
orientation: inline

ZF-GGC
type: SequenceAnnotation
persistent identity: annotation1730101
display id: annotation1730101
version: 1
top level: R2
location: https://synbiohub.org/public/igem/BBa_J05110/annotation1730101/range1730101/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_J05110/annotation1730101/range1730101/1
type: Range
persistent identity: range1730101
display id: range1730101
version: 1
top level: R2
direction: direction/forward
start: 139
end: 162
orientation: inline

Finger 3
type: SequenceAnnotation
persistent identity: annotation1730102
display id: annotation1730102
version: 1
top level: R2
location: https://synbiohub.org/public/igem/BBa_J05110/annotation1730102/range1730102/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_J05110/annotation1730102/range1730102/1
type: Range
persistent identity: range1730102
display id: range1730102
version: 1
top level: R2
direction: direction/forward
start: 190
end: 273
orientation: inline

ZF-GGA
type: SequenceAnnotation
persistent identity: annotation1730103
display id: annotation1730103
version: 1
top level: R2
location: https://synbiohub.org/public/igem/BBa_J05110/annotation1730103/range1730103/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_J05110/annotation1730103/range1730103/1
type: Range
persistent identity: range1730103
display id: range1730103
version: 1
top level: R2
direction: direction/forward
start: 223
end: 246
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2214043
display id: annotation2214043
version: 1
top level: R2
location: https://synbiohub.org/public/igem/BBa_J05110/annotation2214043/range2214043/1
role: feature/barcode
role: SO:0000807

https://synbiohub.org/public/igem/BBa_J05110/annotation2214043/range2214043/1
type: Range
persistent identity: range2214043
display id: range2214043
version: 1
top level: R2
direction: direction/forward
start: 442
end: 466
orientation: inline

BBa_J05217
type: ComponentDefinition
persistent identity: BBa J05217
display id: BBa_J05217
version: 1
was generated by: iGEM to SBOL conversion
description: Regulator for R2-YAP7
created: 2005-09-07T11:00:00Z
modified: 2015-08-31T04:08:15Z
owned by: james
owned by: myers
mutable description: binding sites for R3-ATF6 and R4-cMaf
discontinued: false
dominant: false
group u list: _34_
m user id: 0
owner id: 412
owning group id: 34
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Alexander Roth
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
sequence: https://synbiohub.org/public/igem/BBa_J05217_sequence/1

BBa_J05218
type: ComponentDefinition
persistent identity: BBa J05218
display id: BBa_J05218
version: 1
was generated by: iGEM to SBOL conversion
description: Regulator for R4-cMaf
created: 2005-09-07T11:00:00Z
modified: 2015-08-31T04:08:15Z
owned by: james
owned by: myers
mutable description: binding sites for R1-CREBH and R2-YAP7
discontinued: false
dominant: false
group u list: _34_
m user id: 0
owner id: 412
owning group id: 34
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Alexander Roth
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
sequence: https://synbiohub.org/public/igem/BBa_J05218_sequence/1

cI lam
type: ComponentDefinition
persistent identity: BBa R0051
display id: BBa_R0051
version: 1
was generated by: iGEM to SBOL conversion
description: promoter (lambda cI regulated)
created: 2003-01-31T12:00:00Z
modified: 2015-05-08T01:14:14Z
mutable provenance: A synthetic oscillatory network of transcriptional regulators , Elowitz M.B. , Leibler S., Nature(403),335-38: 2000
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: The cI regulated promoter is based on the pR promtoer from bacteriohage lambda. The promoter has two two DNA binding sites for lambda cI repressor <bb_part>BBa_C0051</bb_part>. cI binding results in repression of transcription. The specific sequence used here is based on the cI repressible promoter used in the Elowitz repressilator (and references therein).
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: In order to address concerns about the promoter transcribing in the reverse direction, we have removed the -35 and -10 signals responsible for the promoter activity in the reverse direction. (More details needed here! DE, 2/24/03) Incompatible with host expressing cI repressor.
star: true
creator: Vinay S Mahajan, Brian Chow, Peter Carr, Voichita Marinescu and Alexander D. Wissner-Gross
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
sequence annotation: BBa_R0051
sequence annotation: OR2
sequence annotation: -35
sequence annotation: OR1
sequence annotation: -10
sequence: https://synbiohub.org/public/igem/BBa_R0051_sequence/1

BBa_R0051
type: SequenceAnnotation
persistent identity: annotation7067
display id: annotation7067
version: 1
top level: cI lam
location: https://synbiohub.org/public/igem/BBa_R0051/annotation7067/range7067/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_R0051/annotation7067/range7067/1
type: Range
persistent identity: range7067
display id: range7067
version: 1
top level: cI lam
direction: direction/forward
start: 1
end: 49
orientation: inline

OR2
type: SequenceAnnotation
persistent identity: annotation2025
display id: annotation2025
version: 1
top level: cI lam
location: https://synbiohub.org/public/igem/BBa_R0051/annotation2025/range2025/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_R0051/annotation2025/range2025/1
type: Range
persistent identity: range2025
display id: range2025
version: 1
top level: cI lam
direction: direction/forward
start: 1
end: 17
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation2023
display id: annotation2023
version: 1
top level: cI lam
location: https://synbiohub.org/public/igem/BBa_R0051/annotation2023/range2023/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_R0051/annotation2023/range2023/1
type: Range
persistent identity: range2023
display id: range2023
version: 1
top level: cI lam
direction: direction/forward
start: 15
end: 20
orientation: inline

OR1
type: SequenceAnnotation
persistent identity: annotation2024
display id: annotation2024
version: 1
top level: cI lam
location: https://synbiohub.org/public/igem/BBa_R0051/annotation2024/range2024/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_R0051/annotation2024/range2024/1
type: Range
persistent identity: range2024
display id: range2024
version: 1
top level: cI lam
direction: direction/forward
start: 25
end: 41
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation2022
display id: annotation2022
version: 1
top level: cI lam
location: https://synbiohub.org/public/igem/BBa_R0051/annotation2022/range2022/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_R0051/annotation2022/range2022/1
type: Range
persistent identity: range2022
display id: range2022
version: 1
top level: cI lam
direction: direction/forward
start: 38
end: 43
orientation: inline

BBa_B0030
type: ComponentDefinition
persistent identity: BBa B0030
display id: BBa_B0030
version: 1
was generated by: iGEM to SBOL conversion
description: RBS.1 (strong) -- modified from R. Weiss
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Strong RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0032</bb_part>, <bb_part>BBa_B0033</bb_part>.
discontinued: false
dominant: true
experience: experience/Works
group u list: _44_46_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("orig" in figure 4-14 of Ron Weiss thesis). No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: RBS-1\Strong
sequence annotation: BBa_B0030
sequence annotation: RBS
sequence: https://synbiohub.org/public/igem/BBa_B0030_sequence/1

RBS-1\Strong
type: SequenceAnnotation
persistent identity: annotation1701
display id: annotation1701
version: 1
top level: BBa_B0030
location: https://synbiohub.org/public/igem/BBa_B0030/annotation1701/range1701/1
role: SO:0000139
role: feature/rbs

https://synbiohub.org/public/igem/BBa_B0030/annotation1701/range1701/1
type: Range
persistent identity: range1701
display id: range1701
version: 1
top level: BBa_B0030
direction: direction/forward
start: 1
end: 15
orientation: inline

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation7025
display id: annotation7025
version: 1
top level: BBa_B0030
location: https://synbiohub.org/public/igem/BBa_B0030/annotation7025/range7025/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0030/annotation7025/range7025/1
type: Range
persistent identity: range7025
display id: range7025
version: 1
top level: BBa_B0030
direction: direction/forward
start: 1
end: 15
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation1702
display id: annotation1702
version: 1
top level: BBa_B0030
location: https://synbiohub.org/public/igem/BBa_B0030/annotation1702/range1702/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_B0030/annotation1702/range1702/1
type: Range
persistent identity: range1702
display id: range1702
version: 1
top level: BBa_B0030
direction: direction/forward
start: 8
end: 12
orientation: inline

GFP
type: ComponentDefinition
persistent identity: BBa E0040
display id: BBa_E0040
version: 1
was generated by: iGEM to SBOL conversion
description: green fluorescent protein derived from jellyfish Aequeora victoria wild-type GFP (SwissProt: P42212
created: 2004-09-29T11:00:00Z
modified: 2016-01-26T02:09:38Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: GFP (mut3b) [note that this part does not have a barcode]
discontinued: false
dominant: true
experience: experience/Works
group u list: _11_1_
m user id: 4206
owner id: 61
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: jcbraff
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: GFP protein
sequence: https://synbiohub.org/public/igem/BBa_E0040_sequence/1

GFP protein
type: SequenceAnnotation
persistent identity: annotation1934520
display id: annotation1934520
version: 1
top level: GFP
location: https://synbiohub.org/public/igem/BBa_E0040/annotation1934520/range1934520/1
role: SO:0000316
role: feature/protein

https://synbiohub.org/public/igem/BBa_E0040/annotation1934520/range1934520/1
type: Range
persistent identity: range1934520
display id: range1934520
version: 1
top level: GFP
direction: direction/forward
start: 1
end: 720
orientation: inline

R4
type: ComponentDefinition
persistent identity: BBa J05111
display id: BBa_J05111
version: 1
was generated by: iGEM to SBOL conversion
description: Repressor R4-cMaf
created: 2005-09-03T11:00:00Z
modified: 2015-08-31T04:08:15Z
owned by: james
owned by: myers
mutable description: R4-cMaf (GGG GCC GGA) => (TCC GGC CCC GGG GCC GGA)
discontinued: false
dominant: false
group u list: _34_
m user id: 0
owner id: 412
owning group id: 34
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Alexander Roth
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: Finger 1
sequence annotation: ZF-GGA
sequence annotation: Finger 2
sequence annotation: ZF-GCC
sequence annotation: Finger 3
sequence annotation: ZF-GGG
sequence annotation: Help:Barcodes
sequence: https://synbiohub.org/public/igem/BBa_J05111_sequence/1

Finger 1
type: SequenceAnnotation
persistent identity: annotation1730113
display id: annotation1730113
version: 1
top level: R4
location: https://synbiohub.org/public/igem/BBa_J05111/annotation1730113/range1730113/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_J05111/annotation1730113/range1730113/1
type: Range
persistent identity: range1730113
display id: range1730113
version: 1
top level: R4
direction: direction/forward
start: 22
end: 105
orientation: inline

ZF-GGA
type: SequenceAnnotation
persistent identity: annotation1730114
display id: annotation1730114
version: 1
top level: R4
location: https://synbiohub.org/public/igem/BBa_J05111/annotation1730114/range1730114/1
role: feature/binding
role: SO:0001091

https://synbiohub.org/public/igem/BBa_J05111/annotation1730114/range1730114/1
type: Range
persistent identity: range1730114
display id: range1730114
version: 1
top level: R4
direction: direction/forward
start: 55
end: 78
orientation: inline

Finger 2
type: SequenceAnnotation
persistent identity: annotation1730115
display id: annotation1730115
version: 1
top level: R4
location: https://synbiohub.org/public/igem/BBa_J05111/annotation1730115/range1730115/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_J05111/annotation1730115/range1730115/1
type: Range
persistent identity: range1730115
display id: range1730115
version: 1
top level: R4
direction: direction/forward
start: 106
end: 189
orientation: inline

ZF-GCC
type: SequenceAnnotation
persistent identity: annotation1730116
display id: annotation1730116
version: 1
top level: R4
location: https://synbiohub.org/public/igem/BBa_J05111/annotation1730116/range1730116/1
role: feature/binding
role: SO:0001091

https://synbiohub.org/public/igem/BBa_J05111/annotation1730116/range1730116/1
type: Range
persistent identity: range1730116
display id: range1730116
version: 1
top level: R4
direction: direction/forward
start: 139
end: 162
orientation: inline

Finger 3
type: SequenceAnnotation
persistent identity: annotation1730117
display id: annotation1730117
version: 1
top level: R4
location: https://synbiohub.org/public/igem/BBa_J05111/annotation1730117/range1730117/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_J05111/annotation1730117/range1730117/1
type: Range
persistent identity: range1730117
display id: range1730117
version: 1
top level: R4
direction: direction/forward
start: 190
end: 273
orientation: inline

ZF-GGG
type: SequenceAnnotation
persistent identity: annotation1730118
display id: annotation1730118
version: 1
top level: R4
location: https://synbiohub.org/public/igem/BBa_J05111/annotation1730118/range1730118/1
role: feature/binding
role: SO:0001091

https://synbiohub.org/public/igem/BBa_J05111/annotation1730118/range1730118/1
type: Range
persistent identity: range1730118
display id: range1730118
version: 1
top level: R4
direction: direction/forward
start: 223
end: 246
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2214044
display id: annotation2214044
version: 1
top level: R4
location: https://synbiohub.org/public/igem/BBa_J05111/annotation2214044/range2214044/1
role: SO:0000807
role: feature/barcode

https://synbiohub.org/public/igem/BBa_J05111/annotation2214044/range2214044/1
type: Range
persistent identity: range2214044
display id: range2214044
version: 1
top level: R4
direction: direction/forward
start: 433
end: 457
orientation: inline

cI lam
type: ComponentDefinition
persistent identity: BBa C0051
display id: BBa_C0051
version: 1
was generated by: iGEM to SBOL conversion
description: cI repressor from E. coli phage lambda (+LVA)
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:23Z
mutable provenance: Elowitz, M. B. Transport, Assembly, and Dynamics in Systems of Interacting Proteins. Thesis, Princeton Univ., Princeton (1999).
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Coding region for the cI repressor based on cI repressor from bacteriophage lambda modified with an LVA tail for rapid degradation of the protein. cI repressor binds to the cI regulator (BBa_R0051).
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: References (unparsed) here: A synthetic oscillatory network of transcriptional regulators , Elowitz M.B. , Leibler S., Nature(403),335-38: 2000 Octamerization of CI repressor is needed for effective repression of PRM and efficient switching from lysogeny. Ian B. Dodd,1 Alison J. Perkins, Daniel Tsemitsidis, and J. Barry Egan , Genes and Development (Vol 15, No. 22) 3013-3022: 2001 References (unparsed) here: A synthetic oscillatory network of transcriptional regulators , Elowitz M.B. , Leibler S., Nature(403),335-38: 2000 Octamerization of CI repressor is needed for effective repression of PRM and efficient switching from lysogeny. Ian B. Dodd,1 Alison J. Perkins, Daniel Tsemitsidis, and J. Barry Egan , Genes and Development (Vol 15, No. 22) 3013-3022: 2001 BBa_C0051 cI repressor is based on the cI repressor from the Elowitz's repressilator. It has been modified to include a rapid degradation LAA tail, and includes the BioBrick standard assembly head and tail restriction sites. The RBS has been removed. The stop codon has been changed from TAA to a double stop codon TAATAA.
star: true
creator: Vinay S Mahajan, Brian Chow, Peter Carr, Voichita Marinescu and Alexander D. Wissner-Gross
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: cI lambda
sequence annotation: LVA
sequence annotation: Help:Barcodes
sequence: https://synbiohub.org/public/igem/BBa_C0051_sequence/1

cI lambda
type: SequenceAnnotation
persistent identity: annotation23334
display id: annotation23334
version: 1
top level: cI lam
location: https://synbiohub.org/public/igem/BBa_C0051/annotation23334/range23334/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_C0051/annotation23334/range23334/1
type: Range
persistent identity: range23334
display id: range23334
version: 1
top level: cI lam
direction: direction/forward
start: 4
end: 711
orientation: inline

LVA
type: SequenceAnnotation
persistent identity: annotation23335
display id: annotation23335
version: 1
top level: cI lam
location: https://synbiohub.org/public/igem/BBa_C0051/annotation23335/range23335/1
role: feature/tag
role: SO:0000324

https://synbiohub.org/public/igem/BBa_C0051/annotation23335/range23335/1
type: Range
persistent identity: range23335
display id: range23335
version: 1
top level: cI lam
direction: direction/forward
start: 712
end: 744
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2213991
display id: annotation2213991
version: 1
top level: cI lam
location: https://synbiohub.org/public/igem/BBa_C0051/annotation2213991/range2213991/1
role: feature/barcode
role: SO:0000807

https://synbiohub.org/public/igem/BBa_C0051/annotation2213991/range2213991/1
type: Range
persistent identity: range2213991
display id: range2213991
version: 1
top level: cI lam
direction: direction/forward
start: 751
end: 775
orientation: inline

p(tetR)
type: ComponentDefinition
persistent identity: BBa R0040
display id: BBa_R0040
version: 1
was generated by: iGEM to SBOL conversion
description: TetR repressible promoter
created: 2003-01-31T12:00:00Z
modified: 2015-05-08T01:14:14Z
mutable provenance: Lutz, R., Bujard, H., Nucleic Acids Research (1997) 25, 1203-1210.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Sequence for pTet inverting regulator driven by the TetR protein.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: BBa_R0040 TetR-Regulated Promoter is based on a cI promoter. It has been modified to include two TetR binding sites and the BioBrick standard assembly head and tail restriction sites.
star: true
creator: June Rhee, Connie Tao, Ty Thomson, Louis Waldman
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: BBa_R0040
sequence annotation: TetR 1
sequence annotation: -35
sequence annotation: TetR 2
sequence annotation: -10
sequence: https://synbiohub.org/public/igem/BBa_R0040_sequence/1

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation1986784
display id: annotation1986784
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986784/range1986784/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_R0040/annotation1986784/range1986784/1
type: Range
persistent identity: range1986784
display id: range1986784
version: 1
top level: p(tetR)
direction: direction/forward
start: 1
end: 54
orientation: inline

TetR 1
type: SequenceAnnotation
persistent identity: annotation1986783
display id: annotation1986783
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986783/range1986783/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_R0040/annotation1986783/range1986783/1
type: Range
persistent identity: range1986783
display id: range1986783
version: 1
top level: p(tetR)
direction: direction/forward
start: 1
end: 19
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1986785
display id: annotation1986785
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986785/range1986785/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_R0040/annotation1986785/range1986785/1
type: Range
persistent identity: range1986785
display id: range1986785
version: 1
top level: p(tetR)
direction: direction/forward
start: 20
end: 25
orientation: inline

TetR 2
type: SequenceAnnotation
persistent identity: annotation1986786
display id: annotation1986786
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986786/range1986786/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_R0040/annotation1986786/range1986786/1
type: Range
persistent identity: range1986786
display id: range1986786
version: 1
top level: p(tetR)
direction: direction/forward
start: 26
end: 44
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1986787
display id: annotation1986787
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986787/range1986787/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_R0040/annotation1986787/range1986787/1
type: Range
persistent identity: range1986787
display id: range1986787
version: 1
top level: p(tetR)
direction: direction/forward
start: 43
end: 48
orientation: inline

lacI
type: ComponentDefinition
persistent identity: BBa C0012
display id: BBa_C0012
version: 1
was generated by: iGEM to SBOL conversion
description: lacI repressor from E. coli (+LVA)
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:23Z
mutable provenance: represillator of Elowitz and Leibler (2000)
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Coding region for the LacI protein with an LVA degradation tail and without an RBS. LacI binds to the pLac regulator <bb_part>BBa_R0010</bb_part> and PLlac01 hybrid regulator <bb_part>BBa_R0011</bb_part> and inhibits transcription. IPTG (Isopropylthiogalactoside) binds to LacI and inhibits its operation, therefore promoting transcription. A rapid degredation tail (LVA) has been added to improve the High to Low performance of this part.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: References (unparsed) here: Elowitz, M.B., Leibler, S. A synthetic oscillatory network of transcriptional regulators. Nature 403, 335-338 (2000). [ELOW00] References (unparsed) here: Elowitz, M.B., Leibler, S. A synthetic oscillatory network of transcriptional regulators. Nature 403, 335-338 (2000). [ELOW00] Sequence taken from the repressilator of Elowitz and Leibler (2000). The obtained sequence was compared to the wild-type sequence for LacI obtained through a database search. The sequence had been modified from the wild-type in that wild-type GTG start was changed to an ATG start (note, actual ORF in E.coli has several GTG starts it would seem). The LVA tag has been added for quicker degradation. Incompatible with systems containing LacI, lactose, or IPTG.
star: true
creator: Grace Kenney, Daniel Shen, Neelaksh Varshney, Samantha Sutton
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: BBa_C0012
sequence annotation: lacI-LVA
sequence annotation: LVA
sequence annotation: Help:Barcodes
sequence: https://synbiohub.org/public/igem/BBa_C0012_sequence/1

BBa_C0012
type: SequenceAnnotation
persistent identity: annotation7031
display id: annotation7031
version: 1
top level: lacI
location: https://synbiohub.org/public/igem/BBa_C0012/annotation7031/range7031/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_C0012/annotation7031/range7031/1
type: Range
persistent identity: range7031
display id: range7031
version: 1
top level: lacI
direction: direction/forward
start: 1
end: 1128
orientation: inline

lacI-LVA
type: SequenceAnnotation
persistent identity: annotation1723
display id: annotation1723
version: 1
top level: lacI
location: https://synbiohub.org/public/igem/BBa_C0012/annotation1723/range1723/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_C0012/annotation1723/range1723/1
type: Range
persistent identity: range1723
display id: range1723
version: 1
top level: lacI
direction: direction/forward
start: 1
end: 1128
orientation: inline

LVA
type: SequenceAnnotation
persistent identity: annotation1722
display id: annotation1722
version: 1
top level: lacI
location: https://synbiohub.org/public/igem/BBa_C0012/annotation1722/range1722/1
role: feature/tag
role: SO:0000324

https://synbiohub.org/public/igem/BBa_C0012/annotation1722/range1722/1
type: Range
persistent identity: range1722
display id: range1722
version: 1
top level: lacI
direction: direction/forward
start: 1090
end: 1128
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2213988
display id: annotation2213988
version: 1
top level: lacI
location: https://synbiohub.org/public/igem/BBa_C0012/annotation2213988/range2213988/1
role: feature/barcode
role: SO:0000807

https://synbiohub.org/public/igem/BBa_C0012/annotation2213988/range2213988/1
type: Range
persistent identity: range2213988
display id: range2213988
version: 1
top level: lacI
direction: direction/forward
start: 1129
end: 1153
orientation: inline

lacI+pL
type: ComponentDefinition
persistent identity: BBa R0011
display id: BBa_R0011
version: 1
was generated by: iGEM to SBOL conversion
description: Promoter (lacI regulated, lambda pL hybrid)
created: 2003-01-31T12:00:00Z
modified: 2015-05-08T01:14:14Z
mutable provenance: represillator of Elowitz and Leibler (2000)
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Inverting regulatory region controlled by LacI (<bb_part>BBa_C0010</bb_part>, <bb_part>BBa_C0011</bb_part>, etc.) The PLlac 0-1 promoter is a hybrid regulatory region consisting of the promoter P(L) of phage lambda with the cI binding sites replaced with lacO1. The hybrid design allows for strong promotion that can nevertheless be tightly repressed by LacI, the Lac inhibitor (i.e. repressor) (<bb_part>BBa_C0010</bb_part>) ([LUTZ97]). The activity of the promoter can be regulated over a >600-fold range by IPTG in E.Coli DH5-alpha-Z1 (same paper reference).
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: hybrid promoter design to create strong promoter that is, at the same time, highly repressible. note that the upstream operator installed in this hybrid is slightly different than the one in the original source (Lutz and Bujard, 1997). the most upstream operator region is slightly truncated in the represillator version, so that both operators in the hybrid are the same sequence. see references for details. also, the sequence has been truncated after the transcriptional start site. LacI binds to this regulator. This part is incompatible with species containing active LacI coding regions. Lactose and IPTG disable the operation of LacI and increase transcription. This part is incompatible with environments containing lactose or lactose analogs.
star: true
creator: Neelaksh Varshney, Grace Kenney, Daniel Shen, Samantha Sutton
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: BBa_R0011
sequence annotation: lac O1
sequence annotation: -35
sequence annotation: -10
sequence: https://synbiohub.org/public/igem/BBa_R0011_sequence/1

BBa_R0011
type: SequenceAnnotation
persistent identity: annotation7064
display id: annotation7064
version: 1
top level: lacI+pL
location: https://synbiohub.org/public/igem/BBa_R0011/annotation7064/range7064/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_R0011/annotation7064/range7064/1
type: Range
persistent identity: range7064
display id: range7064
version: 1
top level: lacI+pL
direction: direction/forward
start: 1
end: 54
orientation: inline

lac O1
type: SequenceAnnotation
persistent identity: annotation1999
display id: annotation1999
version: 1
top level: lacI+pL
location: https://synbiohub.org/public/igem/BBa_R0011/annotation1999/range1999/1
role: SO:0000057
role: feature/operator

https://synbiohub.org/public/igem/BBa_R0011/annotation1999/range1999/1
type: Range
persistent identity: range1999
display id: range1999
version: 1
top level: lacI+pL
direction: direction/forward
start: 3
end: 19
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation2000
display id: annotation2000
version: 1
top level: lacI+pL
location: https://synbiohub.org/public/igem/BBa_R0011/annotation2000/range2000/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_R0011/annotation2000/range2000/1
type: Range
persistent identity: range2000
display id: range2000
version: 1
top level: lacI+pL
direction: direction/forward
start: 20
end: 25
orientation: inline

lac O1
type: SequenceAnnotation
persistent identity: annotation2001
display id: annotation2001
version: 1
top level: lacI+pL
location: https://synbiohub.org/public/igem/BBa_R0011/annotation2001/range2001/1
role: SO:0000057
role: feature/operator

https://synbiohub.org/public/igem/BBa_R0011/annotation2001/range2001/1
type: Range
persistent identity: range2001
display id: range2001
version: 1
top level: lacI+pL
direction: direction/forward
start: 26
end: 42
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation2002
display id: annotation2002
version: 1
top level: lacI+pL
location: https://synbiohub.org/public/igem/BBa_R0011/annotation2002/range2002/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_R0011/annotation2002/range2002/1
type: Range
persistent identity: range2002
display id: range2002
version: 1
top level: lacI+pL
direction: direction/forward
start: 43
end: 48
orientation: inline

https://synbiohub.org/public/igem/BBa_J05050_sequence/1
type: Sequence
persistent identity: BBa J05050 sequence
display id: BBa_J05050_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagaaagaggagaaatactagatggtgaatgtgaaaccagtaacgttatacgatgtcgcagagtatgccggtgtctcttatcagaccgtttcccgcgtggtgaaccaggccagccacgtttctgcgaaaacgcgggaaaaagtggaagcggcgatggcggagctgaattacattcccaaccgcgtggcacaacaactggcgggcaaacagtcgttgctgattggcgttgccacctccagtctggccctgcacgcgccgtcgcaaattgtcgcggcgattaaatctcgcgccgatcaactgggtgccagcgtggtggtgtcgatggtagaacgaagcggcgtcgaagcctgtaaagcggcggtgcacaatcttctcgcgcaacgcgtcagtgggctgatcattaactatccgctggatgaccaggatgccattgctgtggaagctgcctgcactaatgttccggcgttatttcttgatgtctctgaccagacacccatcaacagtattattttctcccatgaagacggtacgcgactgggcgtggagcatctggtcgcattgggtcaccagcaaatcgcgctgttagcgggcccattaagttctgtctcggcgcgtctgcgtctggctggctggcataaatatctcactcgcaatcaaattcagccgatagcggaacgggaaggcgactggagtgccatgtccggttttcaacaaaccatgcaaatgctgaatgagggcatcgttcccactgcgatgctggttgccaacgatcagatggcgctgggcgcaatgcgcgccattaccgagtccgggctgcgcgttggtgcggatatctcggtagtgggatacgacgataccgaagacagctcatgttatatcccgccgttaaccaccatcaaacaggattttcgcctgctggggcaaaccagcgtggaccgcttgctgcaactctctcagggccaggcggtgaagggcaatcagctgttgcccgtctcactggtgaaaagaaaaaccaccctggcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcaggctgcaaacgacgaaaactacgctttagtagcttaataactctgatagtgctagtgtagatctctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagaattgtgagcggataacaattgacattgtgagcggataacaagatactgagcacatactagagaaagaggagaaatactagatgagcacaaaaaagaaaccattaacacaagagcagcttgaggacgcacgtcgccttaaagcaatttatgaaaaaaagaaaaatgaacttggcttatcccaggaatctgtcgcagacaagatggggatggggcagtcaggcgttggtgctttatttaatggcatcaatgcattaaatgcttataacgccgcattgcttgcaaaaattctcaaagttagcgttgaagaatttagcccttcaatcgccagagaaatctacgagatgtatgaagcggttagtatgcagccgtcacttagaagtgagtatgagtaccctgttttttctcatgttcaggcagggatgttctcacctgagcttagaacctttaccaaaggtgatgcggagagatgggtaagcacaaccaaaaaagccagtgattctgcattctggcttgaggttgaaggtaattccatgaccgcaccaacaggctccaagccaagctttcctgacggaatgttaattctcgttgaccctgagcaggctgttgagccaggtgatttct…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J05040_sequence/1
type: Sequence
persistent identity: BBa J05040 sequence
display id: BBa_J05040_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gcaaccattatcaccgccagaggtaaaatagtcaacacgcacggtgttagatatttataaatagtggtgatagatttaacgttactagagcctcgcccccgggggcgagggccccgcctccggaggcggggtactagagaaagaggagaaatactagatgctggaaccgggcgaaaaaccgtataaatgcccggaatgcggcaaaagctttagcgatccgggcaacctggtgcgccatcagcgcacccataccggcgaaaaaccgtataaatgcccggaatgcggcaaaagctttagccgcagcgataaactggtgcgccatcagcgcacccataccggcgaaaaaccgtataaatgcccggaatgcggcaaaagctttagccagcgcgcgcatctggaacgccatcagcgcacccataccggcaaaaaaaccagcgaatatattgatggcctggaaacccgcatgagcgcgtgcaccgcgcagaaccaggaactgcaacgcaaagtgctgcatctggaaaaacagaacctgagcctgctggaacagctgaaaaaactgcaagcgattgtggtgcagagcaccagctaataacgctgatagtgctagtgtagatcgctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagtaacaccgtgcgtgttgactattttacctctggcggtgataatggttgctactagagcctcgcccccgggggcgagggctccggccccggggccggagtactagagaaagaggagaaatactagatgctggaaccgggcgaaaaaccgtataaatgcccggaatgcggcaaaagctttagccgcagcgataaactggtgcgccatcagcgcacccataccggcgaaaaaccgtataaatgcccggaatgcggcaaaagctttagcgatccgggccatctggtgcgccatcagcgcacccataccggcgaaaaaccgtataaatgcccggaatgcggcaaaagctttagccagcgcgcgcatctggaacgccatcagcgcacccataccggcaaaaaaaccagcacccgcattcaggtgctggaagaaaaagtggaaatgctgcataacctggtggatgattggcagcgcaaatataaactgctggaaagcgaatttagcgataccaaagaaaacctgcaaaaaagcattgcgctgaacaacgaactgcaaaaagcgctgtaataactctgatagtgctagtgtagatctctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagaggcaaccattatcaccgccagaggtaaaatagtcaacacgcacggtgttagatatttataaatagtggtgatagatttaacgttactagagcctcgcccccgggggcgagggctccggccccggggccggagtactagagaaagaggagaaatactagatgctggaaccgggcgaaaaaccgtataaatgcccggaatgcggcaaaagctttagccgcagcgataaactggtgcgccatcagcgcacccataccggcgaaaaaccgtataaatgcccggaatgcggcaaaagctttagcgatccgggccatctggtgcgccatcagcgcacccataccggcgaaaaaccgtataaatgcccggaatgcggcaaaagctttagccagcgcgcgcatctggaacgccatcagcgcacccataccggcaaaaaaaccagcacccgcattcaggtgctggaagaaaaagtggaaatgctgcataacctggtggatgattggcagcgca…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J05500_sequence/1
type: Sequence
persistent identity: BBa J05500 sequence
display id: BBa_J05500_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagaaagaggagaaatactagatggtgaatgtgaaaccagtaacgttatacgatgtcgcagagtatgccggtgtctcttatcagaccgtttcccgcgtggtgaaccaggccagccacgtttctgcgaaaacgcgggaaaaagtggaagcggcgatggcggagctgaattacattcccaaccgcgtggcacaacaactggcgggcaaacagtcgttgctgattggcgttgccacctccagtctggccctgcacgcgccgtcgcaaattgtcgcggcgattaaatctcgcgccgatcaactgggtgccagcgtggtggtgtcgatggtagaacgaagcggcgtcgaagcctgtaaagcggcggtgcacaatcttctcgcgcaacgcgtcagtgggctgatcattaactatccgctggatgaccaggatgccattgctgtggaagctgcctgcactaatgttccggcgttatttcttgatgtctctgaccagacacccatcaacagtattattttctcccatgaagacggtacgcgactgggcgtggagcatctggtcgcattgggtcaccagcaaatcgcgctgttagcgggcccattaagttctgtctcggcgcgtctgcgtctggctggctggcataaatatctcactcgcaatcaaattcagccgatagcggaacgggaaggcgactggagtgccatgtccggttttcaacaaaccatgcaaatgctgaatgagggcatcgttcccactgcgatgctggttgccaacgatcagatggcgctgggcgcaatgcgcgccattaccgagtccgggctgcgcgttggtgcggatatctcggtagtgggatacgacgataccgaagacagctcatgttatatcccgccgttaaccaccatcaaacaggattttcgcctgctggggcaaaccagcgtggaccgcttgctgcaactctctcagggccaggcggtgaagggcaatcagctgttgcccgtctcactggtgaaaagaaaaaccaccctggcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcaggctgcaaacgacgaaaactacgctttagtagcttaataactctgatagtgctagtgtagatctctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagaattgtgagcggataacaattgacattgtgagcggataacaagatactgagcacatactagagaaagaggagaaatactagatgagcacaaaaaagaaaccattaacacaagagcagcttgaggacgcacgtcgccttaaagcaatttatgaaaaaaagaaaaatgaacttggcttatcccaggaatctgtcgcagacaagatggggatggggcagtcaggcgttggtgctttatttaatggcatcaatgcattaaatgcttataacgccgcattgcttgcaaaaattctcaaagttagcgttgaagaatttagcccttcaatcgccagagaaatctacgagatgtatgaagcggttagtatgcagccgtcacttagaagtgagtatgagtaccctgttttttctcatgttcaggcagggatgttctcacctgagcttagaacctttaccaaaggtgatgcggagagatgggtaagcacaaccaaaaaagccagtgattctgcattctggcttgaggttgaaggtaattccatgaccgcaccaacaggctccaagccaagctttcctgacggaatgttaattctcgttgaccctgagcaggctgttgagccaggtgatttct…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J05108_sequence/1
type: Sequence
persistent identity: BBa J05108 sequence
display id: BBa_J05108_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgctggaaccgggcgaaaaaccgtataaatgcccggaatgcggcaaaagctttagcgatccgggcaacctggtgcgccatcagcgcacccataccggcgaaaaaccgtataaatgcccggaatgcggcaaaagctttagccgcagcgataaactggtgcgccatcagcgcacccataccggcgaaaaaccgtataaatgcccggaatgcggcaaaagctttagccagcgcgcgcatctggaacgccatcagcgcacccataccggcaaaaaaaccagcgaatatattgatggcctggaaacccgcatgagcgcgtgcaccgcgcagaaccaggaactgcaacgcaaagtgctgcatctggaaaaacagaacctgagcctgctggaacagctgaaaaaactgcaagcgattgtggtgcagagcaccagctaataacgctgatagtgctagtgtagatcgc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J05215_sequence/1
type: Sequence
persistent identity: BBa J05215 sequence
display id: BBa_J05215_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: myers
owned by: james
elements: cctcgcccccgggggcgagggccccgcctccggaggcgggg
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0034_sequence/1
type: Sequence
persistent identity: BBa B0034 sequence
display id: BBa_B0034_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J05110_sequence/1
type: Sequence
persistent identity: BBa J05110 sequence
display id: BBa_J05110_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgctggaaccgggcgaaaaaccgtataaatgcccggaatgcggcaaaagctttagccgcagcgataaactggtgcgccatcagcgcacccataccggcgaaaaaccgtataaatgcccggaatgcggcaaaagctttagcgatccgggccatctggtgcgccatcagcgcacccataccggcgaaaaaccgtataaatgcccggaatgcggcaaaagctttagccagcgcgcgcatctggaacgccatcagcgcacccataccggcaaaaaaaccagcacccgcattcaggtgctggaagaaaaagtggaaatgctgcataacctggtggatgattggcagcgcaaatataaactgctggaaagcgaatttagcgataccaaagaaaacctgcaaaaaagcattgcgctgaacaacgaactgcaaaaagcgctgtaataactctgatagtgctagtgtagatctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I12007_sequence/1
type: Sequence
persistent identity: BBa I12007 sequence
display id: BBa_I12007_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gcaaccattatcaccgccagaggtaaaatagtcaacacgcacggtgttagatatttataaatagtggtgatagatttaacgt
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J05217_sequence/1
type: Sequence
persistent identity: BBa J05217 sequence
display id: BBa_J05217_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: cctcgcccccgggggcgagggctccggccccggggccggag
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J05218_sequence/1
type: Sequence
persistent identity: BBa J05218 sequence
display id: BBa_J05218_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: cgtcccctccggaggggacggccccgcctccggaggcgggg
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_R0051_sequence/1
type: Sequence
persistent identity: BBa R0051 sequence
display id: BBa_R0051_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: taacaccgtgcgtgttgactattttacctctggcggtgataatggttgc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0030_sequence/1
type: Sequence
persistent identity: BBa B0030 sequence
display id: BBa_B0030_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: attaaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_E0040_sequence/1
type: Sequence
persistent identity: BBa E0040 sequence
display id: BBa_E0040_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: myers
owned by: james
elements: atgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J05111_sequence/1
type: Sequence
persistent identity: BBa J05111 sequence
display id: BBa_J05111_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgctggaaccgggcgaaaaaccgtataaatgcccggaatgcggcaaaagctttagccagcgcgcgcatctggaacgccatcagcgcacccataccggcgaaaaaccgtataaatgcccggaatgcggcaaaagctttagcgattgccgcgatctggcgcgccatcagcgcacccataccggcgaaaaaccgtataaatgcccggaatgcggcaaaagctttagccgcagcgataaactggtgcgccatcagcgcacccataccggcaaaaaaaccagccagcagcgccatgtgctggaaagcgaaaaaaaccagctgctgcaacaggtggatcatctgaaacaggaaattagccgcctggtgcgcgaacgcgatgcgtataaagaaaaatatgaaaaactggtgagcagcggctttcgcgaaaactaataacactgatagtgctagtgtagatcac
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_C0051_sequence/1
type: Sequence
persistent identity: BBa C0051 sequence
display id: BBa_C0051_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgagcacaaaaaagaaaccattaacacaagagcagcttgaggacgcacgtcgccttaaagcaatttatgaaaaaaagaaaaatgaacttggcttatcccaggaatctgtcgcagacaagatggggatggggcagtcaggcgttggtgctttatttaatggcatcaatgcattaaatgcttataacgccgcattgcttgcaaaaattctcaaagttagcgttgaagaatttagcccttcaatcgccagagaaatctacgagatgtatgaagcggttagtatgcagccgtcacttagaagtgagtatgagtaccctgttttttctcatgttcaggcagggatgttctcacctgagcttagaacctttaccaaaggtgatgcggagagatgggtaagcacaaccaaaaaagccagtgattctgcattctggcttgaggttgaaggtaattccatgaccgcaccaacaggctccaagccaagctttcctgacggaatgttaattctcgttgaccctgagcaggctgttgagccaggtgatttctgcatagccagacttgggggtgatgagtttaccttcaagaaactgatcagggatagcggtcaggtgtttttacaaccactaaacccacagtacccaatgatcccatgcaatgagagttgttccgttgtggggaaagttatcgctagtcagtggcctgaagagacgtttggcgctgcaaacgacgaaaactacgctttagtagcttaataacgctgatagtgctagtgtagatcgc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_R0040_sequence/1
type: Sequence
persistent identity: BBa R0040 sequence
display id: BBa_R0040_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcac
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_C0012_sequence/1
type: Sequence
persistent identity: BBa C0012 sequence
display id: BBa_C0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggtgaatgtgaaaccagtaacgttatacgatgtcgcagagtatgccggtgtctcttatcagaccgtttcccgcgtggtgaaccaggccagccacgtttctgcgaaaacgcgggaaaaagtggaagcggcgatggcggagctgaattacattcccaaccgcgtggcacaacaactggcgggcaaacagtcgttgctgattggcgttgccacctccagtctggccctgcacgcgccgtcgcaaattgtcgcggcgattaaatctcgcgccgatcaactgggtgccagcgtggtggtgtcgatggtagaacgaagcggcgtcgaagcctgtaaagcggcggtgcacaatcttctcgcgcaacgcgtcagtgggctgatcattaactatccgctggatgaccaggatgccattgctgtggaagctgcctgcactaatgttccggcgttatttcttgatgtctctgaccagacacccatcaacagtattattttctcccatgaagacggtacgcgactgggcgtggagcatctggtcgcattgggtcaccagcaaatcgcgctgttagcgggcccattaagttctgtctcggcgcgtctgcgtctggctggctggcataaatatctcactcgcaatcaaattcagccgatagcggaacgggaaggcgactggagtgccatgtccggttttcaacaaaccatgcaaatgctgaatgagggcatcgttcccactgcgatgctggttgccaacgatcagatggcgctgggcgcaatgcgcgccattaccgagtccgggctgcgcgttggtgcggatatctcggtagtgggatacgacgataccgaagacagctcatgttatatcccgccgttaaccaccatcaaacaggattttcgcctgctggggcaaaccagcgtggaccgcttgctgcaactctctcagggccaggcggtgaagggcaatcagctgttgcccgtctcactggtgaaaagaaaaaccaccctggcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcaggctgcaaacgacgaaaactacgctttagtagcttaataactctgatagtgctagtgtagatctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_R0011_sequence/1
type: Sequence
persistent identity: BBa R0011 sequence
display id: BBa_R0011_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aattgtgagcggataacaattgacattgtgagcggataacaagatactgagcaca
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: Chris J. Myers
creator: James Alastair McLaughlin
ended at time: 2017-03-06T15:00:00.000Z