LOCUS       BBa_J05306              1270 bp    DNA     linear       02-Jan-2025
DEFINITION  R3 sys A + Reporter
ACCESSION   BBa_J05306
VERSION     BBa_J05306.1  
FEATURES             Location/Qualifiers
     promoter        1..24
                     /label=BBa_J05213
     misc_feature    37..40
                     /label=conserved
     RBS             33..44
                     /label=BBa_B0034
     CDS             84..167
                     /label=Finger 1
     CDS             168..251
                     /label=Finger 2
     CDS             252..335
                     /label=Finger 3
     misc_feature    117..140
                     /label=ZF-GAG
     misc_feature    201..224
                     /label=ZF-GGC
     misc_feature    285..308
                     /label=ZF-GGG
     misc_feature    360..384
                     /label=Help:Barcodes
     CDS             51..359
                     /label=BBa_J05101
     RBS             393..407
                     /label=RBS-1\Strong
     RBS             400..404
                     /label=RBS
     misc_feature    393..407
                     /label=BBa_B0030
     RBS             393..407
                     /label=BBa_B0030
     CDS             414..1133
                     /label=GFP protein
     CDS             414..1133
                     /label=BBa_E0040
     stem_loop       1153..1196
                     /label=stem_loop
     misc_feature    1142..1221
                     /label=BBa_B0010
     terminator      1142..1221
                     /label=BBa_B0010
     stem_loop       1237..1256
                     /label=T7 TE
     misc_feature    1263..1263
                     /label=stop
     polyA_site      1257..1270
                     /label=polya
     misc_feature    1230..1270
                     /label=BBa_B0012
     terminator      1230..1270
                     /label=BBa_B0012
ORIGIN
        1 ggaggggacg cgcgggggcc ggagtactag agaaagagga gaaatactag atggcgcagg
       61 cggcgctgga accgaaagaa aaaccgtatg cgtgcccgga atgcggcaaa agctttagcc
      121 gcagcgataa cctggtgcgc catcagcgca cccataccgg cgaaaaaccg tataaatgcc
      181 cggaatgcgg caaaagcttt agcgatccgg gccatctggt gcgccatcag cgcacccata
      241 ccggcgaaaa accgtataaa tgcccggaat gcggcaaaag ctttagccgc agcgataaac
      301 tggtgcgcca tcagcgcacc cataccggca aaaaaaccag cggccaggcg ggctaataac
      361 tctgatagtg ctagtgtaga tctctactag agattaaaga ggagaaatac tagatgcgta
      421 aaggagaaga acttttcact ggagttgtcc caattcttgt tgaattagat ggtgatgtta
      481 atgggcacaa attttctgtc agtggagagg gtgaaggtga tgcaacatac ggaaaactta
      541 cccttaaatt tatttgcact actggaaaac tacctgttcc atggccaaca cttgtcacta
      601 ctttcggtta tggtgttcaa tgctttgcga gatacccaga tcatatgaaa cagcatgact
      661 ttttcaagag tgccatgccc gaaggttatg tacaggaaag aactatattt ttcaaagatg
      721 acgggaacta caagacacgt gctgaagtca agtttgaagg tgataccctt gttaatagaa
      781 tcgagttaaa aggtattgat tttaaagaag atggaaacat tcttggacac aaattggaat
      841 acaactataa ctcacacaat gtatacatca tggcagacaa acaaaagaat ggaatcaaag
      901 ttaacttcaa aattagacac aacattgaag atggaagcgt tcaactagca gaccattatc
      961 aacaaaatac tccaattggc gatggccctg tccttttacc agacaaccat tacctgtcca
     1021 cacaatctgc cctttcgaaa gatcccaacg aaaagagaga ccacatggtc cttcttgagt
     1081 ttgtaacagc tgctgggatt acacatggca tggatgaact atacaaataa taatactaga
     1141 gccaggcatc aaataaaacg aaaggctcag tcgaaagact gggcctttcg ttttatctgt
     1201 tgtttgtcgg tgaacgctct ctactagagt cacactggct caccttcggg tgggcctttc
     1261 tgcgtttata
//