LOCUS       BBa_J05313               670 bp    DNA     linear       21-Dec-2024
DEFINITION  R2 state
ACCESSION   BBa_J05313
VERSION     BBa_J05313.1  
FEATURES             Location/Qualifiers
     promoter        1..41
                     /label=BBa_J05217
     misc_feature    54..57
                     /label=conserved
     RBS             50..61
                     /label=BBa_B0034
     CDS             89..172
                     /label=Finger 1
     misc_feature    122..145
                     /label=ZF-GGG
     CDS             173..256
                     /label=Finger 2
     misc_feature    206..229
                     /label=ZF-GGC
     CDS             257..340
                     /label=Finger 3
     misc_feature    290..313
                     /label=ZF-GGA
     misc_feature    509..533
                     /label=Help:Barcodes
     CDS             68..533
                     /label=BBa_J05110
     stem_loop       553..596
                     /label=stem_loop
     misc_feature    542..621
                     /label=BBa_B0010
     terminator      542..621
                     /label=BBa_B0010
     stem_loop       637..656
                     /label=T7 TE
     misc_feature    663..663
                     /label=stop
     polyA_site      657..670
                     /label=polya
     misc_feature    630..670
                     /label=BBa_B0012
     terminator      630..670
                     /label=BBa_B0012
     terminator      542..670
                     /label=BBa_B0015
ORIGIN
        1 cctcgccccc gggggcgagg gctccggccc cggggccgga gtactagaga aagaggagaa
       61 atactagatg ctggaaccgg gcgaaaaacc gtataaatgc ccggaatgcg gcaaaagctt
      121 tagccgcagc gataaactgg tgcgccatca gcgcacccat accggcgaaa aaccgtataa
      181 atgcccggaa tgcggcaaaa gctttagcga tccgggccat ctggtgcgcc atcagcgcac
      241 ccataccggc gaaaaaccgt ataaatgccc ggaatgcggc aaaagcttta gccagcgcgc
      301 gcatctggaa cgccatcagc gcacccatac cggcaaaaaa accagcaccc gcattcaggt
      361 gctggaagaa aaagtggaaa tgctgcataa cctggtggat gattggcagc gcaaatataa
      421 actgctggaa agcgaattta gcgataccaa agaaaacctg caaaaaagca ttgcgctgaa
      481 caacgaactg caaaaagcgc tgtaataact ctgatagtgc tagtgtagat ctctactaga
      541 gccaggcatc aaataaaacg aaaggctcag tcgaaagact gggcctttcg ttttatctgt
      601 tgtttgtcgg tgaacgctct ctactagagt cacactggct caccttcggg tgggcctttc
      661 tgcgtttata
//