LOCUS       BBa_J05316              1419 bp    DNA     linear       21-Dec-2024
DEFINITION  R3 state + Reporter
ACCESSION   BBa_J05316
VERSION     BBa_J05316.1  
FEATURES             Location/Qualifiers
     promoter        1..41
                     /label=BBa_J05217
     misc_feature    54..57
                     /label=conserved
     RBS             50..61
                     /label=BBa_B0034
     CDS             89..172
                     /label=Finger 1
     misc_feature    122..145
                     /label=ZF-GGG
     CDS             173..256
                     /label=Finger 2
     misc_feature    206..229
                     /label=ZF-GGC
     CDS             257..340
                     /label=Finger 3
     misc_feature    290..313
                     /label=ZF-GGA
     misc_feature    509..533
                     /label=Help:Barcodes
     CDS             68..533
                     /label=BBa_J05110
     RBS             542..556
                     /label=RBS-1\Strong
     RBS             549..553
                     /label=RBS
     misc_feature    542..556
                     /label=BBa_B0030
     RBS             542..556
                     /label=BBa_B0030
     CDS             563..1282
                     /label=GFP protein
     CDS             563..1282
                     /label=BBa_E0040
     stem_loop       1302..1345
                     /label=stem_loop
     misc_feature    1291..1370
                     /label=BBa_B0010
     terminator      1291..1370
                     /label=BBa_B0010
     stem_loop       1386..1405
                     /label=T7 TE
     misc_feature    1412..1412
                     /label=stop
     polyA_site      1406..1419
                     /label=polya
     misc_feature    1379..1419
                     /label=BBa_B0012
     terminator      1379..1419
                     /label=BBa_B0012
     misc_feature    542..1419
                     /label=BBa_E0840
ORIGIN
        1 cctcgccccc gggggcgagg gctccggccc cggggccgga gtactagaga aagaggagaa
       61 atactagatg ctggaaccgg gcgaaaaacc gtataaatgc ccggaatgcg gcaaaagctt
      121 tagccgcagc gataaactgg tgcgccatca gcgcacccat accggcgaaa aaccgtataa
      181 atgcccggaa tgcggcaaaa gctttagcga tccgggccat ctggtgcgcc atcagcgcac
      241 ccataccggc gaaaaaccgt ataaatgccc ggaatgcggc aaaagcttta gccagcgcgc
      301 gcatctggaa cgccatcagc gcacccatac cggcaaaaaa accagcaccc gcattcaggt
      361 gctggaagaa aaagtggaaa tgctgcataa cctggtggat gattggcagc gcaaatataa
      421 actgctggaa agcgaattta gcgataccaa agaaaacctg caaaaaagca ttgcgctgaa
      481 caacgaactg caaaaagcgc tgtaataact ctgatagtgc tagtgtagat ctctactaga
      541 gattaaagag gagaaatact agatgcgtaa aggagaagaa cttttcactg gagttgtccc
      601 aattcttgtt gaattagatg gtgatgttaa tgggcacaaa ttttctgtca gtggagaggg
      661 tgaaggtgat gcaacatacg gaaaacttac ccttaaattt atttgcacta ctggaaaact
      721 acctgttcca tggccaacac ttgtcactac tttcggttat ggtgttcaat gctttgcgag
      781 atacccagat catatgaaac agcatgactt tttcaagagt gccatgcccg aaggttatgt
      841 acaggaaaga actatatttt tcaaagatga cgggaactac aagacacgtg ctgaagtcaa
      901 gtttgaaggt gatacccttg ttaatagaat cgagttaaaa ggtattgatt ttaaagaaga
      961 tggaaacatt cttggacaca aattggaata caactataac tcacacaatg tatacatcat
     1021 ggcagacaaa caaaagaatg gaatcaaagt taacttcaaa attagacaca acattgaaga
     1081 tggaagcgtt caactagcag accattatca acaaaatact ccaattggcg atggccctgt
     1141 ccttttacca gacaaccatt acctgtccac acaatctgcc ctttcgaaag atcccaacga
     1201 aaagagagac cacatggtcc ttcttgagtt tgtaacagct gctgggatta cacatggcat
     1261 ggatgaacta tacaaataat aatactagag ccaggcatca aataaaacga aaggctcagt
     1321 cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc tactagagtc
     1381 acactggctc accttcgggt gggcctttct gcgtttata
//