LOCUS BBa_J05503 4270 bp DNA linear 22-Nov-2024 DEFINITION Sensing Device A (cI) TESTER (Pr+GFP+Prm+RFP) ACCESSION BBa_J05503 VERSION BBa_J05503.1 FEATURES Location/Qualifiers misc_feature 1..19 /label=TetR 1 misc_feature 1..54 /label=BBa_R0040 promoter 20..25 /label=-35 misc_feature 26..44 /label=TetR 2 promoter 43..48 /label=-10 promoter 1..54 /label=BBa_R0040 misc_feature 67..70 /label=conserved RBS 63..74 /label=BBa_B0034 misc_feature 1170..1208 /label=LVA CDS 81..1208 /label=lacI-LVA misc_feature 81..1208 /label=BBa_C0012 misc_feature 1209..1233 /label=Help:Barcodes CDS 81..1208 /label=BBa_C0012 stem_loop 1253..1296 /label=stem_loop misc_feature 1242..1321 /label=BBa_B0010 terminator 1242..1321 /label=BBa_B0010 stem_loop 1337..1356 /label=T7 TE misc_feature 1363..1363 /label=stop polyA_site 1357..1370 /label=polya misc_feature 1330..1370 /label=BBa_B0012 terminator 1330..1370 /label=BBa_B0012 misc_feature 1381..1397 /label=lac O1 promoter 1398..1403 /label=-35 misc_feature 1404..1420 /label=lac O1 promoter 1421..1426 /label=-10 misc_feature 1379..1432 /label=BBa_R0011 promoter 1379..1432 /label=BBa_R0011 misc_feature 1446..1449 /label=conserved RBS 1442..1453 /label=BBa_B0034 CDS 1463..2170 /label=cI lambda misc_feature 2171..2203 /label=LVA misc_feature 2210..2234 /label=Help:Barcodes CDS 1460..2209 /label=BBa_C0051 stem_loop 2254..2297 /label=stem_loop misc_feature 2243..2322 /label=BBa_B0010 terminator 2243..2322 /label=BBa_B0010 stem_loop 2338..2357 /label=T7 TE misc_feature 2364..2364 /label=stop polyA_site 2358..2371 /label=polya misc_feature 2331..2371 /label=BBa_B0012 terminator 2331..2371 /label=BBa_B0012 promoter 2417..2422 /label=-10 promoter 2394..2399 /label=-35 misc_feature 2404..2420 /label=OR1 misc_feature 2380..2396 /label=OR2 misc_feature 2380..2428 /label=BBa_R0051 promoter 2380..2428 /label=BBa_R0051 misc_feature 2441..2444 /label=conserved RBS 2437..2448 /label=BBa_B0034 CDS 2455..3174 /label=GFP protein CDS 2455..3174 /label=BBa_E0040 stem_loop 3194..3237 /label=stem_loop misc_feature 3183..3262 /label=BBa_B0010 terminator 3183..3262 /label=BBa_B0010 stem_loop 3278..3297 /label=T7 TE misc_feature 3304..3304 /label=stop polyA_site 3298..3311 /label=polya misc_feature 3271..3311 /label=BBa_B0012 terminator 3271..3311 /label=BBa_B0012 misc_feature 3328..3344 /label=OR1 misc_feature 3352..3368 /label=OR2 promoter 3367..3372 /label=-35 misc_feature 3378..3384 /label=mutate to obliterate OR3 promoter 3390..3395 /label=-10 promoter 3320..3401 /label=BBa_I12007 misc_feature 3414..3417 /label=conserved RBS 3410..3421 /label=BBa_B0034 CDS 3428..4102 /label=mrfp1 misc_feature 4109..4133 /label=Help:Barcodes CDS 3428..4108 /label=BBa_E1010 stem_loop 4153..4196 /label=stem_loop misc_feature 4142..4221 /label=BBa_B0010 terminator 4142..4221 /label=BBa_B0010 stem_loop 4237..4256 /label=T7 TE misc_feature 4263..4263 /label=stop polyA_site 4257..4270 /label=polya misc_feature 4230..4270 /label=BBa_B0012 terminator 4230..4270 /label=BBa_B0012 ORIGIN 1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag 61 agaaagagga gaaatactag atggtgaatg tgaaaccagt aacgttatac gatgtcgcag 121 agtatgccgg tgtctcttat cagaccgttt cccgcgtggt gaaccaggcc agccacgttt 181 ctgcgaaaac gcgggaaaaa gtggaagcgg cgatggcgga gctgaattac attcccaacc 241 gcgtggcaca acaactggcg ggcaaacagt cgttgctgat tggcgttgcc acctccagtc 301 tggccctgca cgcgccgtcg caaattgtcg cggcgattaa atctcgcgcc gatcaactgg 361 gtgccagcgt ggtggtgtcg atggtagaac gaagcggcgt cgaagcctgt aaagcggcgg 421 tgcacaatct tctcgcgcaa cgcgtcagtg ggctgatcat taactatccg ctggatgacc 481 aggatgccat tgctgtggaa gctgcctgca ctaatgttcc ggcgttattt cttgatgtct 541 ctgaccagac acccatcaac agtattattt tctcccatga agacggtacg cgactgggcg 601 tggagcatct ggtcgcattg ggtcaccagc aaatcgcgct gttagcgggc ccattaagtt 661 ctgtctcggc gcgtctgcgt ctggctggct ggcataaata tctcactcgc aatcaaattc 721 agccgatagc ggaacgggaa ggcgactgga gtgccatgtc cggttttcaa caaaccatgc 781 aaatgctgaa tgagggcatc gttcccactg cgatgctggt tgccaacgat cagatggcgc 841 tgggcgcaat gcgcgccatt accgagtccg ggctgcgcgt tggtgcggat atctcggtag 901 tgggatacga cgataccgaa gacagctcat gttatatccc gccgttaacc accatcaaac 961 aggattttcg cctgctgggg caaaccagcg tggaccgctt gctgcaactc tctcagggcc 1021 aggcggtgaa gggcaatcag ctgttgcccg tctcactggt gaaaagaaaa accaccctgg 1081 cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac 1141 gacaggtttc ccgactggaa agcgggcagg ctgcaaacga cgaaaactac gctttagtag 1201 cttaataact ctgatagtgc tagtgtagat ctctactaga gccaggcatc aaataaaacg 1261 aaaggctcag tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct 1321 ctactagagt cacactggct caccttcggg tgggcctttc tgcgtttata tactagagaa 1381 ttgtgagcgg ataacaattg acattgtgag cggataacaa gatactgagc acatactaga 1441 gaaagaggag aaatactaga tgagcacaaa aaagaaacca ttaacacaag agcagcttga 1501 ggacgcacgt cgccttaaag caatttatga aaaaaagaaa aatgaacttg gcttatccca 1561 ggaatctgtc gcagacaaga tggggatggg gcagtcaggc gttggtgctt tatttaatgg 1621 catcaatgca ttaaatgctt ataacgccgc attgcttgca aaaattctca aagttagcgt 1681 tgaagaattt agcccttcaa tcgccagaga aatctacgag atgtatgaag cggttagtat 1741 gcagccgtca cttagaagtg agtatgagta ccctgttttt tctcatgttc aggcagggat 1801 gttctcacct gagcttagaa cctttaccaa aggtgatgcg gagagatggg taagcacaac 1861 caaaaaagcc agtgattctg cattctggct tgaggttgaa ggtaattcca tgaccgcacc 1921 aacaggctcc aagccaagct ttcctgacgg aatgttaatt ctcgttgacc ctgagcaggc 1981 tgttgagcca ggtgatttct gcatagccag acttgggggt gatgagttta ccttcaagaa 2041 actgatcagg gatagcggtc aggtgttttt acaaccacta aacccacagt acccaatgat 2101 cccatgcaat gagagttgtt ccgttgtggg gaaagttatc gctagtcagt ggcctgaaga 2161 gacgtttggc gctgcaaacg acgaaaacta cgctttagta gcttaataac gctgatagtg 2221 ctagtgtaga tcgctactag agccaggcat caaataaaac gaaaggctca gtcgaaagac 2281 tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc tctactagag tcacactggc 2341 tcaccttcgg gtgggccttt ctgcgtttat atactagagt aacaccgtgc gtgttgacta 2401 ttttacctct ggcggtgata atggttgcta ctagagaaag aggagaaata ctagatgcgt 2461 aaaggagaag aacttttcac tggagttgtc ccaattcttg ttgaattaga tggtgatgtt 2521 aatgggcaca aattttctgt cagtggagag ggtgaaggtg atgcaacata cggaaaactt 2581 acccttaaat ttatttgcac tactggaaaa ctacctgttc catggccaac acttgtcact 2641 actttcggtt atggtgttca atgctttgcg agatacccag atcatatgaa acagcatgac 2701 tttttcaaga gtgccatgcc cgaaggttat gtacaggaaa gaactatatt tttcaaagat 2761 gacgggaact acaagacacg tgctgaagtc aagtttgaag gtgataccct tgttaataga 2821 atcgagttaa aaggtattga ttttaaagaa gatggaaaca ttcttggaca caaattggaa 2881 tacaactata actcacacaa tgtatacatc atggcagaca aacaaaagaa tggaatcaaa 2941 gttaacttca aaattagaca caacattgaa gatggaagcg ttcaactagc agaccattat 3001 caacaaaata ctccaattgg cgatggccct gtccttttac cagacaacca ttacctgtcc 3061 acacaatctg ccctttcgaa agatcccaac gaaaagagag accacatggt ccttcttgag 3121 tttgtaacag ctgctgggat tacacatggc atggatgaac tatacaaata ataatactag 3181 agccaggcat caaataaaac gaaaggctca gtcgaaagac tgggcctttc gttttatctg 3241 ttgtttgtcg gtgaacgctc tctactagag tcacactggc tcaccttcgg gtgggccttt 3301 ctgcgtttat atactagagg caaccattat caccgccaga ggtaaaatag tcaacacgca 3361 cggtgttaga tatttataaa tagtggtgat agatttaacg ttactagaga aagaggagaa 3421 atactagatg gcttcctccg aagacgttat caaagagttc atgcgtttca aagttcgtat 3481 ggaaggttcc gttaacggtc acgagttcga aatcgaaggt gaaggtgaag gtcgtccgta 3541 cgaaggtacc cagaccgcta aactgaaagt taccaaaggt ggtccgctgc cgttcgcttg 3601 ggacatcctg tccccgcagt tccagtacgg ttccaaagct tacgttaaac acccggctga 3661 catcccggac tacctgaaac tgtccttccc ggaaggtttc aaatgggaac gtgttatgaa 3721 cttcgaagac ggtggtgttg ttaccgttac ccaggactcc tccctgcaag acggtgagtt 3781 catctacaaa gttaaactgc gtggtaccaa cttcccgtcc gacggtccgg ttatgcagaa 3841 aaaaaccatg ggttgggaag cttccaccga acgtatgtac ccggaagacg gtgctctgaa 3901 aggtgaaatc aaaatgcgtc tgaaactgaa agacggtggt cactacgacg ctgaagttaa 3961 aaccacctac atggctaaaa aaccggttca gctgccgggt gcttacaaaa ccgacatcaa 4021 actggacatc acctcccaca acgaagacta caccatcgtt gaacagtacg aacgtgctga 4081 aggtcgtcac tccaccggtg cttaataacg ctgatagtgc tagtgtagat cgctactaga 4141 gccaggcatc aaataaaacg aaaggctcag tcgaaagact gggcctttcg ttttatctgt 4201 tgtttgtcgg tgaacgctct ctactagagt cacactggct caccttcggg tgggcctttc 4261 tgcgtttata //