LOCUS BBa_J05504 4236 bp DNA linear 26-Dec-2024 DEFINITION Sensing Device B (cI+LVA) TESTER (Pr+GFP+Prm+RFP) ACCESSION BBa_J05504 VERSION BBa_J05504.1 FEATURES Location/Qualifiers misc_feature 1..19 /label=TetR 1 misc_feature 1..54 /label=BBa_R0040 promoter 20..25 /label=-35 misc_feature 26..44 /label=TetR 2 promoter 43..48 /label=-10 promoter 1..54 /label=BBa_R0040 misc_feature 67..70 /label=conserved RBS 63..74 /label=BBa_B0034 misc_feature 1170..1208 /label=LVA CDS 81..1208 /label=lacI-LVA misc_feature 81..1208 /label=BBa_C0012 misc_feature 1209..1233 /label=Help:Barcodes CDS 81..1208 /label=BBa_C0012 stem_loop 1253..1296 /label=stem_loop misc_feature 1242..1321 /label=BBa_B0010 terminator 1242..1321 /label=BBa_B0010 stem_loop 1337..1356 /label=T7 TE misc_feature 1363..1363 /label=stop polyA_site 1357..1370 /label=polya misc_feature 1330..1370 /label=BBa_B0012 terminator 1330..1370 /label=BBa_B0012 misc_feature 1381..1397 /label=lac O1 promoter 1398..1403 /label=-35 misc_feature 1404..1420 /label=lac O1 promoter 1421..1426 /label=-10 misc_feature 1379..1432 /label=BBa_R0011 promoter 1379..1432 /label=BBa_R0011 misc_feature 1446..1449 /label=conserved RBS 1442..1453 /label=BBa_B0034 CDS 1463..2170 /label=cI lambda misc_feature 2171..2203 /label=LVA misc_feature 2210..2234 /label=Help:Barcodes CDS 1460..2209 /label=BBa_C0051 stem_loop 2250..2269 /label=T7 TE misc_feature 2276..2276 /label=stop polyA_site 2270..2283 /label=polya misc_feature 2243..2283 /label=BBa_B0012 terminator 2243..2283 /label=BBa_B0012 stem_loop 2304..2326 /label=stem_loop misc_feature 2292..2337 /label=BBa_B0011 terminator 2292..2337 /label=BBa_B0011 promoter 2383..2388 /label=-10 promoter 2360..2365 /label=-35 misc_feature 2370..2386 /label=OR1 misc_feature 2346..2362 /label=OR2 misc_feature 2346..2394 /label=BBa_R0051 promoter 2346..2394 /label=BBa_R0051 misc_feature 2407..2410 /label=conserved RBS 2403..2414 /label=BBa_B0034 CDS 2421..3140 /label=GFP protein CDS 2421..3140 /label=BBa_E0040 stem_loop 3160..3203 /label=stem_loop misc_feature 3149..3228 /label=BBa_B0010 terminator 3149..3228 /label=BBa_B0010 stem_loop 3244..3263 /label=T7 TE misc_feature 3270..3270 /label=stop polyA_site 3264..3277 /label=polya misc_feature 3237..3277 /label=BBa_B0012 terminator 3237..3277 /label=BBa_B0012 misc_feature 3294..3310 /label=OR1 misc_feature 3318..3334 /label=OR2 promoter 3333..3338 /label=-35 misc_feature 3344..3350 /label=mutate to obliterate OR3 promoter 3356..3361 /label=-10 promoter 3286..3367 /label=BBa_I12007 misc_feature 3380..3383 /label=conserved RBS 3376..3387 /label=BBa_B0034 CDS 3394..4068 /label=mrfp1 misc_feature 4075..4099 /label=Help:Barcodes CDS 3394..4074 /label=BBa_E1010 stem_loop 4119..4162 /label=stem_loop misc_feature 4108..4187 /label=BBa_B0010 terminator 4108..4187 /label=BBa_B0010 stem_loop 4203..4222 /label=T7 TE misc_feature 4229..4229 /label=stop polyA_site 4223..4236 /label=polya misc_feature 4196..4236 /label=BBa_B0012 terminator 4196..4236 /label=BBa_B0012 ORIGIN 1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag 61 agaaagagga gaaatactag atggtgaatg tgaaaccagt aacgttatac gatgtcgcag 121 agtatgccgg tgtctcttat cagaccgttt cccgcgtggt gaaccaggcc agccacgttt 181 ctgcgaaaac gcgggaaaaa gtggaagcgg cgatggcgga gctgaattac attcccaacc 241 gcgtggcaca acaactggcg ggcaaacagt cgttgctgat tggcgttgcc acctccagtc 301 tggccctgca cgcgccgtcg caaattgtcg cggcgattaa atctcgcgcc gatcaactgg 361 gtgccagcgt ggtggtgtcg atggtagaac gaagcggcgt cgaagcctgt aaagcggcgg 421 tgcacaatct tctcgcgcaa cgcgtcagtg ggctgatcat taactatccg ctggatgacc 481 aggatgccat tgctgtggaa gctgcctgca ctaatgttcc ggcgttattt cttgatgtct 541 ctgaccagac acccatcaac agtattattt tctcccatga agacggtacg cgactgggcg 601 tggagcatct ggtcgcattg ggtcaccagc aaatcgcgct gttagcgggc ccattaagtt 661 ctgtctcggc gcgtctgcgt ctggctggct ggcataaata tctcactcgc aatcaaattc 721 agccgatagc ggaacgggaa ggcgactgga gtgccatgtc cggttttcaa caaaccatgc 781 aaatgctgaa tgagggcatc gttcccactg cgatgctggt tgccaacgat cagatggcgc 841 tgggcgcaat gcgcgccatt accgagtccg ggctgcgcgt tggtgcggat atctcggtag 901 tgggatacga cgataccgaa gacagctcat gttatatccc gccgttaacc accatcaaac 961 aggattttcg cctgctgggg caaaccagcg tggaccgctt gctgcaactc tctcagggcc 1021 aggcggtgaa gggcaatcag ctgttgcccg tctcactggt gaaaagaaaa accaccctgg 1081 cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac 1141 gacaggtttc ccgactggaa agcgggcagg ctgcaaacga cgaaaactac gctttagtag 1201 cttaataact ctgatagtgc tagtgtagat ctctactaga gccaggcatc aaataaaacg 1261 aaaggctcag tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct 1321 ctactagagt cacactggct caccttcggg tgggcctttc tgcgtttata tactagagaa 1381 ttgtgagcgg ataacaattg acattgtgag cggataacaa gatactgagc acatactaga 1441 gaaagaggag aaatactaga tgagcacaaa aaagaaacca ttaacacaag agcagcttga 1501 ggacgcacgt cgccttaaag caatttatga aaaaaagaaa aatgaacttg gcttatccca 1561 ggaatctgtc gcagacaaga tggggatggg gcagtcaggc gttggtgctt tatttaatgg 1621 catcaatgca ttaaatgctt ataacgccgc attgcttgca aaaattctca aagttagcgt 1681 tgaagaattt agcccttcaa tcgccagaga aatctacgag atgtatgaag cggttagtat 1741 gcagccgtca cttagaagtg agtatgagta ccctgttttt tctcatgttc aggcagggat 1801 gttctcacct gagcttagaa cctttaccaa aggtgatgcg gagagatggg taagcacaac 1861 caaaaaagcc agtgattctg cattctggct tgaggttgaa ggtaattcca tgaccgcacc 1921 aacaggctcc aagccaagct ttcctgacgg aatgttaatt ctcgttgacc ctgagcaggc 1981 tgttgagcca ggtgatttct gcatagccag acttgggggt gatgagttta ccttcaagaa 2041 actgatcagg gatagcggtc aggtgttttt acaaccacta aacccacagt acccaatgat 2101 cccatgcaat gagagttgtt ccgttgtggg gaaagttatc gctagtcagt ggcctgaaga 2161 gacgtttggc gctgcaaacg acgaaaacta cgctttagta gcttaataac gctgatagtg 2221 ctagtgtaga tcgctactag agtcacactg gctcaccttc gggtgggcct ttctgcgttt 2281 atatactaga gagagaatat aaaaagccag attattaatc cggctttttt attattttac 2341 tagagtaaca ccgtgcgtgt tgactatttt acctctggcg gtgataatgg ttgctactag 2401 agaaagagga gaaatactag atgcgtaaag gagaagaact tttcactgga gttgtcccaa 2461 ttcttgttga attagatggt gatgttaatg ggcacaaatt ttctgtcagt ggagagggtg 2521 aaggtgatgc aacatacgga aaacttaccc ttaaatttat ttgcactact ggaaaactac 2581 ctgttccatg gccaacactt gtcactactt tcggttatgg tgttcaatgc tttgcgagat 2641 acccagatca tatgaaacag catgactttt tcaagagtgc catgcccgaa ggttatgtac 2701 aggaaagaac tatatttttc aaagatgacg ggaactacaa gacacgtgct gaagtcaagt 2761 ttgaaggtga tacccttgtt aatagaatcg agttaaaagg tattgatttt aaagaagatg 2821 gaaacattct tggacacaaa ttggaataca actataactc acacaatgta tacatcatgg 2881 cagacaaaca aaagaatgga atcaaagtta acttcaaaat tagacacaac attgaagatg 2941 gaagcgttca actagcagac cattatcaac aaaatactcc aattggcgat ggccctgtcc 3001 ttttaccaga caaccattac ctgtccacac aatctgccct ttcgaaagat cccaacgaaa 3061 agagagacca catggtcctt cttgagtttg taacagctgc tgggattaca catggcatgg 3121 atgaactata caaataataa tactagagcc aggcatcaaa taaaacgaaa ggctcagtcg 3181 aaagactggg cctttcgttt tatctgttgt ttgtcggtga acgctctcta ctagagtcac 3241 actggctcac cttcgggtgg gcctttctgc gtttatatac tagaggcaac cattatcacc 3301 gccagaggta aaatagtcaa cacgcacggt gttagatatt tataaatagt ggtgatagat 3361 ttaacgttac tagagaaaga ggagaaatac tagatggctt cctccgaaga cgttatcaaa 3421 gagttcatgc gtttcaaagt tcgtatggaa ggttccgtta acggtcacga gttcgaaatc 3481 gaaggtgaag gtgaaggtcg tccgtacgaa ggtacccaga ccgctaaact gaaagttacc 3541 aaaggtggtc cgctgccgtt cgcttgggac atcctgtccc cgcagttcca gtacggttcc 3601 aaagcttacg ttaaacaccc ggctgacatc ccggactacc tgaaactgtc cttcccggaa 3661 ggtttcaaat gggaacgtgt tatgaacttc gaagacggtg gtgttgttac cgttacccag 3721 gactcctccc tgcaagacgg tgagttcatc tacaaagtta aactgcgtgg taccaacttc 3781 ccgtccgacg gtccggttat gcagaaaaaa accatgggtt gggaagcttc caccgaacgt 3841 atgtacccgg aagacggtgc tctgaaaggt gaaatcaaaa tgcgtctgaa actgaaagac 3901 ggtggtcact acgacgctga agttaaaacc acctacatgg ctaaaaaacc ggttcagctg 3961 ccgggtgctt acaaaaccga catcaaactg gacatcacct cccacaacga agactacacc 4021 atcgttgaac agtacgaacg tgctgaaggt cgtcactcca ccggtgctta ataacgctga 4081 tagtgctagt gtagatcgct actagagcca ggcatcaaat aaaacgaaag gctcagtcga 4141 aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa cgctctctac tagagtcaca 4201 ctggctcacc ttcgggtggg cctttctgcg tttata //