##gff-version 3
##sequence-region BBa_J05504 1 4236
BBa_J05504	.	terminator	1242	1321	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_J05504	.	engineered_region	1242	1321	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_J05504	.	stem_loop	1253	1296	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_J05504	.	promoter	1	54	.	+	0	ID=BBa_R0040;Name=BBa_R0040
BBa_J05504	.	engineered_region	1	54	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_J05504	.	promoter	20	25	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_J05504	.	promoter	43	48	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_J05504	.	non_covalent_binding_site	1	19	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_J05504	.	non_covalent_binding_site	26	44	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_J05504	.	terminator	4196	4236	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_J05504	.	stop_codon	4229	4229	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_J05504	.	polyA_site	4223	4236	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_J05504	.	stem_loop	4203	4222	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_J05504	.	engineered_region	4196	4236	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_J05504	.	promoter	3286	3367	.	+	0	ID=BBa_I12007;Name=BBa_I12007
BBa_J05504	.	promoter	3356	3361	.	+	0	ID=annotation937705;Name=-10;Parent=BBa_I12007
BBa_J05504	.	non_covalent_binding_site	3318	3334	.	+	0	ID=annotation937702;Name=OR2;Parent=BBa_I12007
BBa_J05504	.	non_covalent_binding_site	3294	3310	.	+	0	ID=annotation937701;Name=OR1;Parent=BBa_I12007
BBa_J05504	.	sequence_alteration	3344	3350	.	+	0	ID=annotation937704;Name=mutate to obliterate OR3;Parent=BBa_I12007
BBa_J05504	.	promoter	3333	3338	.	+	0	ID=annotation937703;Name=-35;Parent=BBa_I12007
BBa_J05504	.	CDS	81	1208	.	+	0	ID=BBa_C0012;Name=BBa_C0012
BBa_J05504	.	engineered_tag	1209	1233	.	+	0	ID=annotation2213988;Name=Help:Barcodes;Parent=BBa_C0012
BBa_J05504	.	engineered_region	81	1208	.	+	0	ID=annotation7031;Name=BBa_C0012;Parent=BBa_C0012
BBa_J05504	.	tag	1170	1208	.	+	0	ID=annotation1722;Name=LVA;Parent=BBa_C0012
BBa_J05504	.	CDS	81	1208	.	+	0	ID=annotation1723;Name=lacI-LVA;Parent=BBa_C0012
BBa_J05504	.	ribosome_entry_site	3376	3387	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_J05504	.	conserved_region	3380	3383	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_J05504	.	terminator	2292	2337	.	+	0	ID=BBa_B0011;Name=BBa_B0011
BBa_J05504	.	engineered_region	2292	2337	.	+	0	ID=annotation7019;Name=BBa_B0011;Parent=BBa_B0011
BBa_J05504	.	stem_loop	2304	2326	.	+	0	ID=annotation1683;Name=stem_loop;Parent=BBa_B0011
BBa_J05504	.	ribosome_entry_site	1442	1453	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_J05504	.	conserved_region	1446	1449	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_J05504	.	CDS	1460	2209	.	+	0	ID=BBa_C0051;Name=BBa_C0051
BBa_J05504	.	engineered_tag	2210	2234	.	+	0	ID=annotation2213991;Name=Help:Barcodes;Parent=BBa_C0051
BBa_J05504	.	CDS	1463	2170	.	+	0	ID=annotation23334;Name=cI lambda;Parent=BBa_C0051
BBa_J05504	.	tag	2171	2203	.	+	0	ID=annotation23335;Name=LVA;Parent=BBa_C0051
BBa_J05504	.	ribosome_entry_site	2403	2414	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_J05504	.	conserved_region	2407	2410	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_J05504	.	terminator	2243	2283	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_J05504	.	stop_codon	2276	2276	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_J05504	.	polyA_site	2270	2283	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_J05504	.	stem_loop	2250	2269	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_J05504	.	engineered_region	2243	2283	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_J05504	.	CDS	3394	4074	.	+	0	ID=BBa_E1010;Name=BBa_E1010
BBa_J05504	.	engineered_tag	4075	4099	.	+	0	ID=annotation2214014;Name=Help:Barcodes;Parent=BBa_E1010
BBa_J05504	.	CDS	3394	4068	.	+	0	ID=annotation1014044;Name=mrfp1;Parent=BBa_E1010
BBa_J05504	.	ribosome_entry_site	63	74	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_J05504	.	conserved_region	67	70	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_J05504	.	terminator	4108	4187	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_J05504	.	engineered_region	4108	4187	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_J05504	.	stem_loop	4119	4162	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_J05504	.	CDS	2421	3140	.	+	0	ID=BBa_E0040;Name=BBa_E0040
BBa_J05504	.	CDS	2421	3140	.	+	0	ID=annotation1934520;Name=GFP protein;Parent=BBa_E0040
BBa_J05504	.	promoter	1379	1432	.	+	0	ID=BBa_R0011;Name=BBa_R0011
BBa_J05504	.	promoter	1421	1426	.	+	0	ID=annotation2002;Name=-10;Parent=BBa_R0011
BBa_J05504	.	engineered_region	1379	1432	.	+	0	ID=annotation7064;Name=BBa_R0011;Parent=BBa_R0011
BBa_J05504	.	operator	1381	1397	.	+	0	ID=annotation1999;Name=lac O1;Parent=BBa_R0011
BBa_J05504	.	promoter	1398	1403	.	+	0	ID=annotation2000;Name=-35;Parent=BBa_R0011
BBa_J05504	.	operator	1404	1420	.	+	0	ID=annotation2001;Name=lac O1;Parent=BBa_R0011
BBa_J05504	.	terminator	3149	3228	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_J05504	.	engineered_region	3149	3228	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_J05504	.	stem_loop	3160	3203	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_J05504	.	promoter	2346	2394	.	+	0	ID=BBa_R0051;Name=BBa_R0051
BBa_J05504	.	engineered_region	2346	2394	.	+	0	ID=annotation7067;Name=BBa_R0051;Parent=BBa_R0051
BBa_J05504	.	promoter	2383	2388	.	+	0	ID=annotation2022;Name=-10;Parent=BBa_R0051
BBa_J05504	.	non_covalent_binding_site	2370	2386	.	+	0	ID=annotation2024;Name=OR1;Parent=BBa_R0051
BBa_J05504	.	promoter	2360	2365	.	+	0	ID=annotation2023;Name=-35;Parent=BBa_R0051
BBa_J05504	.	non_covalent_binding_site	2346	2362	.	+	0	ID=annotation2025;Name=OR2;Parent=BBa_R0051
BBa_J05504	.	terminator	3237	3277	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_J05504	.	stop_codon	3270	3270	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_J05504	.	polyA_site	3264	3277	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_J05504	.	stem_loop	3244	3263	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_J05504	.	engineered_region	3237	3277	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_J05504	.	terminator	1330	1370	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_J05504	.	stop_codon	1363	1363	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_J05504	.	polyA_site	1357	1370	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_J05504	.	stem_loop	1337	1356	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_J05504	.	engineered_region	1330	1370	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
>BBa_J05504
tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagaaagaggagaaatacta
gatggtgaatgtgaaaccagtaacgttatacgatgtcgcagagtatgccggtgtctcttatcagaccgtttcccgcgtg
gtgaaccaggccagccacgtttctgcgaaaacgcgggaaaaagtggaagcggcgatggcggagctgaattacattccca
accgcgtggcacaacaactggcgggcaaacagtcgttgctgattggcgttgccacctccagtctggccctgcacgcgcc
gtcgcaaattgtcgcggcgattaaatctcgcgccgatcaactgggtgccagcgtggtggtgtcgatggtagaacgaagc
ggcgtcgaagcctgtaaagcggcggtgcacaatcttctcgcgcaacgcgtcagtgggctgatcattaactatccgctgg
atgaccaggatgccattgctgtggaagctgcctgcactaatgttccggcgttatttcttgatgtctctgaccagacacc
catcaacagtattattttctcccatgaagacggtacgcgactgggcgtggagcatctggtcgcattgggtcaccagcaa
atcgcgctgttagcgggcccattaagttctgtctcggcgcgtctgcgtctggctggctggcataaatatctcactcgca
atcaaattcagccgatagcggaacgggaaggcgactggagtgccatgtccggttttcaacaaaccatgcaaatgctgaa
tgagggcatcgttcccactgcgatgctggttgccaacgatcagatggcgctgggcgcaatgcgcgccattaccgagtcc
gggctgcgcgttggtgcggatatctcggtagtgggatacgacgataccgaagacagctcatgttatatcccgccgttaa
ccaccatcaaacaggattttcgcctgctggggcaaaccagcgtggaccgcttgctgcaactctctcagggccaggcggt
gaagggcaatcagctgttgcccgtctcactggtgaaaagaaaaaccaccctggcgcccaatacgcaaaccgcctctccc
cgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcaggctgcaaacgacgaaa
actacgctttagtagcttaataactctgatagtgctagtgtagatctctactagagccaggcatcaaataaaacgaaag
gctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcac
cttcgggtgggcctttctgcgtttatatactagagaattgtgagcggataacaattgacattgtgagcggataacaaga
tactgagcacatactagagaaagaggagaaatactagatgagcacaaaaaagaaaccattaacacaagagcagcttgag
gacgcacgtcgccttaaagcaatttatgaaaaaaagaaaaatgaacttggcttatcccaggaatctgtcgcagacaaga
tggggatggggcagtcaggcgttggtgctttatttaatggcatcaatgcattaaatgcttataacgccgcattgcttgc
aaaaattctcaaagttagcgttgaagaatttagcccttcaatcgccagagaaatctacgagatgtatgaagcggttagt
atgcagccgtcacttagaagtgagtatgagtaccctgttttttctcatgttcaggcagggatgttctcacctgagctta
gaacctttaccaaaggtgatgcggagagatgggtaagcacaaccaaaaaagccagtgattctgcattctggcttgaggt
tgaaggtaattccatgaccgcaccaacaggctccaagccaagctttcctgacggaatgttaattctcgttgaccctgag
caggctgttgagccaggtgatttctgcatagccagacttgggggtgatgagtttaccttcaagaaactgatcagggata
gcggtcaggtgtttttacaaccactaaacccacagtacccaatgatcccatgcaatgagagttgttccgttgtggggaa
agttatcgctagtcagtggcctgaagagacgtttggcgctgcaaacgacgaaaactacgctttagtagcttaataacgc
tgatagtgctagtgtagatcgctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagag
agagaatataaaaagccagattattaatccggcttttttattattttactagagtaacaccgtgcgtgttgactatttt
acctctggcggtgataatggttgctactagagaaagaggagaaatactagatgcgtaaaggagaagaacttttcactgg
agttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgat
gcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcacta
ctttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcc
cgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaa
ggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattgg
aatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattag
acacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtcctt
ttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttc
ttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagagccaggcatcaaa
taaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtca
cactggctcaccttcgggtgggcctttctgcgtttatatactagaggcaaccattatcaccgccagaggtaaaatagtc
aacacgcacggtgttagatatttataaatagtggtgatagatttaacgttactagagaaagaggagaaatactagatgg
cttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcga
aatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctg
ccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccgg
actacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgt
tacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggt
ccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtg
aaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaacc
ggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaa
cagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgctactagagc
caggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
tactagagtcacactggctcaccttcgggtgggcctttctgcgtttata