LOCUS BBa_J05505 2316 bp DNA linear 29-Nov-2024 DEFINITION Lambda-lacI input system TESTER (IPTG controlled lacI+pL efficiency) ACCESSION BBa_J05505 VERSION BBa_J05505.1 FEATURES Location/Qualifiers misc_feature 1..19 /label=TetR 1 misc_feature 1..54 /label=BBa_R0040 promoter 20..25 /label=-35 misc_feature 26..44 /label=TetR 2 promoter 43..48 /label=-10 promoter 1..54 /label=BBa_R0040 misc_feature 67..70 /label=conserved RBS 63..74 /label=BBa_B0034 misc_feature 1170..1208 /label=LVA CDS 81..1208 /label=lacI-LVA misc_feature 81..1208 /label=BBa_C0012 misc_feature 1209..1233 /label=Help:Barcodes CDS 81..1208 /label=BBa_C0012 stem_loop 1253..1296 /label=stem_loop misc_feature 1242..1321 /label=BBa_B0010 terminator 1242..1321 /label=BBa_B0010 stem_loop 1337..1356 /label=T7 TE misc_feature 1363..1363 /label=stop polyA_site 1357..1370 /label=polya misc_feature 1330..1370 /label=BBa_B0012 terminator 1330..1370 /label=BBa_B0012 misc_feature 1381..1397 /label=lac O1 promoter 1398..1403 /label=-35 misc_feature 1404..1420 /label=lac O1 promoter 1421..1426 /label=-10 misc_feature 1379..1432 /label=BBa_R0011 promoter 1379..1432 /label=BBa_R0011 misc_feature 1446..1449 /label=conserved RBS 1442..1453 /label=BBa_B0034 CDS 1460..2179 /label=GFP protein CDS 1460..2179 /label=BBa_E0040 stem_loop 2199..2242 /label=stem_loop misc_feature 2188..2267 /label=BBa_B0010 terminator 2188..2267 /label=BBa_B0010 stem_loop 2283..2302 /label=T7 TE misc_feature 2309..2309 /label=stop polyA_site 2303..2316 /label=polya misc_feature 2276..2316 /label=BBa_B0012 terminator 2276..2316 /label=BBa_B0012 ORIGIN 1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag 61 agaaagagga gaaatactag atggtgaatg tgaaaccagt aacgttatac gatgtcgcag 121 agtatgccgg tgtctcttat cagaccgttt cccgcgtggt gaaccaggcc agccacgttt 181 ctgcgaaaac gcgggaaaaa gtggaagcgg cgatggcgga gctgaattac attcccaacc 241 gcgtggcaca acaactggcg ggcaaacagt cgttgctgat tggcgttgcc acctccagtc 301 tggccctgca cgcgccgtcg caaattgtcg cggcgattaa atctcgcgcc gatcaactgg 361 gtgccagcgt ggtggtgtcg atggtagaac gaagcggcgt cgaagcctgt aaagcggcgg 421 tgcacaatct tctcgcgcaa cgcgtcagtg ggctgatcat taactatccg ctggatgacc 481 aggatgccat tgctgtggaa gctgcctgca ctaatgttcc ggcgttattt cttgatgtct 541 ctgaccagac acccatcaac agtattattt tctcccatga agacggtacg cgactgggcg 601 tggagcatct ggtcgcattg ggtcaccagc aaatcgcgct gttagcgggc ccattaagtt 661 ctgtctcggc gcgtctgcgt ctggctggct ggcataaata tctcactcgc aatcaaattc 721 agccgatagc ggaacgggaa ggcgactgga gtgccatgtc cggttttcaa caaaccatgc 781 aaatgctgaa tgagggcatc gttcccactg cgatgctggt tgccaacgat cagatggcgc 841 tgggcgcaat gcgcgccatt accgagtccg ggctgcgcgt tggtgcggat atctcggtag 901 tgggatacga cgataccgaa gacagctcat gttatatccc gccgttaacc accatcaaac 961 aggattttcg cctgctgggg caaaccagcg tggaccgctt gctgcaactc tctcagggcc 1021 aggcggtgaa gggcaatcag ctgttgcccg tctcactggt gaaaagaaaa accaccctgg 1081 cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac 1141 gacaggtttc ccgactggaa agcgggcagg ctgcaaacga cgaaaactac gctttagtag 1201 cttaataact ctgatagtgc tagtgtagat ctctactaga gccaggcatc aaataaaacg 1261 aaaggctcag tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct 1321 ctactagagt cacactggct caccttcggg tgggcctttc tgcgtttata tactagagaa 1381 ttgtgagcgg ataacaattg acattgtgag cggataacaa gatactgagc acatactaga 1441 gaaagaggag aaatactaga tgcgtaaagg agaagaactt ttcactggag ttgtcccaat 1501 tcttgttgaa ttagatggtg atgttaatgg gcacaaattt tctgtcagtg gagagggtga 1561 aggtgatgca acatacggaa aacttaccct taaatttatt tgcactactg gaaaactacc 1621 tgttccatgg ccaacacttg tcactacttt cggttatggt gttcaatgct ttgcgagata 1681 cccagatcat atgaaacagc atgacttttt caagagtgcc atgcccgaag gttatgtaca 1741 ggaaagaact atatttttca aagatgacgg gaactacaag acacgtgctg aagtcaagtt 1801 tgaaggtgat acccttgtta atagaatcga gttaaaaggt attgatttta aagaagatgg 1861 aaacattctt ggacacaaat tggaatacaa ctataactca cacaatgtat acatcatggc 1921 agacaaacaa aagaatggaa tcaaagttaa cttcaaaatt agacacaaca ttgaagatgg 1981 aagcgttcaa ctagcagacc attatcaaca aaatactcca attggcgatg gccctgtcct 2041 tttaccagac aaccattacc tgtccacaca atctgccctt tcgaaagatc ccaacgaaaa 2101 gagagaccac atggtccttc ttgagtttgt aacagctgct gggattacac atggcatgga 2161 tgaactatac aaataataat actagagcca ggcatcaaat aaaacgaaag gctcagtcga 2221 aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa cgctctctac tagagtcaca 2281 ctggctcacc ttcgggtggg cctttctgcg tttata //