LOCUS       BBa_J06000              2083 bp    DNA     linear       25-Nov-2024
DEFINITION  AHL -> YFP Receiver Test Part
ACCESSION   BBa_J06000
VERSION     BBa_J06000.1  
FEATURES             Location/Qualifiers
     misc_feature    1..20
                     /label=LuxR/HSL
     promoter        115..122
                     /label=-10
     promoter        89..94
                     /label=-35
     misc_feature    128..128
                     /label=start
     misc_feature    130..149
                     /label=Putative LuxR/HSL
     misc_feature    1..151
                     /label=BBa_R0063
     promoter        1..151
                     /label=BBa_R0063
     misc_feature    164..167
                     /label=conserved
     RBS             160..171
                     /label=BBa_B0034
     misc_feature    178..179
                     /label=prefix
     misc_feature    351..351
                     /label=T
     misc_feature    669..669
                     /label=A
     CDS             178..927
                     /label=luxR
     misc_feature    178..933
                     /label=BBa_C0062
     misc_feature    934..958
                     /label=Help:Barcodes
     CDS             178..933
                     /label=BBa_C0062
     stem_loop       978..1021
                     /label=stem_loop
     misc_feature    967..1046
                     /label=BBa_B0010
     terminator      967..1046
                     /label=BBa_B0010
     stem_loop       1062..1081
                     /label=T7 TE
     misc_feature    1088..1088
                     /label=stop
     polyA_site      1082..1095
                     /label=polya
     misc_feature    1055..1095
                     /label=BBa_B0012
     terminator      1055..1095
                     /label=BBa_B0012
     misc_feature    1104..1123
                     /label=LuxR/HSL
     promoter        1123..1128
                     /label=-35
     promoter        1145..1150
                     /label=-10
     misc_feature    1156..1156
                     /label=start
     misc_feature    1104..1158
                     /label=BBa_R0062
     promoter        1104..1158
                     /label=BBa_R0062
     misc_feature    1171..1174
                     /label=conserved
     RBS             1167..1178
                     /label=BBa_B0034
     CDS             1185..1946
                     /label=YFP (LVA)
     misc_feature    1941..1946
                     /label=2
     misc_feature    1902..1940
                     /label=SsrA
     misc_feature    1426..1426
                     /label=A (Q->L)
     misc_feature    1185..1946
                     /label=BBa_E0032
     misc_feature    1185..1946
                     /label=BBa_E0032
     stem_loop       1966..2009
                     /label=stem_loop
     misc_feature    1955..2034
                     /label=BBa_B0010
     terminator      1955..2034
                     /label=BBa_B0010
     stem_loop       2050..2069
                     /label=T7 TE
     misc_feature    2076..2076
                     /label=stop
     polyA_site      2070..2083
                     /label=polya
     misc_feature    2043..2083
                     /label=BBa_B0012
     terminator      2043..2083
                     /label=BBa_B0012
ORIGIN
        1 acctgtacga tcctacaggt gcttatgtta agtaattgta ttcccagcga tacaatagtg
       61 tgacaaaaat ccaatttatt agaatcaaat gtcaatccat taccgtttta atgatatata
      121 acacgcaaaa cttgcgacaa acaataggta atactagaga aagaggagaa atactagatg
      181 aaaaacataa atgccgacga cacatacaga ataattaata aaattaaagc ttgtagaagc
      241 aataatgata ttaatcaatg cttatctgat atgactaaaa tggtacattg tgaatattat
      301 ttactcgcga tcatttatcc tcattctatg gttaaatctg atatttcaat cctagataat
      361 taccctaaaa aatggaggca atattatgat gacgctaatt taataaaata tgatcctata
      421 gtagattatt ctaactccaa tcattcacca attaattgga atatatttga aaacaatgct
      481 gtaaataaaa aatctccaaa tgtaattaaa gaagcgaaaa catcaggtct tatcactggg
      541 tttagtttcc ctattcatac ggctaacaat ggcttcggaa tgcttagttt tgcacattca
      601 gaaaaagaca actatataga tagtttattt ttacatgcgt gtatgaacat accattaatt
      661 gttccttctc tagttgataa ttatcgaaaa ataaatatag caaataataa atcaaacaac
      721 gatttaacca aaagagaaaa agaatgttta gcgtgggcat gcgaaggaaa aagctcttgg
      781 gatatttcaa aaatattagg ttgcagtgag cgtactgtca ctttccattt aaccaatgcg
      841 caaatgaaac tcaatacaac aaaccgctgc caaagtattt ctaaagcaat tttaacagga
      901 gcaattgatt gcccatactt taaaaattaa taacactgat agtgctagtg tagatcacta
      961 ctagagccag gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta
     1021 tctgttgttt gtcggtgaac gctctctact agagtcacac tggctcacct tcgggtgggc
     1081 ctttctgcgt ttatatacta gagacctgta ggatcgtaca ggtttacgca agaaaatggt
     1141 ttgttatagt cgaataaata ctagagaaag aggagaaata ctagatggtg agcaagggcg
     1201 aggagctgtt caccggggtg gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc
     1261 acaagttcag cgtgtccggc gagggcgagg gcgatgccac ctacggcaag ctgaccctga
     1321 agttcatctg caccaccggc aagctgcccg tgccctggcc caccctcgtg accaccttcg
     1381 gctacggcct gcaatgcttc gcccgctacc ccgaccacat gaagctgcac gacttcttca
     1441 agtccgccat gcccgaaggc tacgtccagg agcgcaccat cttcttcaag gacgacggca
     1501 actacaagac ccgcgccgag gtgaagttcg agggcgacac cctggtgaac cgcatcgagc
     1561 tgaagggcat cgacttcaag gaggacggca acatcctggg gcacaagctg gagtacaact
     1621 acaacagcca caacgtctat atcatggccg acaagcagaa gaacggcatc aaggtgaact
     1681 tcaagatccg ccacaacatc gaggacggca gcgtgcagct cgccgaccac taccagcaga
     1741 acacccccat cggcgacggc cccgtgctgc tgcccgacaa ccactacctg agctaccagt
     1801 ccgccctgag caaagacccc aacgagaagc gcgatcacat ggtcctgctg gagttcgtga
     1861 ccgccgccgg gatcactctc ggcatggacg agctgtacaa gaggcctgct gcaaacgacg
     1921 aaaactacgc tttagtagct taataatact agagccaggc atcaaataaa acgaaaggct
     1981 cagtcgaaag actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctctactag
     2041 agtcacactg gctcaccttc gggtgggcct ttctgcgttt ata
//