LOCUS       BBa_J06004              2099 bp    DNA     linear       27-Mar-2025
DEFINITION  AHL receiver, CI repressor hybrid
ACCESSION   BBa_J06004
VERSION     BBa_J06004.1  
FEATURES             Location/Qualifiers
     misc_feature    1..20
                     /label=LuxR/HSL
     promoter        115..122
                     /label=-10
     promoter        89..94
                     /label=-35
     misc_feature    128..128
                     /label=start
     misc_feature    130..149
                     /label=Putative LuxR/HSL
     misc_feature    1..151
                     /label=BBa_R0063
     promoter        1..151
                     /label=BBa_R0063
     misc_feature    164..167
                     /label=conserved
     RBS             160..171
                     /label=BBa_B0034
     misc_feature    178..179
                     /label=prefix
     misc_feature    351..351
                     /label=T
     misc_feature    669..669
                     /label=A
     CDS             178..927
                     /label=luxR
     misc_feature    178..933
                     /label=BBa_C0062
     misc_feature    934..958
                     /label=Help:Barcodes
     CDS             178..933
                     /label=BBa_C0062
     stem_loop       978..1021
                     /label=stem_loop
     misc_feature    967..1046
                     /label=BBa_B0010
     terminator      967..1046
                     /label=BBa_B0010
     stem_loop       1062..1081
                     /label=T7 TE
     misc_feature    1088..1088
                     /label=stop
     polyA_site      1082..1095
                     /label=polya
     misc_feature    1055..1095
                     /label=BBa_B0012
     terminator      1055..1095
                     /label=BBa_B0012
     misc_feature    1104..1123
                     /label=LuxR/HSL
     promoter        1123..1128
                     /label=-35
     promoter        1145..1150
                     /label=-10
     misc_feature    1156..1156
                     /label=start
     misc_feature    1104..1158
                     /label=BBa_R0062
     promoter        1104..1158
                     /label=BBa_R0062
     RBS             1167..1181
                     /label=RBS-1\Strong
     RBS             1174..1178
                     /label=RBS
     misc_feature    1167..1181
                     /label=BBa_B0030
     RBS             1167..1181
                     /label=BBa_B0030
     CDS             1191..1898
                     /label=cI lambda
     misc_feature    1899..1931
                     /label=LVA
     misc_feature    1938..1962
                     /label=Help:Barcodes
     CDS             1188..1937
                     /label=BBa_C0051
     stem_loop       1982..2025
                     /label=stem_loop
     misc_feature    1971..2050
                     /label=BBa_B0010
     terminator      1971..2050
                     /label=BBa_B0010
     stem_loop       2066..2085
                     /label=T7 TE
     misc_feature    2092..2092
                     /label=stop
     polyA_site      2086..2099
                     /label=polya
     misc_feature    2059..2099
                     /label=BBa_B0012
     terminator      2059..2099
                     /label=BBa_B0012
ORIGIN
        1 acctgtacga tcctacaggt gcttatgtta agtaattgta ttcccagcga tacaatagtg
       61 tgacaaaaat ccaatttatt agaatcaaat gtcaatccat taccgtttta atgatatata
      121 acacgcaaaa cttgcgacaa acaataggta atactagaga aagaggagaa atactagatg
      181 aaaaacataa atgccgacga cacatacaga ataattaata aaattaaagc ttgtagaagc
      241 aataatgata ttaatcaatg cttatctgat atgactaaaa tggtacattg tgaatattat
      301 ttactcgcga tcatttatcc tcattctatg gttaaatctg atatttcaat cctagataat
      361 taccctaaaa aatggaggca atattatgat gacgctaatt taataaaata tgatcctata
      421 gtagattatt ctaactccaa tcattcacca attaattgga atatatttga aaacaatgct
      481 gtaaataaaa aatctccaaa tgtaattaaa gaagcgaaaa catcaggtct tatcactggg
      541 tttagtttcc ctattcatac ggctaacaat ggcttcggaa tgcttagttt tgcacattca
      601 gaaaaagaca actatataga tagtttattt ttacatgcgt gtatgaacat accattaatt
      661 gttccttctc tagttgataa ttatcgaaaa ataaatatag caaataataa atcaaacaac
      721 gatttaacca aaagagaaaa agaatgttta gcgtgggcat gcgaaggaaa aagctcttgg
      781 gatatttcaa aaatattagg ttgcagtgag cgtactgtca ctttccattt aaccaatgcg
      841 caaatgaaac tcaatacaac aaaccgctgc caaagtattt ctaaagcaat tttaacagga
      901 gcaattgatt gcccatactt taaaaattaa taacactgat agtgctagtg tagatcacta
      961 ctagagccag gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta
     1021 tctgttgttt gtcggtgaac gctctctact agagtcacac tggctcacct tcgggtgggc
     1081 ctttctgcgt ttatatacta gagacctgta ggatcgtaca ggtttacgca agaaaatggt
     1141 ttgttatagt cgaataaata ctagagatta aagaggagaa atactagatg agcacaaaaa
     1201 agaaaccatt aacacaagag cagcttgagg acgcacgtcg ccttaaagca atttatgaaa
     1261 aaaagaaaaa tgaacttggc ttatcccagg aatctgtcgc agacaagatg gggatggggc
     1321 agtcaggcgt tggtgcttta tttaatggca tcaatgcatt aaatgcttat aacgccgcat
     1381 tgcttgcaaa aattctcaaa gttagcgttg aagaatttag cccttcaatc gccagagaaa
     1441 tctacgagat gtatgaagcg gttagtatgc agccgtcact tagaagtgag tatgagtacc
     1501 ctgttttttc tcatgttcag gcagggatgt tctcacctga gcttagaacc tttaccaaag
     1561 gtgatgcgga gagatgggta agcacaacca aaaaagccag tgattctgca ttctggcttg
     1621 aggttgaagg taattccatg accgcaccaa caggctccaa gccaagcttt cctgacggaa
     1681 tgttaattct cgttgaccct gagcaggctg ttgagccagg tgatttctgc atagccagac
     1741 ttgggggtga tgagtttacc ttcaagaaac tgatcaggga tagcggtcag gtgtttttac
     1801 aaccactaaa cccacagtac ccaatgatcc catgcaatga gagttgttcc gttgtgggga
     1861 aagttatcgc tagtcagtgg cctgaagaga cgtttggcgc tgcaaacgac gaaaactacg
     1921 ctttagtagc ttaataacgc tgatagtgct agtgtagatc gctactagag ccaggcatca
     1981 aataaaacga aaggctcagt cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt
     2041 gaacgctctc tactagagtc acactggctc accttcgggt gggcctttct gcgtttata
//