LOCUS BBa_J06022 803 bp DNA linear 22-Nov-2024 DEFINITION Tet repressible luxI generator ( LVA- ) ACCESSION BBa_J06022 VERSION BBa_J06022.1 FEATURES Location/Qualifiers misc_feature 1..19 /label=TetR 1 misc_feature 1..54 /label=BBa_R0040 promoter 20..25 /label=-35 misc_feature 26..44 /label=TetR 2 promoter 43..48 /label=-10 promoter 1..54 /label=BBa_R0040 RBS 63..75 /label=RBS-3\Weak RBS 69..72 /label=RBS misc_feature 63..75 /label=BBa_B0032 RBS 63..75 /label=BBa_B0032 CDS 82..666 /label=LuxI CDS 82..666 /label=BBa_C0161 stem_loop 686..729 /label=stem_loop misc_feature 675..754 /label=BBa_B0010 terminator 675..754 /label=BBa_B0010 stem_loop 770..789 /label=T7 TE misc_feature 796..796 /label=stop polyA_site 790..803 /label=polya misc_feature 763..803 /label=BBa_B0012 terminator 763..803 /label=BBa_B0012 ORIGIN 1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag 61 agtcacacag gaaagtacta gatgactata atgataaaaa aatcggattt tttggcaatt 121 ccatcggagg agtataaagg tattctaagt cttcgttatc aagtgtttaa gcaaagactt 181 gagtgggact tagttgtaga aaataacctt gaatcagatg agtatgataa ctcaaatgca 241 gaatatattt atgcttgtga tgatactgaa aatgtaagtg gatgctggcg tttattacct 301 acaacaggtg attatatgct gaaaagtgtt tttcctgaat tgcttggtca acagagtgct 361 cccaaagatc ctaatatagt cgaattaagt cgttttgctg taggtaaaaa tagctcaaag 421 ataaataact ctgctagtga aattacaatg aaactatttg aagctatata taaacacgct 481 gttagtcaag gtattacaga atatgtaaca gtaacatcaa cagcaataga gcgattttta 541 aagcgtatta aagttccttg tcatcgtatt ggagacaaag aaattcatgt attaggtgat 601 actaaatcgg ttgtattgtc tatgcctatt aatgaacagt ttaaaaaagc agtcttaaat 661 taataatact agagccaggc atcaaataaa acgaaaggct cagtcgaaag actgggcctt 721 tcgttttatc tgttgtttgt cggtgaacgc tctctactag agtcacactg gctcaccttc 781 gggtgggcct ttctgcgttt ata //