LOCUS BBa_J06024 2097 bp DNA linear 22-Dec-2024 DEFINITION AHL receiver, CI repressor hybrid ACCESSION BBa_J06024 VERSION BBa_J06024.1 FEATURES Location/Qualifiers misc_feature 1..20 /label=LuxR/HSL promoter 115..122 /label=-10 promoter 89..94 /label=-35 misc_feature 128..128 /label=start misc_feature 130..149 /label=Putative LuxR/HSL misc_feature 1..151 /label=BBa_R0063 promoter 1..151 /label=BBa_R0063 misc_feature 164..167 /label=conserved RBS 160..171 /label=BBa_B0034 misc_feature 178..179 /label=prefix misc_feature 351..351 /label=T misc_feature 669..669 /label=A CDS 178..927 /label=luxR misc_feature 178..933 /label=BBa_C0062 misc_feature 934..958 /label=Help:Barcodes CDS 178..933 /label=BBa_C0062 stem_loop 978..1021 /label=stem_loop misc_feature 967..1046 /label=BBa_B0010 terminator 967..1046 /label=BBa_B0010 stem_loop 1062..1081 /label=T7 TE misc_feature 1088..1088 /label=stop polyA_site 1082..1095 /label=polya misc_feature 1055..1095 /label=BBa_B0012 terminator 1055..1095 /label=BBa_B0012 misc_feature 1104..1123 /label=LuxR/HSL promoter 1123..1128 /label=-35 promoter 1145..1150 /label=-10 misc_feature 1156..1156 /label=start misc_feature 1104..1158 /label=BBa_R0062 promoter 1104..1158 /label=BBa_R0062 RBS 1167..1179 /label=RBS-3\Weak RBS 1173..1176 /label=RBS misc_feature 1167..1179 /label=BBa_B0032 RBS 1167..1179 /label=BBa_B0032 CDS 1189..1896 /label=cI lambda misc_feature 1897..1929 /label=LVA misc_feature 1936..1960 /label=Help:Barcodes CDS 1186..1935 /label=BBa_C0051 stem_loop 1980..2023 /label=stem_loop misc_feature 1969..2048 /label=BBa_B0010 terminator 1969..2048 /label=BBa_B0010 stem_loop 2064..2083 /label=T7 TE misc_feature 2090..2090 /label=stop polyA_site 2084..2097 /label=polya misc_feature 2057..2097 /label=BBa_B0012 terminator 2057..2097 /label=BBa_B0012 ORIGIN 1 acctgtacga tcctacaggt gcttatgtta agtaattgta ttcccagcga tacaatagtg 61 tgacaaaaat ccaatttatt agaatcaaat gtcaatccat taccgtttta atgatatata 121 acacgcaaaa cttgcgacaa acaataggta atactagaga aagaggagaa atactagatg 181 aaaaacataa atgccgacga cacatacaga ataattaata aaattaaagc ttgtagaagc 241 aataatgata ttaatcaatg cttatctgat atgactaaaa tggtacattg tgaatattat 301 ttactcgcga tcatttatcc tcattctatg gttaaatctg atatttcaat cctagataat 361 taccctaaaa aatggaggca atattatgat gacgctaatt taataaaata tgatcctata 421 gtagattatt ctaactccaa tcattcacca attaattgga atatatttga aaacaatgct 481 gtaaataaaa aatctccaaa tgtaattaaa gaagcgaaaa catcaggtct tatcactggg 541 tttagtttcc ctattcatac ggctaacaat ggcttcggaa tgcttagttt tgcacattca 601 gaaaaagaca actatataga tagtttattt ttacatgcgt gtatgaacat accattaatt 661 gttccttctc tagttgataa ttatcgaaaa ataaatatag caaataataa atcaaacaac 721 gatttaacca aaagagaaaa agaatgttta gcgtgggcat gcgaaggaaa aagctcttgg 781 gatatttcaa aaatattagg ttgcagtgag cgtactgtca ctttccattt aaccaatgcg 841 caaatgaaac tcaatacaac aaaccgctgc caaagtattt ctaaagcaat tttaacagga 901 gcaattgatt gcccatactt taaaaattaa taacactgat agtgctagtg tagatcacta 961 ctagagccag gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta 1021 tctgttgttt gtcggtgaac gctctctact agagtcacac tggctcacct tcgggtgggc 1081 ctttctgcgt ttatatacta gagacctgta ggatcgtaca ggtttacgca agaaaatggt 1141 ttgttatagt cgaataaata ctagagtcac acaggaaagt actagatgag cacaaaaaag 1201 aaaccattaa cacaagagca gcttgaggac gcacgtcgcc ttaaagcaat ttatgaaaaa 1261 aagaaaaatg aacttggctt atcccaggaa tctgtcgcag acaagatggg gatggggcag 1321 tcaggcgttg gtgctttatt taatggcatc aatgcattaa atgcttataa cgccgcattg 1381 cttgcaaaaa ttctcaaagt tagcgttgaa gaatttagcc cttcaatcgc cagagaaatc 1441 tacgagatgt atgaagcggt tagtatgcag ccgtcactta gaagtgagta tgagtaccct 1501 gttttttctc atgttcaggc agggatgttc tcacctgagc ttagaacctt taccaaaggt 1561 gatgcggaga gatgggtaag cacaaccaaa aaagccagtg attctgcatt ctggcttgag 1621 gttgaaggta attccatgac cgcaccaaca ggctccaagc caagctttcc tgacggaatg 1681 ttaattctcg ttgaccctga gcaggctgtt gagccaggtg atttctgcat agccagactt 1741 gggggtgatg agtttacctt caagaaactg atcagggata gcggtcaggt gtttttacaa 1801 ccactaaacc cacagtaccc aatgatccca tgcaatgaga gttgttccgt tgtggggaaa 1861 gttatcgcta gtcagtggcc tgaagagacg tttggcgctg caaacgacga aaactacgct 1921 ttagtagctt aataacgctg atagtgctag tgtagatcgc tactagagcc aggcatcaaa 1981 taaaacgaaa ggctcagtcg aaagactggg cctttcgttt tatctgttgt ttgtcggtga 2041 acgctctcta ctagagtcac actggctcac cttcgggtgg gcctttctgc gtttata //