LOCUS       BBa_J06032               801 bp    DNA     linear       22-Dec-2024
DEFINITION  Tet repressible luxI generator ( LVA- )
ACCESSION   BBa_J06032
VERSION     BBa_J06032.1  
FEATURES             Location/Qualifiers
     misc_feature    1..19
                     /label=TetR 1
     misc_feature    1..54
                     /label=BBa_R0040
     promoter        20..25
                     /label=-35
     misc_feature    26..44
                     /label=TetR 2
     promoter        43..48
                     /label=-10
     promoter        1..54
                     /label=BBa_R0040
     RBS             63..73
                     /label=RBS-4\Weaker
     RBS             69..72
                     /label=RBS
     misc_feature    63..73
                     /label=BBa_B0033
     RBS             63..73
                     /label=BBa_B0033
     CDS             80..664
                     /label=LuxI
     CDS             80..664
                     /label=BBa_C0161
     stem_loop       684..727
                     /label=stem_loop
     misc_feature    673..752
                     /label=BBa_B0010
     terminator      673..752
                     /label=BBa_B0010
     stem_loop       768..787
                     /label=T7 TE
     misc_feature    794..794
                     /label=stop
     polyA_site      788..801
                     /label=polya
     misc_feature    761..801
                     /label=BBa_B0012
     terminator      761..801
                     /label=BBa_B0012
ORIGIN
        1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag
       61 agtcacacag gactactaga tgactataat gataaaaaaa tcggattttt tggcaattcc
      121 atcggaggag tataaaggta ttctaagtct tcgttatcaa gtgtttaagc aaagacttga
      181 gtgggactta gttgtagaaa ataaccttga atcagatgag tatgataact caaatgcaga
      241 atatatttat gcttgtgatg atactgaaaa tgtaagtgga tgctggcgtt tattacctac
      301 aacaggtgat tatatgctga aaagtgtttt tcctgaattg cttggtcaac agagtgctcc
      361 caaagatcct aatatagtcg aattaagtcg ttttgctgta ggtaaaaata gctcaaagat
      421 aaataactct gctagtgaaa ttacaatgaa actatttgaa gctatatata aacacgctgt
      481 tagtcaaggt attacagaat atgtaacagt aacatcaaca gcaatagagc gatttttaaa
      541 gcgtattaaa gttccttgtc atcgtattgg agacaaagaa attcatgtat taggtgatac
      601 taaatcggtt gtattgtcta tgcctattaa tgaacagttt aaaaaagcag tcttaaatta
      661 ataatactag agccaggcat caaataaaac gaaaggctca gtcgaaagac tgggcctttc
      721 gttttatctg ttgtttgtcg gtgaacgctc tctactagag tcacactggc tcaccttcgg
      781 gtgggccttt ctgcgtttat a
//