LOCUS BBa_J06034 2095 bp DNA linear 25-Nov-2024 ACCESSION BBa_J06034 VERSION BBa_J06034.1 FEATURES Location/Qualifiers misc_feature 1..20 /label=LuxR/HSL promoter 115..122 /label=-10 promoter 89..94 /label=-35 misc_feature 128..128 /label=start misc_feature 130..149 /label=Putative LuxR/HSL misc_feature 1..151 /label=BBa_R0063 promoter 1..151 /label=BBa_R0063 misc_feature 164..167 /label=conserved RBS 160..171 /label=BBa_B0034 misc_feature 178..179 /label=prefix misc_feature 351..351 /label=T misc_feature 669..669 /label=A CDS 178..927 /label=luxR misc_feature 178..933 /label=BBa_C0062 misc_feature 934..958 /label=Help:Barcodes CDS 178..933 /label=BBa_C0062 stem_loop 978..1021 /label=stem_loop misc_feature 967..1046 /label=BBa_B0010 terminator 967..1046 /label=BBa_B0010 stem_loop 1062..1081 /label=T7 TE misc_feature 1088..1088 /label=stop polyA_site 1082..1095 /label=polya misc_feature 1055..1095 /label=BBa_B0012 terminator 1055..1095 /label=BBa_B0012 misc_feature 1104..1123 /label=LuxR/HSL promoter 1123..1128 /label=-35 promoter 1145..1150 /label=-10 misc_feature 1156..1156 /label=start misc_feature 1104..1158 /label=BBa_R0062 promoter 1104..1158 /label=BBa_R0062 RBS 1167..1177 /label=RBS-4\Weaker RBS 1173..1176 /label=RBS misc_feature 1167..1177 /label=BBa_B0033 RBS 1167..1177 /label=BBa_B0033 CDS 1187..1894 /label=cI lambda misc_feature 1895..1927 /label=LVA misc_feature 1934..1958 /label=Help:Barcodes CDS 1184..1933 /label=BBa_C0051 stem_loop 1978..2021 /label=stem_loop misc_feature 1967..2046 /label=BBa_B0010 terminator 1967..2046 /label=BBa_B0010 stem_loop 2062..2081 /label=T7 TE misc_feature 2088..2088 /label=stop polyA_site 2082..2095 /label=polya misc_feature 2055..2095 /label=BBa_B0012 terminator 2055..2095 /label=BBa_B0012 ORIGIN 1 acctgtacga tcctacaggt gcttatgtta agtaattgta ttcccagcga tacaatagtg 61 tgacaaaaat ccaatttatt agaatcaaat gtcaatccat taccgtttta atgatatata 121 acacgcaaaa cttgcgacaa acaataggta atactagaga aagaggagaa atactagatg 181 aaaaacataa atgccgacga cacatacaga ataattaata aaattaaagc ttgtagaagc 241 aataatgata ttaatcaatg cttatctgat atgactaaaa tggtacattg tgaatattat 301 ttactcgcga tcatttatcc tcattctatg gttaaatctg atatttcaat cctagataat 361 taccctaaaa aatggaggca atattatgat gacgctaatt taataaaata tgatcctata 421 gtagattatt ctaactccaa tcattcacca attaattgga atatatttga aaacaatgct 481 gtaaataaaa aatctccaaa tgtaattaaa gaagcgaaaa catcaggtct tatcactggg 541 tttagtttcc ctattcatac ggctaacaat ggcttcggaa tgcttagttt tgcacattca 601 gaaaaagaca actatataga tagtttattt ttacatgcgt gtatgaacat accattaatt 661 gttccttctc tagttgataa ttatcgaaaa ataaatatag caaataataa atcaaacaac 721 gatttaacca aaagagaaaa agaatgttta gcgtgggcat gcgaaggaaa aagctcttgg 781 gatatttcaa aaatattagg ttgcagtgag cgtactgtca ctttccattt aaccaatgcg 841 caaatgaaac tcaatacaac aaaccgctgc caaagtattt ctaaagcaat tttaacagga 901 gcaattgatt gcccatactt taaaaattaa taacactgat agtgctagtg tagatcacta 961 ctagagccag gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta 1021 tctgttgttt gtcggtgaac gctctctact agagtcacac tggctcacct tcgggtgggc 1081 ctttctgcgt ttatatacta gagacctgta ggatcgtaca ggtttacgca agaaaatggt 1141 ttgttatagt cgaataaata ctagagtcac acaggactac tagatgagca caaaaaagaa 1201 accattaaca caagagcagc ttgaggacgc acgtcgcctt aaagcaattt atgaaaaaaa 1261 gaaaaatgaa cttggcttat cccaggaatc tgtcgcagac aagatgggga tggggcagtc 1321 aggcgttggt gctttattta atggcatcaa tgcattaaat gcttataacg ccgcattgct 1381 tgcaaaaatt ctcaaagtta gcgttgaaga atttagccct tcaatcgcca gagaaatcta 1441 cgagatgtat gaagcggtta gtatgcagcc gtcacttaga agtgagtatg agtaccctgt 1501 tttttctcat gttcaggcag ggatgttctc acctgagctt agaaccttta ccaaaggtga 1561 tgcggagaga tgggtaagca caaccaaaaa agccagtgat tctgcattct ggcttgaggt 1621 tgaaggtaat tccatgaccg caccaacagg ctccaagcca agctttcctg acggaatgtt 1681 aattctcgtt gaccctgagc aggctgttga gccaggtgat ttctgcatag ccagacttgg 1741 gggtgatgag tttaccttca agaaactgat cagggatagc ggtcaggtgt ttttacaacc 1801 actaaaccca cagtacccaa tgatcccatg caatgagagt tgttccgttg tggggaaagt 1861 tatcgctagt cagtggcctg aagagacgtt tggcgctgca aacgacgaaa actacgcttt 1921 agtagcttaa taacgctgat agtgctagtg tagatcgcta ctagagccag gcatcaaata 1981 aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta tctgttgttt gtcggtgaac 2041 gctctctact agagtcacac tggctcacct tcgggtgggc ctttctgcgt ttata //