##gff-version 3
##sequence-region BBa_J06035 1 1021
BBa_J06035	.	promoter	1	97	.	+	0	ID=BBa_R0065;Name=BBa_R0065
BBa_J06035	.	promoter	94	97	.	+	0	ID=annotation1986781;Name=-10;Parent=BBa_R0065
BBa_J06035	.	non_covalent_binding_site	81	97	.	+	0	ID=annotation1986780;Name=OR1 cI;Parent=BBa_R0065
BBa_J06035	.	promoter	47	52	.	+	0	ID=annotation1986776;Name=-10;Parent=BBa_R0065
BBa_J06035	.	non_covalent_binding_site	6	25	.	+	0	ID=annotation1986775;Name=Lux Box;Parent=BBa_R0065
BBa_J06035	.	promoter	71	76	.	+	0	ID=annotation1986779;Name=-35;Parent=BBa_R0065
BBa_J06035	.	promoter	58	58	.	+	0	ID=annotation1986778;Name=lux p(R) start;Parent=BBa_R0065
BBa_J06035	.	non_covalent_binding_site	57	73	.	+	0	ID=annotation1986777;Name=OR2 cI;Parent=BBa_R0065
BBa_J06035	.	engineered_region	1	97	.	+	0	ID=annotation1986774;Name=BBa_R0065;Parent=BBa_R0065
BBa_J06035	.	ribosome_entry_site	106	116	.	+	0	ID=BBa_B0033;Name=BBa_B0033
BBa_J06035	.	ribosome_entry_site	112	115	.	+	0	ID=annotation1714;Name=RBS;Parent=BBa_B0033
BBa_J06035	.	ribosome_entry_site	106	116	.	+	0	ID=annotation1713;Name=RBS-4\Weaker;Parent=BBa_B0033
BBa_J06035	.	engineered_region	106	116	.	+	0	ID=annotation7028;Name=BBa_B0033;Parent=BBa_B0033
BBa_J06035	.	engineered_region	123	884	.	+	0	ID=BBa_E0032;Name=BBa_E0032
BBa_J06035	.	CDS	123	884	.	+	0	ID=annotation2156;Name=YFP (LVA);Parent=BBa_E0032
BBa_J06035	.	tag	840	878	.	+	0	ID=annotation2160;Name=SsrA;Parent=BBa_E0032
BBa_J06035	.	engineered_region	123	884	.	+	0	ID=annotation7041;Name=BBa_E0032;Parent=BBa_E0032
BBa_J06035	.	stop_codon	879	884	.	+	0	ID=annotation2159;Name=2;Parent=BBa_E0032
BBa_J06035	.	sequence_alteration	364	364	.	+	0	ID=annotation2161;Name=A (Q->L);Parent=BBa_E0032
BBa_J06035	.	terminator	893	972	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_J06035	.	stem_loop	904	947	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_J06035	.	engineered_region	893	972	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_J06035	.	terminator	981	1021	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_J06035	.	engineered_region	981	1021	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_J06035	.	stop_codon	1014	1014	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_J06035	.	stem_loop	988	1007	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_J06035	.	polyA_site	1008	1021	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
>BBa_J06035
taagcacctgtaggatcgtacaggtttacgcaagaaaatggtttgttatagtcgaataacaccgtgcgtgttgactatt
ttacctctggcggtgatatactagagtcacacaggactactagatggtgagcaagggcgaggagctgttcaccggggtg
gtgcccatcctggtcgagctggacggcgacgtaaacggccacaagttcagcgtgtccggcgagggcgagggcgatgcca
cctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccacctt
cggctacggcctgcaatgcttcgcccgctaccccgaccacatgaagctgcacgacttcttcaagtccgccatgcccgaa
ggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcg
acaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctggagta
caactacaacagccacaacgtctatatcatggccgacaagcagaagaacggcatcaaggtgaacttcaagatccgccac
aacatcgaggacggcagcgtgcagctcgccgaccactaccagcagaacacccccatcggcgacggccccgtgctgctgc
ccgacaaccactacctgagctaccagtccgccctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctgga
gttcgtgaccgccgccgggatcactctcggcatggacgagctgtacaagaggcctgctgcaaacgacgaaaactacgct
ttagtagcttaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatc
tgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata