LOCUS BBa_J06042 805 bp DNA linear 22-Nov-2024 DEFINITION Tet repressible luxI generator ( LVA- ) ACCESSION BBa_J06042 VERSION BBa_J06042.1 FEATURES Location/Qualifiers misc_feature 1..19 /label=TetR 1 misc_feature 1..54 /label=BBa_R0040 promoter 20..25 /label=-35 misc_feature 26..44 /label=TetR 2 promoter 43..48 /label=-10 promoter 1..54 /label=BBa_R0040 RBS 63..77 /label=RBS-1\Strong RBS 70..74 /label=RBS misc_feature 63..77 /label=BBa_B0030 RBS 63..77 /label=BBa_B0030 CDS 84..668 /label=LuxI CDS 84..668 /label=BBa_C0161 stem_loop 688..731 /label=stem_loop misc_feature 677..756 /label=BBa_B0010 terminator 677..756 /label=BBa_B0010 stem_loop 772..791 /label=T7 TE misc_feature 798..798 /label=stop polyA_site 792..805 /label=polya misc_feature 765..805 /label=BBa_B0012 terminator 765..805 /label=BBa_B0012 ORIGIN 1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag 61 agattaaaga ggagaaatac tagatgacta taatgataaa aaaatcggat tttttggcaa 121 ttccatcgga ggagtataaa ggtattctaa gtcttcgtta tcaagtgttt aagcaaagac 181 ttgagtggga cttagttgta gaaaataacc ttgaatcaga tgagtatgat aactcaaatg 241 cagaatatat ttatgcttgt gatgatactg aaaatgtaag tggatgctgg cgtttattac 301 ctacaacagg tgattatatg ctgaaaagtg tttttcctga attgcttggt caacagagtg 361 ctcccaaaga tcctaatata gtcgaattaa gtcgttttgc tgtaggtaaa aatagctcaa 421 agataaataa ctctgctagt gaaattacaa tgaaactatt tgaagctata tataaacacg 481 ctgttagtca aggtattaca gaatatgtaa cagtaacatc aacagcaata gagcgatttt 541 taaagcgtat taaagttcct tgtcatcgta ttggagacaa agaaattcat gtattaggtg 601 atactaaatc ggttgtattg tctatgccta ttaatgaaca gtttaaaaaa gcagtcttaa 661 attaataata ctagagccag gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc 721 tttcgtttta tctgttgttt gtcggtgaac gctctctact agagtcacac tggctcacct 781 tcgggtgggc ctttctgcgt ttata //