##gff-version 3
##sequence-region BBa_J06100 1 2072
BBa_J06100	.	terminator	1944	2023	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_J06100	.	stem_loop	1955	1998	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_J06100	.	engineered_region	1944	2023	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_J06100	.	promoter	991	1147	.	+	0	ID=BBa_R0079;Name=BBa_R0079
BBa_J06100	.	non_covalent_binding_site	1036	1053	.	+	0	ID=annotation300985;Name=OP2;Parent=BBa_R0079
BBa_J06100	.	promoter	1107	1112	.	+	0	ID=annotation318495;Name=-35;Parent=BBa_R0079
BBa_J06100	.	promoter	1130	1135	.	+	0	ID=annotation318496;Name=-10;Parent=BBa_R0079
BBa_J06100	.	non_covalent_binding_site	1096	1115	.	+	0	ID=annotation300992;Name=OP1;Parent=BBa_R0079
BBa_J06100	.	terminator	854	933	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_J06100	.	stem_loop	865	908	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_J06100	.	engineered_region	854	933	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_J06100	.	CDS	65	820	.	+	0	ID=BBa_C0079;Name=BBa_C0079
BBa_J06100	.	engineered_tag	821	845	.	+	0	ID=annotation2213999;Name=Help:Barcodes;Parent=BBa_C0079
BBa_J06100	.	tag	782	814	.	+	0	ID=annotation307948;Name=LVA;Parent=BBa_C0079
BBa_J06100	.	CDS	65	781	.	+	0	ID=annotation307935;Name=lasR;Parent=BBa_C0079
BBa_J06100	.	sequence_alteration	544	544	.	+	0	ID=annotation308006;Name=G;Parent=BBa_C0079
BBa_J06100	.	ribosome_entry_site	47	58	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_J06100	.	conserved_region	51	54	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_J06100	.	terminator	942	982	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_J06100	.	polyA_site	969	982	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_J06100	.	engineered_region	942	982	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_J06100	.	stem_loop	949	968	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_J06100	.	stop_codon	975	975	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_J06100	.	promoter	1	38	.	+	0	ID=BBa_I14033;Name=BBa_I14033
BBa_J06100	.	promoter	4	9	.	+	0	ID=annotation1026172;Name=-35;Parent=BBa_I14033
BBa_J06100	.	operator	1	38	.	+	0	ID=annotation1026174;Name=P(Cat);Parent=BBa_I14033
BBa_J06100	.	promoter	27	32	.	+	0	ID=annotation1026173;Name=-10;Parent=BBa_I14033
BBa_J06100	.	engineered_region	1174	1935	.	+	0	ID=BBa_E0032;Name=BBa_E0032
BBa_J06100	.	stop_codon	1930	1935	.	+	0	ID=annotation2159;Name=2;Parent=BBa_E0032
BBa_J06100	.	CDS	1174	1935	.	+	0	ID=annotation2156;Name=YFP (LVA);Parent=BBa_E0032
BBa_J06100	.	sequence_alteration	1415	1415	.	+	0	ID=annotation2161;Name=A (Q->L);Parent=BBa_E0032
BBa_J06100	.	engineered_region	1174	1935	.	+	0	ID=annotation7041;Name=BBa_E0032;Parent=BBa_E0032
BBa_J06100	.	tag	1891	1929	.	+	0	ID=annotation2160;Name=SsrA;Parent=BBa_E0032
BBa_J06100	.	terminator	2032	2072	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_J06100	.	polyA_site	2059	2072	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_J06100	.	engineered_region	2032	2072	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_J06100	.	stem_loop	2039	2058	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_J06100	.	stop_codon	2065	2065	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_J06100	.	ribosome_entry_site	1156	1167	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_J06100	.	conserved_region	1160	1163	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
>BBa_J06100
ggcacgtaagaggttccaactttcaccataatgaaacatactagagaaagaggagaaatactagatggccttggttgac
ggttttcttgagctggaacgctcaagtggaaaattggagtggagcgccatcctccagaagatggcgagcgaccttggat
tctcgaagatcctgttcggcctgttgcctaaggacagccaggactacgagaacgccttcatcgtcggcaactacccggc
cgcctggcgcgagcattacgaccgggctggctacgcgcgggtcgacccgacggtcagtcactgtacccagagcgtactg
ccgattttctgggaaccgtccatctaccagacgcgaaagcagcacgagttcttcgaggaagcctcggccgccggcctgg
tgtatgggctgaccatgccgctgcatggtgctcgcggcgaactcggcgcgctgagcctcagcgtggaagcggaaaaccg
ggccgaggccaaccgtttcatagagtcggtcctgccgaccctgtggatgctcaaggactacgcactgcaaagcggtgcc
ggactggccttcgaacatccggtcagcaaaccggtggttctgaccagccgggagaaggaagtgttgcagtggtgcgcca
tcggcaagaccagttgggagatatcggttatctgcaactgctcggaagccaatgtgaacttccatatgggaaatattcg
gcggaagttcggtgtgacctcccgccgcgtagcggccattatggccgttaatttgggtcttattactctcgctgcaaac
gacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcactactagagccaggcatcaaataaa
acgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacact
ggctcaccttcgggtgggcctttctgcgtttatatactagaggcccctcgctgagcgcgtcccggagctgggggcaacc
tagctgccacctgcttttctgctagctattccagcgaaaacatacagatttccggcgaaatcaaggctacctgccagtt
ctggcaggtttggccgcgggttctttttggtacacgaaagctactagagaaagaggagaaatactagatggtgagcaag
ggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtaaacggccacaagttcagcgtgt
ccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgcc
ctggcccaccctcgtgaccaccttcggctacggcctgcaatgcttcgcccgctaccccgaccacatgaagctgcacgac
ttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagaccc
gcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaa
catcctggggcacaagctggagtacaactacaacagccacaacgtctatatcatggccgacaagcagaagaacggcatc
aaggtgaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagcagaacaccccca
tcggcgacggccccgtgctgctgcccgacaaccactacctgagctaccagtccgccctgagcaaagaccccaacgagaa
gcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgtacaagaggcct
gctgcaaacgacgaaaactacgctttagtagcttaataatactagagccaggcatcaaataaaacgaaaggctcagtcg
aaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtg
ggcctttctgcgtttata